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Goat Anti-Rabbit IgG Secondary Antibody
| Applications | WB, IHC, IF/ICC, ELISA |
| Reactivities | Rabbit IgG |
| Conjugation | Unconjugated |
Antibodies
View as table DownloadUSD 260.00
5 Days
Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), HRP, Purified
| Applications | ELISA: 1/4000-1/8000 (See also Ref.2-9). Applications Reported in Literature: Flow Cytometry (Ref.15,18,19). Immunohistochemistry on Frozen Sections (Ref.10). Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11). Immunocytochemistry (Ref.12). Immunoblotting (Ref.3,13-16). Microarray (Ref.17). Multiplex (Ref.2,20-22). Purification (Ref.15). |
| Reactivities | Human |
| Conjugation | HRP |
USD 300.00
5 Days
Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), AP, Purified
| Applications | ELISA: 1/1000-1/2000 (See also Ref.2-9). FLISA. Applications Reported in Literature: Flow Cytometry (Ref.15,18,19). Immunohistochemistry on Frozen Sections (Ref.10). Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11). Immunocytochemistry (Ref.12). Immunoblotting (Ref.3,13-16). Microarray (Ref.17). Multiplex (Ref.2,20-22). Purification (Ref.15). |
| Reactivities | Human |
| Conjugation | AP |
IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified
| Applications | ELISA: 1/1000-1/2000 (2-9). Immunohistochemistry on Frozen Sections: reported in literature (5,7,10,11). Immunohistochemistry on Paraffin Sections: reported in literature (10). Flow Cytometry: reported in literature (15, 18, 19). Immunocytochemistry: reported in literature (12). Western Blot: reported in literature (3,13-16). |
| Reactivities | Human |
| Conjugation | AP |
Goat Anti-Mouse IgG Secondary Antibody
| Applications | WB, IHC, IF/ICC, ELISA |
| Reactivities | Mouse IgG |
| Conjugation | Unconjugated |
Biotin Conjugated Goat Anti-Rabbit IgG
| Applications | WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000 |
| Reactivities | Rabbit IgG |
| Conjugation | Biotin |
Rabbit Anti-Mouse IgG Secondary Antibody
| Applications | WB, IHC, IF/ICC, ELISA |
| Reactivities | Mouse IgG |
| Conjugation | Unconjugated |
USD 680.00
2 Weeks
Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Biotin
| Applications | To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. General Recommended Dilutions: Immunocytochemistry. Immunohistochemistry (Paraffin). Histochemical Use: 1/10-1/40. ELISA: 1/100-1/2000. Western blot: 1/200-1/4000. |
| Reactivities | Human |
| Conjugation | Biotin |
Rabbit IgG (H+L chain) goat polyclonal antibody, HRP, Lyophilized
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/150,000-1/250,000. Immunohistochemistry: 1/500-1/2,500. Western blot: 1/10,000-1/40,000. |
| Reactivities | Rabbit |
| Conjugation | HRP |
Biotin Conjugated Goat Anti-Mouse IgG
| Applications | WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000 |
| Reactivities | Mouse IgG |
| Conjugation | Biotin |
Rabbit Anti-Goat IgG Secondary Antibody
| Applications | WB, IHC, IF/ICC, ELISA |
| Reactivities | Goat IgG |
| Conjugation | Unconjugated |
Rabbit Monoclonal Anti-Human IgG4 Antibody, Clone RM120
| Applications | ELISA: 50ng/well - 200ng/well for Capture;, 0.05ug/mL - 0.2ug/mL for Detection;, , WB: 0.5 ug/mL - 2 ug/mL;, ICC: 1 ug/mL-10ug/mL;, IHC: 1 ug/mL-10ug/mL |
| Reactivities | Human IgG4 |
| Conjugation | Unconjugated |
Biotin Conjugated Goat Anti-Rabbit IgG
| Applications | WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000 |
| Reactivities | Rabbit IgG |
| Conjugation | Biotin |
Rabbit IgG (H+L chain) goat polyclonal antibody, Aff - Purified
| Applications | Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: IF Microscopy. ELISA: 1/10,000-1/60,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2500. Immunoprecipitation. |
| Reactivities | Rabbit |
Monkey IgG (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used: • In direct staining of cytoplasmic IgG in fixed Monkey cells and tissue substrates. • To identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases. • To identify a specific antigen or immune complex using a reference antibody of Monkey in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilution: 1/20-1/80. |
