Secondary Antibodies

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Mouse IgA (+IgG +IgM) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/500-1/3,000 for Western blot), ELISA (1/100,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/250-1/1,500) as well as other Peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Conjugation HRP
Immunogen Mouse IgG, IgA and IgM whole molecules.

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Mouse IgG whole molecule.

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, FITC

Applications This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Immunofluorescence: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Conjugation FITC
Immunogen Mouse IgG2a heavy chain.

Rat IgG F(ab)2 specific rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Immunogen Rat IgG F(ab')2 fragment.

Rat IgM (Mu chain specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Rat IgM mu heavy chain.

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/150,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/10,000.
Conjugation Biotin
Immunogen Rat IgG, IgA and IgM whole molecules.

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:2,000-1:10,000.
Western Blot: 1:500-1:2,500.
Immunohistochemistry: 1:200-1:1,000.
Conjugation Texas Red
Immunogen Rat IgG, IgA and IgM whole molecules.

Sheep IgG F(c) specific(adsorbed) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity..
Immunogen Sheep IgG F(c) fragment.

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Immunogen Swine IgG F(ab')2 fragment.

Human IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1/100-1/250) and Flow Cytometry or FACS analysis (1/100-1/250) as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
Conjugation PE
Immunogen Human IgG F(c) fragment.

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/5,000 of the reconstitution concentration is suggested for this product. 
Recommended Dilutions: 
ELISA: 1/2000-1/10000. 
Western Blot: 1/500-1/2500. 
Immunochemistry: 1/200-1/1000.
Reactivities Mouse
Conjugation AP
Immunogen Mouse IgG F(c) fragment.

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin
Immunogen Mouse IgG F(c) fragment.

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Applications
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.  
Recommended Dilutions:
FLISA: 1/10,000-1/50,000. 
IF Microscopy: 1/1,000-1/5,000. 
Flow Cytometry: 1/500-1/2,500. 

 
Reactivities Mouse
Conjugation FITC
Immunogen Mouse IgG F(c) fragment.

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP

Applications ELISA: 1/20000. 
Western Blot: 1/500-1/2000.
Immunohistochemistry: 1/250-1/1000.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG F(c) fragment.

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. 
Recommended Dilutions:
ELISA: 1/10,000. 
Western Blot : 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Immunogen Mouse IgG F(c) fragment.

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Texas Red

Applications This product is designed for Immunofluorescence Microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000. 
Immunofluorescence: 1/1,000-1/5,000. 
Flow Cytometry: 1/500-1/2,500.
Reactivities Mouse
Conjugation Texas Red
Immunogen Mouse IgG F(c) fragment.

Mouse IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1/100-1/250) and Flow Cytometry (1/100-1/250) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.
Conjugation PE
Immunogen Mouse IgG F(ab')2 fragment.

Mouse IgM (Mu chain specific), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. 
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Mouse IgM µ heavy chain

Mouse IgA (H+L chain), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000-1/5,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Mouse IgG, IgA and IgM whole molecules.

Rabbit IgG (H+L chain) F(ab)2 Fragment goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA:  1/1000 - 1/50,000
IF Microscopy:  1/1000 - 1/5000
Conjugation FITC
Immunogen Rabbit IgG whole molecule.

Rabbit IgG (F(ab)2 specific adsorbed F(ab)2 Fragment) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer andspecificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Rabbit IgG F(ab')2 fragment.

Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA:  1:10,000/1:50,000
IF Microscopy:  1:1000/1:5000
Conjugation FITC
Immunogen Rat IgG whole molecule.

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1/100-1/200) and Flow Cytometry (1/100-1/200) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Conjugation PE
Immunogen Goat IgG whole molecule

Bovine IgG (H+L chain) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Fow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation Texas Red
Immunogen Bovine IgG whole molecule.

