Secondary Antibodies

Download

Sheep IgG F(ab)2 specific (adsorbed) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Sheep IgG F(ab')2 fragment.

Porcine IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Swine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:4,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Swine IgG whole molecule

Porcine IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Swine IgG in a standard capture ELISA using Peroxidase conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Swine IgG whole molecule.

Porcine IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Swine IgG whole molecule.

Porcine IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1:20,000-1:100,000.
Western Blot: 1:2,000-1:10,000.
Immunohistochemistry: 1:1,000-1:5,000.
Immunogen Swine IgG whole molecule

Porcine IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Swine IgG whole molecule.

Porcine IgG (H+L chain) rabbit polyclonal antibody, Texas Red

Applications FLISA: 1/10,000 - 1/50000.
Immunofluorescence: 1/1000 - 1/5000.
Flow Cytometry: 1/500 - 1/2500.
Conjugation Texas Red
Immunogen Swine IgG whole molecule

Porcine IgG F(c) specific rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:500 - 1:2,500.
Western Blot: 1:200 - 1:2,000.
Immunohistochemistry: 1:200 - 1:1,000.
Conjugation AP
Immunogen Swine IgG F(c) fragment.

Porcine IgG F(c) specific rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Swine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Swine IgG F(c) fragment.

Porcine IgG F(c) specific rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Swine IgG F(c) fragment.

Porcine IgG F(c) specific rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommendeed Dilutions: This product has been assayed against 1.0 µg of Swine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:6,500 to 1:27,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Swine IgG F(c) fragment.

Porcine IgG F(c) specific rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Immunogen Swine IgG F(c) fragment.

Porcine IgG F(c) specific rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Optimal titers for applications should be determined by the researcher.
FLISA:  1/10,000 - 1/50,000
IF Microscopy:  1/1000 - 1/5000
Conjugation TRITC
Immunogen Swine IgG F(c) fragment.

Porcine IgG F(c) specific rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Swine IgG F(c) fragment.

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:1,000-1:3,000.
Western Blot: 1:500-1:2,000.
Immunohistochemistry: 1:200-1:1,000.
Conjugation AP
Immunogen Swine IgG F(ab')2 fragment.

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Swine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:30,000 to 1:120,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Swine IgG F(ab')2 fragment.

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilution(s):
Flow Cytometry: 1:2,000-1:10,000.
IF Microscopy: 1:500-1:2,500.
Conjugation FITC
Immunogen Swine IgG F(ab')2 fragment.

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Swine IgG F(ab')2 in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Swine IgG F(ab')2 fragment.

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Swine IgG F(ab')2 fragment.

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, Texas Red

Applications FLISA: 1/10000 - 1/50000.
Immunofluorescence: 1/1000 - 1/5000.
Flow Cytometry: 1/500 - 1/2500.
Conjugation Texas Red
Immunogen Swine IgG F(ab')2 fragment

Goat IgG (H+L chain), Fab Fragment, adsorbed donkey polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:8,000 to 1:36,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Goat IgG whole molecule.

Goat IgG (H+L chain), Fab Fragment, adsorbed donkey polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
Flow cytometry 1:2,000 - 1:10,000
Immunofluorescence microscopy 1:500 - 1:2,500.
Conjugation FITC
Immunogen Goat IgG whole molecule.

Goat IgG (H+L chain), Fab Fragment, adsorbed donkey polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:20,000-1:100,000.
Western Blot: 1:2,000-1:10,000.
Conjugation HRP
Immunogen Goat IgG whole molecule.

Goat IgG (H+L chain), Fab Fragment, adsorbed donkey polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions for ELISA: 1:10,000-1:50,000.
Immunogen Goat IgG whole molecule.

Goat IgG (H+L chain), Fab Fragment, adsorbed donkey polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation TRITC
Immunogen Goat IgG whole molecule.

Goat IgG (H+L chain), Fab Fragment, adsorbed donkey polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation Texas Red
Immunogen Goat IgG whole molecule.

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000-1/50,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Human IgG whole molecule.

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
IF Microscopy: 1/1,000-1/5,000.
Conjugation FITC
Immunogen Human IgG whole molecule

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Note: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions:
ELISA: 1/300,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Human IgG whole molecule.

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/40,000.
IHC: 1/1,000-1/5,000
Western Blot: 1/2,000-1/10,000.
Immunogen Human IgG whole molecule

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, TRITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation TRITC
Immunogen Human IgG whole molecule

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation Texas Red
Immunogen Human IgG, whole molecule.

Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA,
Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:30,000 to 1:140,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Mouse IgG whole molecule.

Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, FITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
Flow cytometry 1:2,000 - 1:10,000
Immunofluorescence microscopy 1:500 - 1:2,500.
Conjugation FITC
Immunogen Mouse IgG whole molecule

Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/10,000-1:100,000.
Westrn Blot: 1/2,000-1/20,000.
Immunohistochemistry: 1:500-1:2,500.
Conjugation HRP
Immunogen Mouse IgG whole molecule.

Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgG whole molecule.

Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation Texas Red
Immunogen Mouse IgG whole molecule.

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, Biotin

Applications Suitable for Western Blot, ELISA,
Immunohistochemistry, Immunomicroscopy, as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:250,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Rabbit IgG whole molecule.

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immonoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremly low background levels, absence of F(c) mediated binding, lot-to-lot consitency, high titer and specificy.
ELISA: 1:82,000.
Western blot: 1:1,000 - 1:5,000.
Immunohistochemistry: 1:500 - 1:2,500.
Conjugation HRP
Immunogen Rabbit IgG whole molecule

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilution for ELISA: 1:4,000 - 1:20,000.
Immunogen Rabbit IgG whole molecule.

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, TRITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Rabbit IgG whole molecule

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Rabbit IgG whole molecule.

Rat IgG (H+L chain), Fab Fragment goat polyclonal antibody, Biotin

Applications Immunoblotting
ELISA
Immunohistochemistry
Immunomicroscopy
as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:110,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Rat IgG whole molecule.

Rat IgG (H+L chain), Fab Fragment goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation FITC
Immunogen Rat IgG whole molecule.

Rat IgG (H+L chain), Fab Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:10,000 - 1:40,000.
Western Blot: 1:1,000 - 1:4,000.
Conjugation HRP
Immunogen Rat IgG whole molecule.

Rat IgG (H+L chain), Fab Fragment goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA: 1:5,000-1:20,000.
Immunogen Rat IgG whole molecule.

Rat IgG (H+L chain), Fab Fragment goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Rat IgG whole molecule.

Rat IgG (H+L chain), Fab Fragment goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Rat IgG whole molecule.

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:10,000 - 1:40,000.
Western Blot: 1:500 - 1:5,000.
Immunohistochemistry: 1:200 - 1:2,000.
Conjugation AP
Immunogen Goat IgG whole molecule.

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:40,000 to 1:200,000 is suggested for this product.
The following general recommendations are suggested as starting dilutions for:
Immunoblotting: 1:10,000,
Enzyme Immunohisto-chemistry on tissue sections: 1:500-1:5,000.
Flow cytometry and fluorescence Immunohisto/cytochemistry: 1:200-1:1,000.
Conjugation Biotin
Immunogen Goat IgG whole molecule.