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Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Goat IgG |
| Conjugation | HRP |
| Immunogen | Goat IgG, whole molecule |
Secondary Antibodies
View as table DownloadMouse IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA : 1:50,000 - 1:300,000 Western Blot : 1:10,000 - 1:30,000 Immunohistochemistry: 1:500 - 1:2,500 Other Dilution: User Optimized |
| Conjugation | HRP |
| Immunogen | Mouse IgG whole molecule. |
Goat IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/50,000-1/100,000. Western Blot: 1/5,000-1/20,000. Immunohistochemistry: 1/1,000-1/5,000. Note: HRP Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. |
| Conjugation | HRP |
| Immunogen | Goat IgG whole molecule. |
Peroxidase Conjugated Rabbit Anti-Rat IgG (gamma-chain specific)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Rat IgG |
| Conjugation | HRP |
| Immunogen | Rat IgG, whole molecule |
Monkey IgG (Fab specific) rabbit polyclonal antibody, HRP
| Applications | Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommneded Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/10000. |
| Reactivities | Monkey |
| Conjugation | HRP |
| Immunogen | Purified normal Fab of polyclonal Rhesus Monkey IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgE (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Purified homogenous IgE isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Bovine IgM (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids. Recommneded Dilutions: Histochemistry and Cytochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000. |
| Reactivities | Bovine |
| Conjugation | HRP |
| Immunogen | Purified normal IgM isolated from pooled Bovine serum. Feund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in monkey serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/2000-1/8000. |
| Reactivities | Monkey |
| Conjugation | HRP |
| Immunogen | Purified immunoglobulin IgG, IgA and IgM fractions isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in monkey serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: Histochemical and cytochemical: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000. |
| Reactivities | Monkey |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled Rhesus Monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgM (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions Histochemical and cytochemical Use: 1/100- 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000- 1/5,000. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Purified homogenous IgM isolated from Mouse serum. Immunization with intact and split IgM. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Bovine IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western blot: 1/1,000-1/10,000 or Dot blot), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Bovine |
| Conjugation | HRP |
| Immunogen | Bovine IgG whole molecule. |
Rat IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidas antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/170,000 Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500- 1/2,500. |
| Conjugation | HRP |
| Immunogen | Rat IgG whole molecule |
Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Goat IgG |
| Conjugation | HRP |
| Immunogen | Goat IgG, whole molecule |
Duck IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. In enzyme-immunocytochemical and immunohistochemical staining for the detection of duck IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In nonisotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in duck serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. |
| Reactivities | Duck |
| Conjugation | HRP |
| Immunogen | Purified normal Ig fractions isolated from pooled Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea pig IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Note: This product has been assayed against 1.0 µg of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions: ELISA: 1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. |
| Reactivities | Guinea Pig |
| Conjugation | HRP |
| Immunogen | Guinea Pig IgG whole molecule. |
Mouse Kappa Light Chain rabbit polyclonal antibody, HRP
| Applications | Suitable for immunoblotting (Western or dot blot), ELISA, immuno-peroxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/2,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. |
| Conjugation | HRP |
| Immunogen | Mouse k (kappa) light chain |
Mouse lambda light chain rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/10,000 - 1/50,000 . Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry on paraffin sections: 1/500 - 1/2,500. |
| Conjugation | HRP |
| Immunogen | Mouse lambda light chain |
Peroxidase Conjugated Rabbit Anti-human IgG (gamma-chain specific)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Human IgG |
| Conjugation | HRP |
| Immunogen | Human IgG, whole molecule |
Sheep IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in sheep serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8.000. |
| Reactivities | Sheep |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in sheep serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000. |
| Reactivities | Sheep |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in sheep serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. |
| Reactivities | Sheep |
| Conjugation | HRP |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000. |
| Reactivities | Sheep |
| Conjugation | HRP |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of swine origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in swine serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. |
