SARS-CoV-2 S Gene Tagged ORF (codon optimized)

CAT#: VC102557

Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724390

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: S Protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102557 represents NCBI reference of YP_009724390 with codon optimized for human cell expression
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTTGTGTTTCTCGTCCTGCTGCCTCTGGTCTCCTCACAGTGCGTGAATCTGACAACGAGAACTCAG
  CTCCCACCCGCATATACCAATTCTTTCACCCGGGGTGTGTACTACCCTGACAAAGTGTTCAGAAGCTCA
  GTACTTCATTCCACTCAGGACCTGTTTCTTCCATTTTTCAGCAATGTTACCTGGTTTCACGCCATCCAC
  GTATCCGGGACCAACGGCACGAAACGCTTTGATAACCCGGTCCTGCCATTCAACGACGGGGTCTATTTT
  GCTTCAACTGAAAAAAGCAACATTATCAGAGGCTGGATCTTCGGCACAACTCTGGACTCAAAGACTCAG
  TCATTGCTCATTGTCAATAACGCAACCAACGTTGTGATCAAGGTCTGTGAATTCCAGTTCTGCAACGAT
  CCGTTTCTGGGCGTATACTACCACAAGAACAACAAATCTTGGATGGAGAGTGAATTTCGAGTTTATTCC
  AGCGCGAACAACTGCACCTTCGAATACGTTAGTCAGCCCTTCCTCATGGACCTTGAGGGGAAACAAGGC
  AATTTTAAAAATCTGCGCGAATTCGTATTCAAAAACATCGATGGCTACTTTAAAATTTACTCCAAACAC
  ACTCCGATCAACCTGGTTAGGGACCTGCCCCAGGGCTTCTCCGCTCTGGAGCCTCTTGTGGACCTGCCC
  ATTGGAATTAATATCACCCGCTTTCAGACGCTGCTCGCCCTGCATCGGTCTTATCTGACTCCCGGCGAT
  TCCTCATCAGGTTGGACCGCTGGTGCTGCGGCCTATTACGTCGGATATTTGCAGCCTCGGACATTCCTT
  CTGAAATACAATGAGAATGGAACAATTACAGATGCTGTGGACTGTGCACTCGACCCGTTGTCCGAAACC
  AAATGCACCTTGAAAAGCTTTACTGTGGAAAAGGGCATCTATCAGACATCTAACTTCCGGGTTCAACCT
  ACCGAATCTATTGTCCGCTTCCCCAATATTACCAACCTTTGTCCCTTCGGTGAGGTATTCAACGCAACT
  CGGTTTGCCTCAGTCTACGCATGGAACCGCAAGAGGATTTCCAATTGCGTTGCCGATTATTCAGTCCTT
  TACAACTCCGCAAGCTTTTCCACGTTTAAATGCTACGGAGTCTCCCCAACAAAACTCAACGACCTGTGC
  TTTACCAATGTCTACGCAGACTCCTTTGTTATCCGCGGCGACGAGGTTAGGCAAATTGCCCCAGGGCAG
  ACTGGCAAGATCGCCGATTATAACTATAAGCTCCCCGATGACTTTACAGGCTGCGTGATCGCATGGAAC
  TCTAACAACCTCGATAGTAAGGTGGGGGGCAATTACAATTATCTTTACCGGCTGTTTCGAAAGTCAAAC
  CTTAAACCCTTCGAGCGCGATATTTCAACCGAAATTTATCAGGCCGGGAGCACACCATGCAACGGAGTG
  GAGGGTTTCAACTGCTACTTCCCACTGCAGTCTTACGGCTTCCAGCCCACCAATGGTGTGGGTTATCAG
  CCTTACAGAGTTGTTGTGCTCAGTTTCGAGCTCCTCCACGCCCCAGCTACCGTTTGCGGACCTAAGAAA
  AGCACAAACCTGGTCAAAAATAAATGCGTAAACTTCAACTTTAACGGTCTGACTGGTACCGGCGTTCTC
  ACCGAGTCCAATAAGAAATTCTTGCCATTTCAACAGTTTGGTAGAGACATCGCTGACACCACCGATGCT
  GTCCGGGATCCCCAGACCCTTGAGATTCTCGACATAACGCCATGCTCATTCGGGGGTGTCAGCGTTATC
  ACTCCGGGCACAAACACTAGCAATCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACTGAAGTACCC
  GTGGCCATTCACGCCGATCAGCTGACGCCAACCTGGAGAGTCTACTCAACAGGCTCTAACGTTTTTCAG
  ACAAGAGCCGGTTGTTTGATCGGAGCCGAACACGTAAATAATTCATATGAATGTGACATCCCAATCGGG
  GCCGGGATCTGCGCTAGTTACCAGACCCAGACTAACTCACCACGGAGAGCCAGAAGTGTAGCCTCCCAG
  TCAATAATCGCCTATACAATGTCTTTGGGCGCGGAAAATAGCGTGGCCTACTCCAATAATTCCATAGCC
  ATTCCCACTAACTTCACAATTTCCGTCACGACCGAGATCCTGCCGGTGTCAATGACAAAAACCTCTGTG
  GACTGCACAATGTATATATGTGGCGACAGTACCGAGTGTTCTAATCTCCTTTTGCAGTATGGGTCCTTC
  TGCACACAGCTGAATCGCGCTCTTACTGGTATCGCTGTGGAGCAAGATAAAAACACGCAGGAAGTGTTC
