III (AC_000006) Virus Tagged ORF Clone

CAT#: VC100410

Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus D], codon optimized for human cell expression, AP_000147

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: III
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100410 represents NCBI reference of AP_000147 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCGCGCTGTTGTCAGCTCCTCCCCGCCACCCTCTTACGAAAGCGTCATGGCTCAGGCAACACTTG
  AGGTCCCCTTCGTCCCTCCAAGATACATGGCTCCTACCGAAGGACGGAACTCTATTAGGTACTCCGAGCT
  CGCGCCACTTTACGATACCACGCGGGTGTATCTGGTCGATAACAAGTCCGCCGATATCGCATCCCTGAAT
  TACCAGAATGATCACAGCAATTTTCTGACAACCGTGGTTCAAAACAACGACTTTACCCCAGCAGAGGCGT
  CAACCCAAACAATTAATTTCGACGAACGCTCCAGATGGGGGGGTGATCTGAAAACCATCCTGCACACAAA
  TATGCCTAATGTGAATGAGTATATGTTCACGAGCAAGTTCAAGGCTCGCGTGATGGTCGCCAGAAAGCAT
  CCACAGGGGGTGGAGGCCACCGATCTTTCTAAGGATATTCTTGAATACGAATGGTTTGAGTTCACATTGC
  CTGAGGGCAACTTTAGCGAGACTATGACAATAGACCTCATGAACAACGCCATCCTTGAGAACTATCTGCA
  GGTGGGTAGGCAGAATGGAGTACTTGAGAGTGACATCGGCGTTAAATTCGATAGCCGGAACTTCAAATTG
  GGCTGGGACCCTGTTACTAAACTTGTCATGCCGGGGGTGTACACGTATGAAGCTTTCCATCCCGATGTCG
  TGCTTTTGCCCGGATGTGGTGTAGACTTTACAGAGTCCAGATTGTCAAACCTCCTTGGAATTCGCAAGAA
  ACAGCCCTTCCAGGAGGGATTTCGGATCATGTACGAGGATCTTGAGGGCGGTAATATCCCCGCACTTCTG
  GATGTCCCTAAGTACCTGGAGTCCAAAAAGAAATTGGAGGAAGCGCTCGAAAATGCTGCCAAAGCAAATG
  GCCCGGCTCGCGGCGATAGTTCTGTGAGTCGCGAAGTCGAAAAAGCCGCCGAGAAAGAGCTCGTGATCGA
  GCCCATTAAACAGGACGACACAAAACGCTCCTATAACCTTATTGAGGGCACCATGGATACACTGTATAGG
  TCCTGGTATTTGTCATACACTTACCGCGACCCAGAGAACGGCGTGCAGAGCTGGACCCTCCTCACTACTC
  CAGATGTTACGTGCGGTGCCGAACAGGTGTATTGGAGTCTTCCTGATCTGATGCAGGATCCCGTCACATT
  CAGATCTACACAACAGGTGTCCAATTACCCAGTCGTCGGAGCAGAGCTCATGCCTTTTCGCGCCAAGAGC
  TTTTACAACGACCTCGCTGTGTACAGCCAGCTGATTCGGTCCTATACATCACTGACGCACGTCTTCAATA
  GGTTTCCAGATAATCAGATCCTCTGCAGACCCCCCGCTCCAACAATAACAACCGTTTCAGAGAACGTTCC
  AGCACTGACGGACCATGGTACACTGCCCCTCCGGTCCTCTATCAGGGGAGTGCAGCGCGTGACCGTCACA
  GATGCCCGCCGGCGCACCTGCCCTTACGTGTACAAGGCACTCGGCATCGTCGCTCCCAGAGTCCTCAGTT
  CACGCACGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100410 representing AP_000147
  Red=Cloning sites Green=Tags
  
  MRRAVVSSSPPPSYESVMAQATLEVPFVPPRYMAPTEGRNSIRYSELAPLYDTTRVYLVDNKSADIASLN
  YQNDHSNFLTTVVQNNDFTPAEASTQTINFDERSRWGGDLKTILHTNMPNVNEYMFTSKFKARVMVARKH
  PQGVEATDLSKDILEYEWFEFTLPEGNFSETMTIDLMNNAILENYLQVGRQNGVLESDIGVKFDSRNFKL
  GWDPVTKLVMPGVYTYEAFHPDVVLLPGCGVDFTESRLSNLLGIRKKQPFQEGFRIMYEDLEGGNIPALL
  DVPKYLESKKKLEEALENAAKANGPARGDSSVSREVEKAAEKELVIEPIKQDDTKRSYNLIEGTMDTLYR
  SWYLSYTYRDPENGVQSWTLLTTPDVTCGAEQVYWSLPDLMQDPVTFRSTQQVSNYPVVGAELMPFRAKS
  FYNDLAVYSQLIRSYTSLTHVFNRFPDNQILCRPPAPTITTVSENVPALTDHGTLPLRSSIRGVQRVTVT
  DARRRTCPYVYKALGIVAPRVLSSRTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000006
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000006.1, AP_000147
• RefSeq ORF: 1551
• MW: 58.5 kDa