E1B 19K (AC_000006) Virus Tagged ORF Clone

CAT#: VC100405

Myc-DDK-tagged ORF clone of viral ORF for E1B 19K [Human adenovirus D], codon optimized for human cell expression, AP_000142

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B 19K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100405 represents NCBI reference of AP_000142 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTGTGGACAATCCTGGCCGACTTTTCAAAAACACGGCGGCTGGTTGAAGACAGTTCTGACGGAT
  GCTCTGGATTTTGGAGGCATTGGTTTGGGACCCCACTCAGTCGACTCGTTTATACCGTTAAGAAAGACTA
  CAACGAAGAGTTCGAGAATCTCTTTGCCGACTGCAGCGGTTTGCTGGACTCACTCAATCTCGGGCATCAA
  TCCCTGTTCCAAGAAAGGGTTCTCCATTCCCTCGACTTCAGTAGCCCAGGCCGGACCACTGCCGGAGTGG
  CCTTCGTCGTTTTTCTCGTGGACAAATGGTCTCAGAATACACAGCTGTCTCGAGGCTATATACTTGACTT
  CGCCGCAATGCACCTGTGGCGAGCCTGGGTGCGGCAGCGCGGACAGCGGATTCTGAATTATTGGCTCCTT
  CAGCCTGCCGCCCCCGGTCTCCTGAGACTGCATAGGCAAACATCAATGTTGGAAGAAGAGATGAGACAGG
  CGATGGACGAAAACCCCCGATCTGGACTCGACCCCCCCTCCGAAGAAGAACTCGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100405 representing AP_000142
  Red=Cloning sites Green=Tags
  
  MDVWTILADFSKTRRLVEDSSDGCSGFWRHWFGTPLSRLVYTVKKDYNEEFENLFADCSGLLDSLNLGHQ
  SLFQERVLHSLDFSSPGRTTAGVAFVVFLVDKWSQNTQLSRGYILDFAAMHLWRAWVRQRGQRILNYWLL
  QPAAPGLLRLHRQTSMLEEEMRQAMDENPRSGLDPPSEEELD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000006
• ORF Size: 546 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000006.1, AP_000142
• RefSeq ORF: 546
• MW: 21.1 kDa