| Reactivities | Monkey |
| Conjugation | FITC |
Mouse IgG (H+L chain) sheep polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Mouse |
Goat Monoclonal Anti-Mouse IgG Fc Antibody, Clone RMG06
| Applications | ELISA: 0.05ug/mL - 1ug/mL;, ICC, IHC: 0.5ug/mL - 2ug/mL;, WB: 0.1ug/mL - 0.5ug/mL |
| Reactivities | Mouse IgG |
| Conjugation | Unconjugated |
USD 1,340.00
2 Weeks
Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, HRP, Purified
| Applications | Immunoassay. ELISA: good binding on plastic. Immunohistochemistry on Frozen Sections. |
| Reactivities | Mouse |
| Conjugation | HRP |
Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, Purified
| Applications | ELISA: 5 µg/ml. This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent and purified Rat IgA (Cat.-No SA013) as antigen. Immunofluorescence. Immunohistochemistry on Frozen Sections. |
| Reactivities | Rat |
Human IgG (Fc specific) goat polyclonal antibody, HRP
| Applications | ELISA: 1/3000. Immunohistochemistry on Frozen Sections. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgG (Fc specific) goat polyclonal antibody, Aff - Purified
| Applications | ELISA: 1-5 µg/ml. Immunohistochemistry on Frozen Sections. |
| Reactivities | Human |
Human IgG3 sheep polyclonal antibody, HRP
| Applications | ELISA. Immunohistochemistry on Frozen Sections: 1/100-1/200. |
| Reactivities | Human |
| Conjugation | HRP |
Mouse IgG (Fc specific) goat polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Mouse |
Biotin Conjugated Goat Anti-Mouse IgM
| Applications | WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000 |
| Reactivities | Mouse IgM |
| Conjugation | Biotin |
USD 220.00
2 Weeks
Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified
| Applications | Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C. SDS-PAGE (12% separating gel). Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min. Strongly reacts with Bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry on Frozen Sections. |
| Reactivities | Bovine, Sheep |
Biotin Conjugated Rabbit Anti-Goat IgG
| Applications | WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000 |
| Reactivities | Goat IgG |
| Conjugation | Biotin |
Rabbit Monoclonal Anti-Mouse IgG1 antibody, clone RM106
| Applications | ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB: 0.1ug/mL-0.5ug/mL |
| Reactivities | Mouse IgG1 |
| Conjugation | Unconjugated |
Rabbit Monoclonal Anti-Human IgG1 Antibody, Clone RM117
| Applications | ELISA: 50ng/well - 200ng/well for Capture;, 0.05ug/mL - 0.2ug/mL for Detection;, ICC: 0.5ug/mL-2ug/mL, IHC: 0.5ug/mL-2ug/mL |
| Reactivities | Human IgG1 |
| Conjugation | Unconjugated |
Mouse IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA : 1:50,000 - 1:300,000 Western Blot : 1:10,000 - 1:30,000 Immunohistochemistry: 1:500 - 1:2,500 Other Dilution: User Optimized |
| Conjugation | HRP |
Mouse IgG (H+L chain) sheep polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/200,000 Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. |
| Reactivities | Mouse |
| Conjugation | HRP |
IgG (Immunglobulin G, light chain), mouse anti porcine, clone PHE425
| Applications | Tested for immunohistochemistry (IHC), ELISA and Western Blot, other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.1µg/ml (1:2000) IHC, paraffin sections: 0.25µg/ml (1:800); Proteinase K pretreatment for antigen retrieval is recommended. Western Blot: 0.05 – 0.5µg/ml. ELISA: 20 - 50ng/ml (EC50 of 1ng/ml illustrated below) Optimal dilutions should be determined by the end user. |
| Reactivities | Porcine |
| Conjugation | Unconjugated |
Mouse IgD (Fc specific) goat polyclonal antibody, Biotin
| Applications | In Immunocytochemical and Immunohistochemical staining of IgD at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgD antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgD isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgD in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/200-1/1000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining to identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000 - 1/20000. |
| Reactivities | Rabbit |
