Canine IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Immunogen | Dog IgG F(c) fragment. |
Goat IgG (H+L chain) rabbit polyclonal antibody, AP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/2,000-1/13,000. Western blot: 1/500-1/2,500. Immunohistochemistry: 1/200-1/1,000. |
| Conjugation | AP |
| Immunogen | Goat IgG whole molecule. |
Guinea pig IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Note: This product has been assayed against 1.0 µg of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions: ELISA: 1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. |
| Reactivities | Guinea Pig |
| Conjugation | HRP |
| Immunogen | Guinea Pig IgG whole molecule. |
Human IgG (Fc specific) rabbit polyclonal antibody, FITC
| Applications | This Fluorescein Conjugated antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF MIscroscopy: 1/1000-1/5000. |
| Reactivities | Human |
| Conjugation | FITC |
| Immunogen | Immunogen Human IgG F(c). |
Mouse IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified
| Applications | Immunoprecipitation. Immunodiffusion. Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended dilutions: ELISA: 1/40,000-1/180,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Mouse |
| Immunogen | Mouse IgG F(c) fragment |
Mouse Kappa Light Chain rabbit polyclonal antibody, HRP
| Applications | Suitable for immunoblotting (Western or dot blot), ELISA, immuno-peroxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/2,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. |
| Conjugation | HRP |
| Immunogen | Mouse k (kappa) light chain |
Mouse lambda light chain rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/10,000 - 1/50,000 . Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry on paraffin sections: 1/500 - 1/2,500. |
| Conjugation | HRP |
| Immunogen | Mouse lambda light chain |
Biotin Conjugated Rabbit Anti-Goat IgG
| Applications | WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000 |
| Reactivities | Goat IgG |
| Conjugation | Biotin |
| Immunogen | Goat IgG, whole molecule |
Rabbit Anti-Rat IgG Secondary Antibody
| Applications | WB, IHC, IF/ICC, ELISA |
| Reactivities | Rat IgG |
| Conjugation | Unconjugated |
| Immunogen | Rat IgG, whole molecule |
Biotin Conjugated Rabbit Anti-Rat IgG
| Applications | WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000 |
| Reactivities | Rat IgG |
| Conjugation | Biotin |
| Immunogen | Rat IgG, whole molecule |
Biotin Conjugated Rabbit Anti-human IgG
| Applications | WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000 |
| Reactivities | Human IgG |
| Conjugation | Biotin |
| Immunogen | Human IgG, whole molecule |
Peroxidase Conjugated Rabbit Anti-human IgG (gamma-chain specific)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Human IgG |
| Conjugation | HRP |
| Immunogen | Human IgG, whole molecule |
Porcine IgM (heavy chain) rabbit polyclonal antibody, Purified
| Applications | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10000-1/50000. Immunofluorescence: 1/1000-1/5000. Flow Cytometry: 1/500-1/2500. |
| Reactivities | Porcine |
| Immunogen | Swine IgM mu heavy chain |
Monkey IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. |
| Reactivities | Monkey |
| Conjugation | FITC |
| Immunogen | Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Duck IgG (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Antibody titre: Precipitin titre not less than 1/128 when tested against normal Duck serum in agar block titration. |
| Reactivities | Duck |
| Immunogen | Purified normal Ig fractions isolated from pooled Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Duck IgG (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of duck origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/10-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000. |
| Reactivities | Duck |
| Conjugation | Biotin |
| Immunogen | Purified normal Ig fractions isolated from pooled Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (Fc specific) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used As unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5.000. Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. Can be used In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in sheep serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000. |
| Reactivities | Sheep |
| Conjugation | Biotin |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in sheep serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8.000. |
| Reactivities | Sheep |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000. |
| Reactivities | Sheep |
| Conjugation | Biotin |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Frozen Sections. Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Sheep |
| Conjugation | FITC |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in sheep serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000. |
| Reactivities | Sheep |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in sheep serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/4,000. |
| Reactivities | Sheep |
| Conjugation | Biotin |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in sheep serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. |
| Reactivities | Sheep |
| Conjugation | HRP |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000. |
| Reactivities | Sheep |
| Conjugation | Biotin |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000. |
