52K (AC_000006) Virus Tagged ORF Clone

CAT#: VC100408

Myc-DDK-tagged ORF clone of viral ORF for 52K [Human adenovirus D], codon optimized for human cell expression, AP_000145

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 52K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100408 represents NCBI reference of AP_000145 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCCCGTTCTGCGGCAGATGCGACCTACCCCCCCCGCAACCACAGCGACCGCAGCAGTAGCTGGCG
  CCGGGGCCAGCCAACCACAGCCACAGACCGAAATGGATCTTGAGGAGGGAGAGGGTTTGGCCCGACTGGG
  TGCTCCCAGCCCGGAACGACACCCAAGGGTGCAGCTGCAGAAAGACGTCAGACCAGCATATGTCCCCGCC
  CAGAACCTGTTCCGAGATCGGTCTGGCGAAGAGCCAGAAGAGATGAGAGATTGCCGATTTCGAGCTGGCC
  GCGAGCTCCGGGAAGGCCTGGACAGACAAAGGGTCCTGAGAGATGAGGACTTTGAGCCTAATGAGCAAAC
  CGGGATCAGCCCAGCCAGAGCACACGTGGCCGCCGCAAACCTGGTGACTGCCTACGAGCAAACCGTTAAA
  CAGGAGAGAAACTTCCAAAAAAGCTTTAACAACCACGTCAGGACACTTATTGCCCGCGAAGAGGTCGCCC
  TGGGGCTTATGCACCTCTGGGATCTGGCTGAGGCCATAGTCCAGAATCCAGACAGCAAACCTCTCACTGC
  CCAGCTTTTTCTTGTCGTGCAGCATAGTCGGGACAATGAGGCCTTCAGAGAGGCCCTGCTTAATATAGCT
  GAGCCAGAAGGAAGGTGGCTGCTGGAACTGATTAACATTCTTCAGTCCATTGTCGTGCAGGAAAGGTCAT
  TGTCTCTGGCAGAAAAAGTGGCAGCTATCAATTACTCCGTACTCTCCCTTGGGAAGTTTTACGCCAGAAA
  AATTTACAAAACCCCCTATGTCCCCATCGATAAAGAGGTTAAAATCGATTCTTTTTATATGAGAATGGCT
  CTGAAGGTCTTGACCCTTTCCGATGACCTGGGCGTATATAGGAACGACAGGATACACAAGGCAGTCAGCG
  CCAGCCGGCGCAGGGAACTCAGCGATAGGGAACTGATGCTCTCTCTCAGAAGAGCACTGGTTGGAGGTGC
  CGCTGGCGGCGAAGAATCCTACTTCGACATGGGAGCAGACCTCCATTGGCAGCCCAGCCGCCGCGCCTTG
  GAGGCAGCCTATGGACCAGAGGACCTGGATGAAGAGGAAGAGGAGGAAGAGGATGCGCCTGCAGCCGGGT
  AC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100408 representing AP_000145
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPTPPATTATAAVAGAGASQPQPQTEMDLEEGEGLARLGAPSPERHPRVQLQKDVRPAYVPA
  QNLFRDRSGEEPEEMRDCRFRAGRELREGLDRQRVLRDEDFEPNEQTGISPARAHVAAANLVTAYEQTVK
  QERNFQKSFNNHVRTLIAREEVALGLMHLWDLAEAIVQNPDSKPLTAQLFLVVQHSRDNEAFREALLNIA
  EPEGRWLLELINILQSIVVQERSLSLAEKVAAINYSVLSLGKFYARKIYKTPYVPIDKEVKIDSFYMRMA
  LKVLTLSDDLGVYRNDRIHKAVSASRRRELSDRELMLSLRRALVGGAAGGEESYFDMGADLHWQPSRRAL
  EAAYGPEDLDEEEEEEEDAPAAGY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000006
• ORF Size: 1122 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000006.1, AP_000145
• RefSeq ORF: 1122
• MW: 42.2 kDa