  GCTCAAGTGAAGCAGATTTATAAAACCCCTCCAATCAAAGATTTCGGCGGATTTAACTTCTCACAGATT
  CTCCCTGATCCAAGTAAACCCTCCAAAAGAAGTTTCATAGAGGACCTCCTGTTCAACAAGGTTACGCTG
  GCTGATGCTGGTTTCATCAAGCAGTATGGGGACTGCCTCGGCGACATAGCTGCTCGAGATTTGATCTGC
  GCCCAGAAATTCAATGGGCTGACAGTGCTGCCACCTCTCTTGACAGACGAAATGATTGCACAATATACT
  AGTGCCCTTTTGGCTGGCACCATCACATCCGGTTGGACTTTTGGTGCGGGAGCCGCACTCCAAATACCC
  TTCGCAATGCAGATGGCATACAGATTCAATGGCATTGGCGTCACCCAGAACGTTCTCTACGAAAACCAG
  AAACTGATAGCAAATCAGTTTAACAGCGCTATCGGCAAGATCCAGGACTCCCTCTCATCAACTGCCTCA
  GCCCTGGGCAAGCTCCAGGATGTGGTAAACCAAAACGCCCAGGCCCTCAACACTCTGGTCAAGCAGCTC
  AGTTCTAACTTCGGAGCCATCTCTAGTGTTCTCAATGACATCCTCTCTCGGCTGGACAAGGTGGAAGCA
  GAGGTGCAGATCGACCGCCTGATTACTGGACGCCTGCAGAGCCTCCAGACCTACGTAACCCAGCAACTC
  ATCCGGGCTGCCGAGATTAGGGCTTCCGCGAACCTCGCCGCCACAAAGATGAGCGAGTGCGTACTTGGA
  CAGAGCAAGAGAGTGGATTTTTGTGGCAAGGGGTATCATCTCATGTCCTTCCCTCAGAGCGCTCCTCAC
  GGTGTCGTATTTCTCCATGTGACTTATGTCCCCGCCCAAGAGAAAAACTTCACAACCGCTCCTGCTATC
  TGCCACGACGGCAAAGCCCATTTTCCTCGGGAGGGAGTGTTCGTCTCTAATGGGACTCACTGGTTTGTT
  ACGCAGAGGAACTTCTACGAGCCTCAGATCATCACAACAGATAACACCTTTGTGTCTGGTAACTGTGAC
  GTGGTCATCGGCATAGTAAATAACACCGTCTACGACCCACTGCAGCCTGAACTGGATAGCTTTAAGGAA
  GAGCTCGATAAGTACTTCAAAAACCACACAAGCCCGGATGTAGATCTTGGAGACATTAGTGGGATTAAC
  GCCAGCGTCGTTAACATACAAAAAGAGATTGACCGATTGAACGAAGTTGCCAAAAACCTGAATGAATCC
  CTGATCGATCTGCAGGAGCTCGGCAAGTACGAGCAGTATATTAAATGGCCCTGGTACATTTGGCTGGGC
  TTCATCGCGGGGCTCATCGCGATTGTGATGGTGACCATTATGCTGTGTTGCATGACCAGTTGCTGTAGC
  TGTCTCAAAGGATGCTGCTCATGTGGCAGCTGTTGCAAGTTCGACGAGGATGATTCCGAACCCGTACTG
  AAAGGCGTGAAACTCCATTATACT
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >VC102557 representing YP_009724390
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIH
  VSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCND
  PFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKH
  TPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFL
  LKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNAT
  RFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQ
  TGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGV
  EGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVL
  TESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVP
  VAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQ
  SIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSF
  CTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTL
  ADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIP
  FAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQL
  SSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLG
  QSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFV
  TQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGIN
  ASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCS
  CLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_045512
• ORF Size: 3819 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_045512.2, YP_009724390
• RefSeq ORF: 3819 bp
• Locus ID: 43740568
• MW: 141.2 kDa