E4 34K (AC_000006) Virus Tagged ORF Clone

CAT#: VC100421

Myc-DDK-tagged ORF clone of viral ORF for E4 34K [Human adenovirus D], codon optimized for human cell expression, AP_000158

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4 34K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100421 represents NCBI reference of AP_000158 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGACCGAAATACAGTCTAGCTCACTTCGCCATCATCCCTACCGCAGGGCTAGATTGCCCCGGAGCG
  ATGACGAGACACGGGCCAGCCTGACTGACCAACATCCTCTTCTGCCAGACTGCGATCACGCCGATTACCA
  CAACGTGAGTTCAGTGAGAGGCCTGCCCTGTGCGGCTGGATTCACCCTCCTGCAAGAGTTTCCTGTGCCG
  TGGGACATGATCCTGACGACTGAAGAAATTAAGATACTGAAAAGGTGCATGAGCGTATGTCTGTGTCCAG
  CCACATTGGATCTGGTCCGGGCGCAGATGGTCTCAGGTTATGAGCGGTGGATCCTGCACTGTCACTGCAG
  CAGCCCCGGGAGTCTGCAGTGCCGGGCCGGTGGCACCCTGTTGGCAGTGTGGTTCCGCAGAGTAATATAC
  GGCTGCATGTTCAACCAGAGATTCCCGTGGTACCGGCAGATAGTAAACCGCAACATGCCCAAGGAAATAA
  TGTACATGGGATCAGTGTTTATGAGAGGTAGGCACCTTATATATTGCAGAATCTGGTATGACGGCCATGT
  CGGCTCCATTATCCCTAATATGTCTTTCGGATGGAGTACCCTGAATTATGGGCTGTTGAACAACATGGTG
  ATAATGTGCTGTACCTACTGCGAGAATATGGCTGAAATCCGCATGCGCTGCTGTGCACGAAGAACCCGCC
  GGCTCATGTTGAAAGCAGTGGGTATTATTGTGCGGGAGACCTGCGACCCTGATCCAATCTGCAGCAGCCG
  AACCGAACCAAGGAGACAACGACTCCTCAGAGCACTGATGGAGCGGCACAGACCCATTCTGTTCTCTGAG
  TATGAGAGCGTACGCTCCTCCCGCTCTACTCGACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100421 representing AP_000158
  Red=Cloning sites Green=Tags
  
  MQTEIQSSSLRHHPYRRARLPRSDDETRASLTDQHPLLPDCDHADYHNVSSVRGLPCAAGFTLLQEFPVP
  WDMILTTEEIKILKRCMSVCLCPATLDLVRAQMVSGYERWILHCHCSSPGSLQCRAGGTLLAVWFRRVIY
  GCMFNQRFPWYRQIVNRNMPKEIMYMGSVFMRGRHLIYCRIWYDGHVGSIIPNMSFGWSTLNYGLLNNMV
  IMCCTYCENMAEIRMRCCARRTRRLMLKAVGIIVRETCDPDPICSSRTEPRRQRLLRALMERHRPILFSE
  YESVRSSRSTRL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000006
• ORF Size: 876 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000006.1, AP_000158
• RefSeq ORF: 876
• MW: 34.1 kDa