| Reactivities | Porcine |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in swine serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electrondense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. |
| Reactivities | Porcine |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Bovine IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for immunoblotting (western or dot blot 1:1,000 - 1:10,000), ELISA (1:10,000 - 1:50,000=, immunoperoxidase electron microscopy and immunohistochemistry (1:500 - 1;2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Bovine |
| Conjugation | HRP |
| Immunogen | Bovine IgG F(c) fragment |
Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (1:1,000-10,000), ELISA (1:10,000 - 1:50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1:500-1:2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Bovine |
| Conjugation | HRP |
| Immunogen | Bovine IgG F(ab')2 fragment. |
Feline IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:16,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Cat IgG whole molecule. |
Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: Western blot: 1/1,000-1/10,000. ELISA: 1/10,000-1/50,000. Immunohistochemistry: 1/500-1/2500. |
| Conjugation | HRP |
| Immunogen | Chicken IgY/IgG whole molecule |
Canine IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:40,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Dog IgG F(c) fragment |
Human IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/300,000 Western Blot: 1/5,000-1/25,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Conjugation | HRP |
| Immunogen | Human IgG whole molecule |
Human IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/10,000. Immunohistochemistry: 1/500-2,500. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Human IgG F(ab')2 fragment |
Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, HRP
| Applications | Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western blotting as well as other anti IgG1 antibody based assays. Recommended Dilutions: ELISA: 1/300,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Mouse IgG1 gamma 1 heavy chain |
Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, HRP
| Applications | This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, Western-blot, Immunoprecipitation and more generally immunoassays. Anti-Mouse IgG2a heavy chain antibody is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/90,000 Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. |
| Conjugation | HRP |
| Immunogen | Mouse IgG2a heavy chain |
Porcine IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/150,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. |
| Conjugation | HRP |
| Immunogen | Swine IgG whole molecule |
Canine IgG2 rabbit polyclonal antibody, HRP
| Applications | ELISA: 1:10,000 - 1:100,000. Western Blot: 1:1000 - 1:10,000. Immunohistochemistry on frozen sections: 1:200 - 1:500. |
| Reactivities | Canine |
| Conjugation | HRP |
Peroxidase Conjugated Rabbit Anti-Rat IgG (gamma-chain specific)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Rat IgG |
| Conjugation | HRP |
| Immunogen | Rat IgG, whole molecule |
Canine IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This antibody has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:16,000 to 1:72,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Dog IgG whole molecule |
Equine IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (1/1,000-1/10,000), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/200,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Horse IgG F(c) fragment. |
Equine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot: use at 1/1,000-1/5,000), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry (use at 1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Horse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Horse IgG F(ab')2 fragment. |
Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/50,000-1/100,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. Note: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:9,000 to 1:41,000 is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Human IgG whole molecule. |
Rat IgM (Mu chain specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. |
| Conjugation | HRP |
| Immunogen | Rat IgM mu heavy chain. |
Sheep IgA rabbit polyclonal antibody, HRP
| Applications | ELISA: 1:10,000 - 1:100,000. |
| Reactivities | Sheep |
| Conjugation | HRP |
Goat IgA rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/10,000-1/100,000. |
| Reactivities | Goat |
| Conjugation | HRP |
| Immunogen | Purified goat IgA. |
Goat IgM rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/10,000-1/100,000. |
| Reactivities | Goat |
| Conjugation | HRP |
| Immunogen | Purified Goat IgM |
Donkey IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. Note: This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Donkey IgG whole molecule. |
Chicken IgG / Chicken IgY (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:37,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Chicken IgG F(c) fragment. |
Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Chicken IgG F(ab')2 fragment |
Feline IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/10000 - 1/50000. Western Blot: 1/1000 - 1/10000. Immunohistochemistry: 1/500 - 1/2500. |
| Conjugation | HRP |
| Immunogen | Cat IgG F(c) fragment |
Feline IgG F(ab')2 specific rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:30,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Cat IgG F(ab')2 fragment. |
Canine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Dog IgG F(ab')2 fragment. |