E3 14.7K (AC_000006) Virus Tagged ORF Clone

CAT#: VC100418

Myc-DDK-tagged ORF clone of viral ORF for E3 147K [Human adenovirus D], codon optimized for human cell expression, AP_000155

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 14.7K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100418 represents NCBI reference of AP_000155 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGACCATCACCTGGATCTCGAGATGGATGGACAAGCCAGTGAGCAACGAATCCTTCAGCTCCGCG
  TGCGACAGCAACAGGAGAGGGCTGCCAAAGAGCTGCTGGATGCAATCAACATTCACCAGTGTAAGAAAGG
  GATTTTTTGCTTGGTGAAACAGGCTAAGATTACCTATGAGCTCGTGAGTAACGGAAAACAGCACCGACTT
  ACATACGAAATGCCACAACAGAAGCAAAAGTTCACATGCATGGTCGGAGTGAACCCCATCGTCATCACCC
  AGCAGTCTGGCGAGACCAACGGGTGTATCCATTGCTCTTGTGAGTCTCCAGAGTGTATATACTCACTGCT
  GAAAACTCTCTGCGGGCTCAGGGACCTGCTTCCCATGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100418 representing AP_000155
  Red=Cloning sites Green=Tags
  
  MTDHHLDLEMDGQASEQRILQLRVRQQQERAAKELLDAINIHQCKKGIFCLVKQAKITYELVSNGKQHRL
  TYEMPQQKQKFTCMVGVNPIVITQQSGETNGCIHCSCESPECIYSLLKTLCGLRDLLPMN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000006
• ORF Size: 390 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000006.1, AP_000155
• RefSeq ORF: 390
• MW: 14.8 kDa