SARS-CoV-2 E Gene Tagged ORF (codon optimized)

CAT#: VC102565

Myc-DDK-tagged ORF clone for SARS-CoV-2 Envelop Protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724392

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: E
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102565 represents NCBI reference of YP_009724392 with codon optimized for human cell expression
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTATTCCTTCGTGTCTGAAGAAACTGGTACCCTCATTGTCAATTCTGTCTTGCTGTTCCTCGCCTTC
  GTCGTCTTCCTCCTTGTCACCCTTGCCATTCTTACTGCTCTCCGCCTCTGTGCCTATTGTTGTAATATC
  GTTAATGTCTCCCTGGTTAAACCTTCTTTCTATGTCTACTCCCGCGTAAAAAACCTCAACTCCTCACGG
  GTTCCCGATCTCCTTGTG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >VC102565 representing YP_009724392
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCNIVNVSLVKPSFYVYSRVKNLNSSR
  VPDLLV
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_045512
• ORF Size: 225 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NC_045512.2, YP_009724392
• RefSeq ORF: 225
• MW: 8.4 kDa