Mouse IgM (Mu chain specific), adsorbed goat polyclonal antibody, HRP

Applications ELISA (1/1000).
Immunohistochemistry on Frozen and Paraffin Tissues (1/50).
Reactivities Mouse
Conjugation HRP
Immunogen Monoclonal Mouse IgM

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Applications ELISA: 1/1000.
Immunohistochemistry on Frozen Sections: 1/50.
Immunohistochemistry on Paraffin Sections.
Reactivities Rat
Conjugation HRP
Immunogen Rat IgG

Hamster IgG (adsorbed) goat polyclonal antibody, HRP

Applications ELISA: 1/1000 - 1/5000.
Reactivities Hamster
Conjugation HRP
Immunogen Highly purified Hamster IgG

Mouse IgG (Rat adsorbed) goat polyclonal antibody, Aff - Purified

Applications ELISA.
Flow Cytometry.
Reactivities Mouse
Immunogen Mouse IgG

Bovine IgG sheep polyclonal antibody, AP

Applications

ELISA: 1/5000-1/50000.
Western Blot: 1/5000-1/50000.

Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgG.

Bovine IgG sheep polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections: 1/200-1/2,000.
Reactivities Bovine
Conjugation FITC
Immunogen Purified Bovine IgG.

Bovine IgG sheep polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000. 
Western Blot.
 
Reactivities Bovine
Conjugation HRP
Immunogen Purified Bovine IgG.

Bovine IgG sheep polyclonal antibody, Purified

Applications ELISA: 1/100-1/10,000.
Immunodiffusion.
Reactivities Bovine
Immunogen Purified Bovine IgG.

Bovine IgM sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000. 
Western Blot: 1/5000-1/50000.
Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgM.

Bovine IgM sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on frozen sections: 1/200-1/2000.
Reactivities Bovine
Conjugation FITC
Immunogen Purified Bovine IgM

Bovine IgM sheep polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Immunodiffusion.
Reactivities Bovine
Immunogen Purified Bovine IgM

Bovine IgG1 sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20 - 1/100.
Immunohistochemistry on frozen sections: 1/20 - 1/100.
Reactivities Bovine
Conjugation FITC
Immunogen Purified bovine IgG1

Bovine IgG2 sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20 - 1/100.
Immunohistochemistry on frozen sections: 1/20 - 1/100.
Reactivities Bovine
Conjugation FITC
Immunogen Purified bovine IgG2

Bovine IgG2 sheep polyclonal antibody, Aff - Purified

Applications ELISA: 1/100 - 1/10000.
Immunodiffusion.
Reactivities Bovine
Immunogen Purified bovine IgG2

Canine IgA goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1/200-1/2000.
Reactivities Canine
Conjugation FITC
Immunogen Purified Canine IgA.

Canine IgA goat polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:80,000.
Immunhistochemistry on frozen sections: 1:200 - 1:500.
Reactivities Canine
Conjugation HRP
Immunogen Purified canine IgA

Canine IgM goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections:
1/200-1/2000.
Reactivities Canine
Conjugation FITC
Immunogen Purified Canine IgM.

Canine IgM goat polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Canine
Conjugation HRP
Immunogen Purified Canine IgM

Equine IgA goat polyclonal antibody, Purified

Applications ELISA: 1/100 - 1/1000.
Reactivities Equine
Immunogen Purified equine IgA.

Equine IgG goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on Frozen Sections: 1/20-1/100.
Reactivities Equine
Conjugation FITC
Immunogen Purified equine IgG (T).

Equine IgG goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Immunodiffusion.
Reactivities Equine
Immunogen Purified Equine IgG (T).

Equine IgM goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on Frozen Sections: 1/100-1/1000.
Reactivities Equine
Conjugation FITC
Immunogen Purified equine IgM

Equine IgM goat polyclonal antibody, Aff - Purified

Applications ELISA.
Immunodiffusion.
Reactivities Equine
Immunogen Purified equine IgM.

Feline IgA goat polyclonal antibody, Aff - Purified

Applications ELISA.
Immunodiffusion.
Reactivities Feline
Immunogen Purified feline IgA.

Feline IgG (Fc specific) goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections: 1/200-1/2,000.
Reactivities Feline
Conjugation FITC
Immunogen Purified Feline IgG Fc fragment.

Feline IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/10,00.
Immunodiffusion.
Reactivities Feline
Immunogen Purified Feline IgG Fc fragment.