pIIIa (AC_000006) Virus Tagged ORF Clone

CAT#: VC100409

Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus D], codon optimized for human cell expression, AP_000146

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pIIIa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100409 represents NCBI reference of AP_000146 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCAGGCTCCTGATCCGGCCATCAGGGCAGCGCTCCAGAGCCAGCCTTCTGGACTGGCATCCGATG
  ACTGGGAGGCAGCGATGCAACGGATTATGGCATTGACCACTCGAAATCCTGAGAGCTTCAGGCAGCAGCC
  TCAGGCCAACCGGTTGAGCGCCATTTTGGAGGCCGTCGTGCCAAGTAGGACCAACCCAACCCACGAGAAA
  GTACTGGCGATCGTAAATGCCCTGGCGGAAAATAAGGCAATACGCCCCGACGAAGCGGGGCTTGTCTACA
  ACGCTCTGCTCGAGAGGGTGGGCAGGTATAATTCCACTAATGTGCAGTCCAACTTGGATAGATTGGTGAC
  CGATGTGCGGGAGGCCGTGGCCCAGAGGGAACGGTTCAAAAATGAGGGCCTGGGAAGCCTTGTGGCATTG
  AACGCCTTTCTCGCTACTCAGCCTGCCAACGTGCCCCGGGGGCAAGATGACTATACAAACTTCATCAGTG
  CCCTGAGACTGATGGTCACCGAGGTCCCACAGTCAGAAGTTTACCAGTCCGGTCCAGACTACTTTTTCCA
  GACAAGCCGGCAGGGGCTTCAGACAGTTAACCTGAGCCAGGCATTTAAAAACCTGAGGGGTCTCTGGGGC
  GTGCAAGCTCCCGTCGGGGACAGATCCACCGTTTCATCTCTCCTGACCCCCAATTCACGACTGTTGCTGC
  TGCTCATAGCTCCATTCACTGATTCCGGTTCAGTGAACAGGAACAGCTACCTGGGACATCTGCTGACTTT
  GTACAGAGAGGCTATTGGACAGGCCCAAGTAGATGAGCAAACTTTCCAGGAAATCACAAGCGTGAGTAGA
  GCCCTGGGTCAGAACGACACGGACAGTCTCCGAGCCACCCTGAACTTTCTGCTCACCAACCGGCAGCAGA
  AGATTCCCGCCCAGTATGCATTGAGTGCCGAGGAGGAAAGGATACTTAGGTATGTTCAGCAAAGCGTGGG
  GCTTTTTCTCATGCAAGAAGGGGCAACCCCCTCTGCAGCCCTCGATATGACAGCTCGAAATATGGAACCT
  AGTATGTATGCTGCGAACCGCCCATTTATCAACAAGCTGATGGACTACCTTCATAGAGCCGCCTCTATGA
  ATTCAGATTACTTTACCAACGCCATTCTCAACCCCCACTGGCTTCCTCCACCAGGCTTTTACACAGGTGA
  GTACGACATGCCTGATCCAAACGATGGATTTCTGTGGGATGATGTCGACTCCGCTGTTTTCTCTCCAACT
  CTCCAGAAACGGCAGGAAGCGGTCCGGACTCCAGCCAGTGAGGGCGCAGTGGGACGGTCCCCATTCCCCT
  CCTTGGGAAGCCTGCACTCCCTGCCTGGCAGTGTGAATAGCGGGAGGGTCAGCAGACCCCGCCTTCTGGG
  AGAAGATGAATATCTCAATGATTCTCTCCTCCAACCTCCAAGAGTAAAGAATGCGATGGCCAACAACGGC
  ATAGAATCACTCGTTGACAAGCTGAATCGGTGGAAGACCTACGCCCAGGACCATAGAGAGCCCGCTCCTG
  CTCCGCGGCGCCAGCGGCACGATAGGCAACGCGGACTCGTATGGGACGATGAAGACTCTGCCGATGACTC
  TAGCGTGCTCGACCTGGGCGGGTCTGGCGGCGTAAATCCCATCTCAAGGATACTGCAGCCCAAGTTGGGC
  CGCCGGATGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100409 representing AP_000146
  Red=Cloning sites Green=Tags
  
  MQQAPDPAIRAALQSQPSGLASDDWEAAMQRIMALTTRNPESFRQQPQANRLSAILEAVVPSRTNPTHEK
  VLAIVNALAENKAIRPDEAGLVYNALLERVGRYNSTNVQSNLDRLVTDVREAVAQRERFKNEGLGSLVAL
  NAFLATQPANVPRGQDDYTNFISALRLMVTEVPQSEVYQSGPDYFFQTSRQGLQTVNLSQAFKNLRGLWG
  VQAPVGDRSTVSSLLTPNSRLLLLLIAPFTDSGSVNRNSYLGHLLTLYREAIGQAQVDEQTFQEITSVSR
  ALGQNDTDSLRATLNFLLTNRQQKIPAQYALSAEEERILRYVQQSVGLFLMQEGATPSAALDMTARNMEP
  SMYAANRPFINKLMDYLHRAASMNSDYFTNAILNPHWLPPPGFYTGEYDMPDPNDGFLWDDVDSAVFSPT
  LQKRQEAVRTPASEGAVGRSPFPSLGSLHSLPGSVNSGRVSRPRLLGEDEYLNDSLLQPPRVKNAMANNG
  IESLVDKLNRWKTYAQDHREPAPAPRRQRHDRQRGLVWDDEDSADDSSVLDLGGSGGVNPISRILQPKLG
  RRMF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000006
• ORF Size: 1692 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000006.1, AP_000146
• RefSeq ORF: 1692
• MW: 62.6 kDa