SARS-CoV-2 S Gene Tagged ORF (codon optimized)

CAT#: VC102557

  • TrueORF®

Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724390


  View other clones from "SARS-CoV-2" (40)

Reconstitution Protocol

USD 1,860.00

3 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Tag Myc-DDK
Symbol S Protein
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102557 represents NCBI reference of YP_009724390 with codon optimized for human cell expression
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTTTGTGTTTCTCGTCCTGCTGCCTCTGGTCTCCTCACAGTGCGTGAATCTGACAACGAGAACTCAG
CTCCCACCCGCATATACCAATTCTTTCACCCGGGGTGTGTACTACCCTGACAAAGTGTTCAGAAGCTCA
GTACTTCATTCCACTCAGGACCTGTTTCTTCCATTTTTCAGCAATGTTACCTGGTTTCACGCCATCCAC
GTATCCGGGACCAACGGCACGAAACGCTTTGATAACCCGGTCCTGCCATTCAACGACGGGGTCTATTTT
GCTTCAACTGAAAAAAGCAACATTATCAGAGGCTGGATCTTCGGCACAACTCTGGACTCAAAGACTCAG
TCATTGCTCATTGTCAATAACGCAACCAACGTTGTGATCAAGGTCTGTGAATTCCAGTTCTGCAACGAT
CCGTTTCTGGGCGTATACTACCACAAGAACAACAAATCTTGGATGGAGAGTGAATTTCGAGTTTATTCC
AGCGCGAACAACTGCACCTTCGAATACGTTAGTCAGCCCTTCCTCATGGACCTTGAGGGGAAACAAGGC
AATTTTAAAAATCTGCGCGAATTCGTATTCAAAAACATCGATGGCTACTTTAAAATTTACTCCAAACAC
ACTCCGATCAACCTGGTTAGGGACCTGCCCCAGGGCTTCTCCGCTCTGGAGCCTCTTGTGGACCTGCCC
ATTGGAATTAATATCACCCGCTTTCAGACGCTGCTCGCCCTGCATCGGTCTTATCTGACTCCCGGCGAT
TCCTCATCAGGTTGGACCGCTGGTGCTGCGGCCTATTACGTCGGATATTTGCAGCCTCGGACATTCCTT
CTGAAATACAATGAGAATGGAACAATTACAGATGCTGTGGACTGTGCACTCGACCCGTTGTCCGAAACC
AAATGCACCTTGAAAAGCTTTACTGTGGAAAAGGGCATCTATCAGACATCTAACTTCCGGGTTCAACCT
ACCGAATCTATTGTCCGCTTCCCCAATATTACCAACCTTTGTCCCTTCGGTGAGGTATTCAACGCAACT
CGGTTTGCCTCAGTCTACGCATGGAACCGCAAGAGGATTTCCAATTGCGTTGCCGATTATTCAGTCCTT
TACAACTCCGCAAGCTTTTCCACGTTTAAATGCTACGGAGTCTCCCCAACAAAACTCAACGACCTGTGC
TTTACCAATGTCTACGCAGACTCCTTTGTTATCCGCGGCGACGAGGTTAGGCAAATTGCCCCAGGGCAG
ACTGGCAAGATCGCCGATTATAACTATAAGCTCCCCGATGACTTTACAGGCTGCGTGATCGCATGGAAC
TCTAACAACCTCGATAGTAAGGTGGGGGGCAATTACAATTATCTTTACCGGCTGTTTCGAAAGTCAAAC
CTTAAACCCTTCGAGCGCGATATTTCAACCGAAATTTATCAGGCCGGGAGCACACCATGCAACGGAGTG
GAGGGTTTCAACTGCTACTTCCCACTGCAGTCTTACGGCTTCCAGCCCACCAATGGTGTGGGTTATCAG
CCTTACAGAGTTGTTGTGCTCAGTTTCGAGCTCCTCCACGCCCCAGCTACCGTTTGCGGACCTAAGAAA
AGCACAAACCTGGTCAAAAATAAATGCGTAAACTTCAACTTTAACGGTCTGACTGGTACCGGCGTTCTC
ACCGAGTCCAATAAGAAATTCTTGCCATTTCAACAGTTTGGTAGAGACATCGCTGACACCACCGATGCT
GTCCGGGATCCCCAGACCCTTGAGATTCTCGACATAACGCCATGCTCATTCGGGGGTGTCAGCGTTATC
ACTCCGGGCACAAACACTAGCAATCAGGTGGCCGTGCTGTACCAGGACGTGAACTGCACTGAAGTACCC
GTGGCCATTCACGCCGATCAGCTGACGCCAACCTGGAGAGTCTACTCAACAGGCTCTAACGTTTTTCAG
ACAAGAGCCGGTTGTTTGATCGGAGCCGAACACGTAAATAATTCATATGAATGTGACATCCCAATCGGG
GCCGGGATCTGCGCTAGTTACCAGACCCAGACTAACTCACCACGGAGAGCCAGAAGTGTAGCCTCCCAG
TCAATAATCGCCTATACAATGTCTTTGGGCGCGGAAAATAGCGTGGCCTACTCCAATAATTCCATAGCC
ATTCCCACTAACTTCACAATTTCCGTCACGACCGAGATCCTGCCGGTGTCAATGACAAAAACCTCTGTG
GACTGCACAATGTATATATGTGGCGACAGTACCGAGTGTTCTAATCTCCTTTTGCAGTATGGGTCCTTC
TGCACACAGCTGAATCGCGCTCTTACTGGTATCGCTGTGGAGCAAGATAAAAACACGCAGGAAGTGTTC
