pVI (AC_000006) Virus Tagged ORF Clone

CAT#: VC100412

Myc-DDK-tagged ORF clone of viral ORF for pVI [Human adenovirus D], codon optimized for human cell expression, AP_000149

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100412 represents NCBI reference of AP_000149 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACATTAATTTCGCCTCTCTTGCTCCAAGGCACGGGACCCGGCCTTTTATGGGGACTTGGAACG
  AAATTGGAACTTCCCAGCTGAATGGTGGTGCTTTTAATTGGAGTAGTGTGTGGTCTGGCCTTAAGAACTT
  CGGTAGTACCTTGCGCACATACGGAAACAAAGCGTGGAATTCTTCCACGGGCCAACTGCTGCGCGAGAAA
  CTGAAGGATCAGAATTTTCAGCAGAAAGTCGTTGATGGCCTTGCAAGTGGCATCAACGGAGTAGTAGATA
  TCGCCAATCAGGCCGTTCAGAGGGAGATCAATAGCCGCCTGGATCCAAGGCCCCCAACAGTTGTGGAAAT
  GGAGGATGCTACATTGCCTCCACCCAAAGGCGAAAAAAGGCCTCGACCTGACGCCGAGGAAACTATTCTC
  CAAGTGGACGAACCCCCTAGCTACGAGGAGGCCGTGAAGGCAGGGATGCCGACCACCCGGATCATAGCCC
  CCCTGGCTACAGGCGTGATGAAGCCCGCTACGCTGGATCTGCCTCCTCCACCTACACCTGCCCCCCCTAA
  GGCCGCACCAGTAGTACAGCCCCCGCCCGTAGCGACTGCCGTGAGAAGAGTCCCCGCTAGGAGACAGGCT
  CAGAATTGGCAGTCTACCCTGCATTCCATCGTTGGACTGGGGGTGAAGTCACTGAAGAGGCGACGGTGTT
  AC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100412 representing AP_000149
  Red=Cloning sites Green=Tags
  
  MEDINFASLAPRHGTRPFMGTWNEIGTSQLNGGAFNWSSVWSGLKNFGSTLRTYGNKAWNSSTGQLLREK
  LKDQNFQQKVVDGLASGINGVVDIANQAVQREINSRLDPRPPTVVEMEDATLPPPKGEKRPRPDAEETIL
  QVDEPPSYEEAVKAGMPTTRIIAPLATGVMKPATLDLPPPPTPAPPKAAPVVQPPPVATAVRRVPARRQA
  QNWQSTLHSIVGLGVKSLKRRRCY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000006
• ORF Size: 702 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000006.1, AP_000149
• RefSeq ORF: 702
• MW: 25.5 kDa