| Conjugation | Biotin |
Goat IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/50,000-1/100,000. Western Blot: 1/5,000-1/20,000. Immunohistochemistry: 1/1,000-1/5,000. Note: HRP Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. |
| Conjugation | HRP |
Rabbit Anti-Human IgG Secondary Antibody
| Applications | WB, IHC, IF/ICC, ELISA |
| Reactivities | Human IgG |
| Conjugation | Unconjugated |
Rabbit Monoclonal Anti-Human IgM antibody, clone RM121
| Applications | ELISA: 50ng/well – 200ng/well (for Capture);0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL |
| Reactivities | Human IgM |
| Conjugation | Unconjugated |
Rabbit Monoclonal Anti-Human IgA antibody, clone RM128
| Applications | ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL |
| Reactivities | Human IgA |
| Conjugation | Unconjugated |
Goat Monoclonal Anti-Mouse IgG Fab Antibody, Clone RMG05
| Applications | ELISA: 0.05ug/mL - 1ug/mL;, ICC, IHC: 0.5ug/mL - 2ug/mL;, WB: 0.1ug/mL - 0.5ug/mL |
| Reactivities | Mouse IgG |
| Conjugation | Unconjugated |
Monkey IgG (Fab specific) rabbit polyclonal antibody, FITC
| Applications | Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. |
| Reactivities | Monkey |
| Conjugation | FITC |
Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, HRP
| Applications | ELISA: 1/100,000. Western blot: 1/500-1/3,000. Immunohistochemistry: 1/500-1/1,500. |
| Conjugation | HRP |
Human IgA (secretory) mouse monoclonal antibody, clone SC-05, Purified
| Applications | Western blot. Immunoprecipitation. Immunohistochemistry on Paraffin Sections. Immunohistochemistry on Frozen Sections. Examples of positive Human tissues: Secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland. |
| Reactivities | Human |
Goat Monoclonal Anti-Mouse IgG Antibody, Clone RMG07
| Applications | ELISA: 0.05ug/mL - 1ug/mL;, ICC, IHC: 0.5ug/mL - 2ug/mL;, WB: 0.1ug/mL - 0.5ug/mL |
| Reactivities | Mouse IgG |
| Conjugation | Unconjugated |
USD 230.00
3 Days
Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, Purified
| Applications | Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/106 cells. ELISA. Immunohistochemistry on Frozen Sections. Western blotting. Dot- and Slot-Immunoblotting. Immunocytochemistry. |
| Reactivities | Human |
Mouse IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. depending on the method used. |
| Reactivities | Mouse |
| Conjugation | HRP |
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP
| Applications | ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids). Immunocytochemistry. Immunohistochemistry. Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties. Dot blot. Western blot. General Recommended Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen. |
| Reactivities | Rabbit |
| Conjugation | HRP |
Mouse IgG (Fc specific) goat polyclonal antibody, HRP
| Applications | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000 Western blot: 1/1,000-1/5,000 Flow Cytometry: 1/500-1/2,500 |
| Reactivities | Mouse |
| Conjugation | HRP |
Canine IgG2 rabbit polyclonal antibody, Purified
| Applications | ELISA: 1:100 - 1:10,000. Western Blot: 1:1000 - 1:10,000. Immunohistochemistry on frozen sections: 1:200 - 1:2000. |
| Reactivities | Canine |
Mouse IgG1 goat polyclonal antibody, AP
| Applications | ELISA: 1/2000-1/4000. Western Blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. |
| Reactivities | Mouse |
| Conjugation | AP |
Human IgA (Secretory component) goat polyclonal antibody, Biotin
| Applications | This antibody can be used In Immunocytochemical and Immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in Human body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted and the optimum working dilution should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500 ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000. |
| Reactivities | Human |
| Conjugation | Biotin |
Human IgA (Secretory component) goat polyclonal antibody, HRP
| Applications | In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in human milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/6000. |
| Reactivities | Human |
| Conjugation | HRP |
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