pTP (AC_000006) Virus Tagged ORF Clone

CAT#: VC100407

Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus D], codon optimized for human cell expression, AP_000144

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pTP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100407 represents NCBI reference of AP_000144 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGTCCGTGAACGACTGCGCCAGACTGACTGGCCAAACCGTACCTACGATGGATTACTTTCTTC
  CACTGCGCAATATCTGGAATCGCGTGCGAGAGTTCCCTCGGGCGTCAACAACGGCCGCTGGTATCACATG
  GATGTCCAGGTACCTGTACGGGTACCATCGGTTGATGCTGGAAGATCTGGCCCCTGGAGCTCCCGCCACT
  CAGCGGTGGCCCCTGTACCGCCAGCCTCCCCCACACTTCCTGGTCGGCTACCAGTACCTGGTGAGGACTT
  GTAATGATTATGTCTTCGATAGCAGAGCCTTTTCTCGGCTCAGATATAGCGAGGTGGTGCAGCCCGGATT
  GCAGACAGTTAATTGGTCCCTGATGGCCAATTGTACCTACACCATTAATACGGGAGCATACCACAGATTC
  GTCGATATGGATGACTTTCAAGATACTCTGACTAGGGTTCAGCAGGCTATTCTCGCCGAAAGAGTAGTTG
  CTGACCTGGCTCTGGTCCAGCCTCTCAGGGGGGTGGGCGTGACAAGGATGGAGGACAGCGCAAGTGCATC
  CGATGATATCGAACGGCTCATGCACGATTATTATAAAAACCTGTCTCGATGCCAGGGACAGGCTTGGGGT
  ATGGCCGAACGCCTCCGCATACAACAGGCTGGTCCAAAGGATCTTGTCCTTCTGGCCACTATTAGGCGAC
  TGAAGAACGCATACTTTAATTATATCATCTCTAATAGGAACTCAAACTCTGTCCATCGGGCAGCCACCTG
  TCTGTCCCTTCCATGTGATTGTGACTGGCTGGATGCGTTCTTGGAGCGCTTTTCAGACCCAGTGGACCTG
  GATGCTCTGACCTCCCCAACCCCTCAGCTGATCCGCTGCATAGTGTCCGCGCTCTCACTGCCTAACGGGG
  ATCCTCCCCACTACCGGGAGATGACCGGAGGAGTGTTCACACTCAGACCGCGCGAACGAGGCAGAGCTGT
  TACCGAAACGATGAGGAGGCGACGCGGGGAAATGATTGAGAGATTCGTGGATCGCTTGCCCGTCCGAAGG
  AGGCGCAGACGCGCACCCCCTCCCCCCCCGCCTCCGGAGGAGGAGATCGAGGAAGTTGTTATGGAAGAGG
  AAGAAGAGGATGAAGCTCCCGGCGACTTTGAGAGGGAGGTTCGCGCTACAATCGCAGAACTGATCCGACT
  CCTGGAGGATGAGCTTACTGTGTCCGCACGCAACGCTCAGTTTTTCAATTTTGCTGTGGATTTCTACGAA
  GCAATGGAGCGGCTGGAAGCTATCGGAGACATCTCAGAGATGCCTCTCAGAAGGTGGATTATGTATTTTT
  TCGTGACGGAGCACATAGCAACAACACTCAATTACTTGTTTCAACGGCTGCGGAATTACGCTGTGTTCGC
  AAGGCACGTTGAGCTCAACCTGGCACAGGTGGTCATGCGCGCTCGGGACGCTGACGGTGACGTTGTATAC
  AGTCGGGTTTGGAACGAGAGCGGTCTCGGGGCTTTTTCTCAACTGATGGGACGCATAAGTAATGACCTCG
  CCGCAACCGTAGAGCGAGCAGGGAGGGGCGACTTGCAGGAGGAAGAGATTGAACAGTTTATGTCAGAGAT
  CGCCTATCAGGATAACTCTGGGGACGTGCAAGAAATTCTCAGGCAGCCAGCCGTCAATGACGCAGAGATC
  GATAGCGTGGAGCTGAGTTTCAGATTCAAGGTGACCGGTCCAGTTGTGTTCACCCAGAGAAGACAGATTC
  AGGACGTCAACCGGCGCGTGGTCGCCCACGCCTCTGCACTTCGCGCGCAACATAGAGACCTGCCAGAGAG
  GCATGCAGATGTTCCCCTTCCACCCTTGCCTGCAGGGCCGGAGCCACCACTGCCCCCTGGAGCAAGGCCC
  CGACATAGATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100407 representing AP_000144
  Red=Cloning sites Green=Tags
  
  MALSVNDCARLTGQTVPTMDYFLPLRNIWNRVREFPRASTTAAGITWMSRYLYGYHRLMLEDLAPGAPAT
  QRWPLYRQPPPHFLVGYQYLVRTCNDYVFDSRAFSRLRYSEVVQPGLQTVNWSLMANCTYTINTGAYHRF
  VDMDDFQDTLTRVQQAILAERVVADLALVQPLRGVGVTRMEDSASASDDIERLMHDYYKNLSRCQGQAWG
  MAERLRIQQAGPKDLVLLATIRRLKNAYFNYIISNRNSNSVHRAATCLSLPCDCDWLDAFLERFSDPVDL
  DALTSPTPQLIRCIVSALSLPNGDPPHYREMTGGVFTLRPRERGRAVTETMRRRRGEMIERFVDRLPVRR
  RRRRAPPPPPPPEEEIEEVVMEEEEEDEAPGDFEREVRATIAELIRLLEDELTVSARNAQFFNFAVDFYE
  AMERLEAIGDISEMPLRRWIMYFFVTEHIATTLNYLFQRLRNYAVFARHVELNLAQVVMRARDADGDVVY
  SRVWNESGLGAFSQLMGRISNDLAATVERAGRGDLQEEEIEQFMSEIAYQDNSGDVQEILRQPAVNDAEI
  DSVELSFRFKVTGPVVFTQRRQIQDVNRRVVAHASALRAQHRDLPERHADVPLPPLPAGPEPPLPPGARP
  RHRF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000006
• ORF Size: 1902 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000006.1, AP_000144
• RefSeq ORF: 1902
• MW: 72.8 kDa