E3 gp19K (AC_000006) Virus Tagged ORF Clone

CAT#: VC100415

Myc-DDK-tagged ORF clone of viral ORF for E3 gp19K [Human adenovirus D], codon optimized for human cell expression, AP_000152

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 gp19K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100415 represents NCBI reference of AP_000152 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGCCTGCTTTTGATTATTCTCAGTCTGGTAGGTGGCGTGCTTTCCTGTCACGAACAGCCACGCT
  GCAATATCACCACTGGCAATGAAAGATCAGTGATTTGCACCGTTGTAATTAAGTGTGAGCATACATGCCC
  GCTGAACATTACTTTTAAAAATAGAACAATGGGTAATGCATGGGTTGGGGACTGGGAACCCGGAGATGAA
  CAGAATTACACGGTGACAGTGCACGGGAGTAATGGCAACCATACCTTTGGTTTTAAGTTTATTTTCGAGG
  TCATGTGCGACATTACGCTCCACGTCGCAAGACTTCACGGACTCTGGCCCCCCACCAAGGACAACATGGT
  TGGATTCTCCCTGGCATTCGTCATTATGGCATGCGCAATGAGTGGATTGCTTGTCGGAGCTCTCGTGTGG
  TTCCTGAAGCGAAAGCCCCGATATGGCAATGAGGAGAAGGAGAAGCTGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100415 representing AP_000152
  Red=Cloning sites Green=Tags
  
  MKGLLLIILSLVGGVLSCHEQPRCNITTGNERSVICTVVIKCEHTCPLNITFKNRTMGNAWVGDWEPGDE
  QNYTVTVHGSNGNHTFGFKFIFEVMCDITLHVARLHGLWPPTKDNMVGFSLAFVIMACAMSGLLVGALVW
  FLKRKPRYGNEEKEKLL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000006
• ORF Size: 471 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000006.1, AP_000152
• RefSeq ORF: 471
• MW: 17.5 kDa