GCTCAAGTGAAGCAGATTTATAAAACCCCTCCAATCAAAGATTTCGGCGGATTTAACTTCTCACAGATT
CTCCCTGATCCAAGTAAACCCTCCAAAAGAAGTTTCATAGAGGACCTCCTGTTCAACAAGGTTACGCTG
GCTGATGCTGGTTTCATCAAGCAGTATGGGGACTGCCTCGGCGACATAGCTGCTCGAGATTTGATCTGC
GCCCAGAAATTCAATGGGCTGACAGTGCTGCCACCTCTCTTGACAGACGAAATGATTGCACAATATACT
AGTGCCCTTTTGGCTGGCACCATCACATCCGGTTGGACTTTTGGTGCGGGAGCCGCACTCCAAATACCC
TTCGCAATGCAGATGGCATACAGATTCAATGGCATTGGCGTCACCCAGAACGTTCTCTACGAAAACCAG
AAACTGATAGCAAATCAGTTTAACAGCGCTATCGGCAAGATCCAGGACTCCCTCTCATCAACTGCCTCA
GCCCTGGGCAAGCTCCAGGATGTGGTAAACCAAAACGCCCAGGCCCTCAACACTCTGGTCAAGCAGCTC
AGTTCTAACTTCGGAGCCATCTCTAGTGTTCTCAATGACATCCTCTCTCGGCTGGACAAGGTGGAAGCA
GAGGTGCAGATCGACCGCCTGATTACTGGACGCCTGCAGAGCCTCCAGACCTACGTAACCCAGCAACTC
ATCCGGGCTGCCGAGATTAGGGCTTCCGCGAACCTCGCCGCCACAAAGATGAGCGAGTGCGTACTTGGA
CAGAGCAAGAGAGTGGATTTTTGTGGCAAGGGGTATCATCTCATGTCCTTCCCTCAGAGCGCTCCTCAC
GGTGTCGTATTTCTCCATGTGACTTATGTCCCCGCCCAAGAGAAAAACTTCACAACCGCTCCTGCTATC
TGCCACGACGGCAAAGCCCATTTTCCTCGGGAGGGAGTGTTCGTCTCTAATGGGACTCACTGGTTTGTT
ACGCAGAGGAACTTCTACGAGCCTCAGATCATCACAACAGATAACACCTTTGTGTCTGGTAACTGTGAC
GTGGTCATCGGCATAGTAAATAACACCGTCTACGACCCACTGCAGCCTGAACTGGATAGCTTTAAGGAA
GAGCTCGATAAGTACTTCAAAAACCACACAAGCCCGGATGTAGATCTTGGAGACATTAGTGGGATTAAC
GCCAGCGTCGTTAACATACAAAAAGAGATTGACCGATTGAACGAAGTTGCCAAAAACCTGAATGAATCC
CTGATCGATCTGCAGGAGCTCGGCAAGTACGAGCAGTATATTAAATGGCCCTGGTACATTTGGCTGGGC
TTCATCGCGGGGCTCATCGCGATTGTGATGGTGACCATTATGCTGTGTTGCATGACCAGTTGCTGTAGC
TGTCTCAAAGGATGCTGCTCATGTGGCAGCTGTTGCAAGTTCGACGAGGATGATTCCGAACCCGTACTG
AAAGGCGTGAAACTCCATTATACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>VC102557 representing YP_009724390
Blue=ORF Red=Cloning site Green=Tag(s)

MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIH
VSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCND
PFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKH
TPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFL
LKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNAT
RFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQ
TGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGV
EGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVL
TESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVP
VAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQ
SIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSF
CTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTL
ADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIP
FAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQL
SSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLG
QSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFV
TQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGIN
ASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCS
CLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_045512
ORF Size 3819 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NC_045512.2, YP_009724390
RefSeq ORF 3819 bp
Locus ID 43740568
MW 141.2 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.