E3 RID-beta (AC_000006) Virus Tagged ORF Clone

CAT#: VC100417

Myc-DDK-tagged ORF clone of viral ORF for E3 RID-beta [Human adenovirus D], codon optimized for human cell expression, AP_000154

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 RID-beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100417 represents NCBI reference of AP_000154 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATGCAAACTCTGCTCATCCTCCTGTCCCTTCTTAGCCATGCCCTCGCTACCGCTGATTACTCCA
  AGTGCAAGCTGGCGGACATTTGGAATTTCCTGGATTGCTACCAGGAAAAGATCGACATGCCCAGTTACTA
  TCTGGTGATTGTAGGAATTGTTATGGTATGCTCTTGCACATTTTTCGCAATTATGATATATCCCTGCTTT
  GATCTCGGTTGGAACAGTGTGGAGGCTTTTACATACACCCTTGAGAGCTCAAGTCTGGCCTCTACTCCAC
  CTCCCACTCCCCCGCCTAGACGGAACCAGTTTCCAATGATTCAGTACCTTGAAGAGCCGCCCCCAAGACC
  TCCATCTACAGTCTCTTATTTTCACATTACGGGTGGAGACGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100417 representing AP_000154
  Red=Cloning sites Green=Tags
  
  MTMQTLLILLSLLSHALATADYSKCKLADIWNFLDCYQEKIDMPSYYLVIVGIVMVCSCTFFAIMIYPCF
  DLGWNSVEAFTYTLESSSLASTPPPTPPPRRNQFPMIQYLEEPPPRPPSTVSYFHITGGDD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000006
• ORF Size: 393 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000006.1, AP_000154
• RefSeq ORF: 393
• MW: 14.8 kDa