SARS-CoV-2 M Gene Tagged ORF (codon optimized)

CAT#: VC102559

Myc-DDK-tagged ORF clone for SARS-CoV-2 Membrane Glycoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724393

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: M Protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102559 represents NCBI reference of YP_009724393 with codon optimized for human cell expression
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCGACTCAAATGGGACAATCACCGTCGAAGAACTGAAGAAACTTCTGGAACAGTGGAACCTCGTT
  ATCGGCTTTCTCTTTCTCACCTGGATCTGCTTGCTCCAGTTTGCATACGCCAACCGAAATCGATTTCTT
  TATATCATCAAACTCATCTTCCTTTGGTTGCTCTGGCCCGTCACCCTCGCATGTTTCGTTTTGGCTGCA
  GTGTATAGAATAAATTGGATTACCGGCGGAATTGCTATTGCTATGGCTTGTCTTGTGGGACTTATGTGG
  CTCAGTTACTTTATTGCTTCCTTTAGATTGTTTGCCCGGACTCGCTCAATGTGGTCTTTTAACCCTGAA
  ACTAACATCCTCCTTAACGTCCCCCTTCATGGCACAATCCTGACGCGACCCCTCCTCGAAAGTGAGCTG
  GTGATCGGCGCTGTAATCCTGCGAGGCCATCTCAGAATCGCCGGGCACCATCTCGGCCGCTGCGATATA
  AAAGACCTGCCCAAAGAAATTACAGTTGCAACGTCTCGCACCCTTTCCTATTATAAGTTGGGAGCTAGT
  CAGAGAGTCGCAGGAGACTCTGGTTTTGCCGCATACTCTCGATACCGCATTGGAAACTACAAGCTTAAC
  ACCGACCACTCATCATCTTCCGACAACATCGCCCTGCTCGTTCAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  
  Protein Sequence

  >VC102559 representing YP_009724393
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MADSNGTITVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNRFLYIIKLIFLWLLWPVTLACFVLAA
  VYRINWITGGIAIAMACLVGLMWLSYFIASFRLFARTRSMWSFNPETNILLNVPLHGTILTRPLLESEL
  VIGAVILRGHLRIAGHHLGRCDIKDLPKEITVATSRTLSYYKLGASQRVAGDSGFAAYSRYRIGNYKLN
  TDHSSSSDNIALLVQ
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_045512
• ORF Size: 666 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_045512.2, YP_009724393
• RefSeq ORF: 666 bp
• MW: 25.1 kDa