| Reactivities | Sheep |
| Conjugation | HRP |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of swine origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in swine serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000. |
| Reactivities | Porcine |
| Conjugation | Biotin |
| Immunogen | Purified normal IgG isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of swine origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in swine serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. |
| Reactivities | Porcine |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre: 1/64 when tested against pooled normal Swine serum in agar-block immunodiffusion titration. |
| Reactivities | Porcine |
| Immunogen | Purified normal IgG isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of swine origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. |
| Reactivities | Porcine |
| Conjugation | Biotin |
| Immunogen | Purified normal IgG isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in swine serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electrondense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. |
| Reactivities | Porcine |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Assay Dilutions: FLISA: 1/10,000-1/50,000. IF Microscopy: 1/1,000-5,000. |
| Conjugation | FITC |
| Immunogen | Chicken IgG whole molecule |
Bovine IgG (H+L chain) rabbit polyclonal antibody, AP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product. |
| Reactivities | Bovine |
| Conjugation | AP |
| Immunogen | Bovine IgG whole molecule. |
Bovine IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Bovine |
| Immunogen | Bovine IgG whole molecule. |
Bovine IgG (Fc specific) rabbit polyclonal antibody, AP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product. |
| Reactivities | Bovine |
| Conjugation | AP |
| Immunogen | Bovine IgG F(c) fragment. |
Bovine IgG (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Note: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/15,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions: ELISA: 1/20,000-1/100,000 Western Blot: 1/2,000-1/10,000 Immunohistochemistry: 1/1,000-1/5,000 |
| Reactivities | Bovine |
| Conjugation | Biotin |
| Immunogen | Bovine IgG F(c) fragment. |
Bovine IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for immunoblotting (western or dot blot 1:1,000 - 1:10,000), ELISA (1:10,000 - 1:50,000=, immunoperoxidase electron microscopy and immunohistochemistry (1:500 - 1;2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Bovine |
| Conjugation | HRP |
| Immunogen | Bovine IgG F(c) fragment |
Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, AP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,600 to 1:11,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Bovine |
| Conjugation | AP |
| Immunogen | Bovine IgG F(ab')2 fragment. |
Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, Biotin
| Applications | Immunoblotting. ELISA. Immunohistochemistry. Immunofluorescence. |
| Reactivities | Bovine |
| Conjugation | Biotin |
| Immunogen | Bovine IgG F(ab’)2 fragment |
Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (1:1,000-10,000), ELISA (1:10,000 - 1:50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1:500-1:2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Bovine |
| Conjugation | HRP |
| Immunogen | Bovine IgG F(ab')2 fragment. |
Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. |
| Reactivities | Bovine |
| Immunogen | Bovine IgG F(ab')2 fragment. |
Feline IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:16,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Cat IgG whole molecule. |
Human IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended dilutions: ELISA: 1:10,000-1:50,000. Western Blot: 1:1,000-1:5,000. Immunohistochemistry: 1:500-1:3,000. |
| Immunogen | Human IgG F(c) fragment |
Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/350,000. Western Blot: 1/3,000-1/15,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Immunogen | Human IgG F(ab')2 fragment. |
Human IgA (alpha chain) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended dilutions: ELISA: 1/5,000-1/20,000. Western blot: 1/500-1/2,000. IHC: 1/250-1:1,000 |
| Reactivities | Human |
| Immunogen | Human IgA alpha heavy chain. |
FITC Conjugated Rabbit Anti-Rat IgG
| Applications | FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64 |
| Reactivities | Rat IgG |
| Conjugation | FITC |
| Immunogen | Rat IgG, whole molecule |
Rat IgG2b rabbit polyclonal antibody, Purified
| Applications | ELISA: 1µg/ml - 10µg/ml. Western Blot. Flow Cytometry. |
| Reactivities | Rat |
Rat IgG2c rabbit polyclonal antibody, Purified
| Applications | ELISA: 1µg/ml - 10µg/ml. Western Blot. Flow Cytometry. |
| Reactivities | Human, Rat |
Bovine IgG1 (subclass specific) rabbit polyclonal antibody, Serum
| Applications | Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG1 in bovine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of bovine IgG1 from serum or plasma. Antibody titre: Precipitin titre not less then 1/16 when tested against pooled normal bovine serum in agar block immunodiffusion titration. Recommended Dilutions: Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
| Reactivities | Bovine |
| Immunogen | Pools of purified normal IgG1 isolated from pooled Bovine Serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Germany
Japan
United Kingdom
China
