fiber (AC_000006) Virus Tagged ORF Clone

CAT#: VC100420

Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus D], codon optimized for human cell expression, AP_000157

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: fiber
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100420 represents NCBI reference of AP_000157 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAAACGACTCAGAGTCGAGGACGATTTTAACCCCGTTTACCCTTATGGATATGCCAGGAACCAGA
  ATATACCCTTTCTCACTCCCCCCTTTGTCTCATCTGATGGGTTTAAGAATTTCCCCCCTGGCGTCCTTTC
  CCTGAAACTGGCGGATCCTATCACCATTGCCAACGGAGATGTGTCCCTGAAGGTGGGTGGCGGCCTCACA
  TTGCAGGAAGGCTCTATGACCGTAGATCCAAAAGCACCCCTGCAGCTGGCTAATAACAAAAAGTTGGAAC
  TGGTGTACGTCGATCCATTCGAGGTATCTGCAAATAAGCTCAGTCTTAAGGTGGGACATGGTCTCAAGAT
  CCTGGACGATAAATCCGCAGGAGGGTTGAAGGATCTGATTGGGAAGCTTGTAGTACTGACTGGAAAGGGG
  ATTGGGACAGAAAACTTGCAAAATACGGATGGATCTTCTCGCGGAATCGGTATTTCTGTCCGCGCTCGAG
  AGGGTCTTACCTTTGACAACGACGGCTATCTTGTGGCGTGGAATCCTAAGTATGACACAAGAACCCTGTG
  GACTACCCCTGATACTTCTCCTAATTGCCGAATCGATAAAGAGAAGGACTCTAAGTTGACTCTCGTACTG
  ACTAAGTGTGGGTCCCAGATCCTGGCAAACGTGTCTCTGATTGTTGTAAGTGGCAAGTACCAGTACATTG
  ACCACGCCACAAACCCGACTTTGAAGTCCTTCAAGATCAAGCTTCTCTTCGATAACAAGGGCGTGCTTCT
  CCCCAGCAGTAACCTGGACTCTACGTACTGGAATTTTCGATCTGACAATCTTACCGTGAGTGAAGCCTAC
  AAGAACGCTGTGGAATTCATGCCCAACTTGGTGGCCTACCCCAAGCCGACTACCGGCTCTAAAAAGTACG
  CGCGCGACATTGTGTATGGAAATATCTACCTGGGAGGCCTGGCCTATCAGCCGGTGGTGATTAAAGTGAC
  GTTCAATGAAGAAGCGGATAGCGCCTACAGTATCACATTCGAGTTCGTCTGGAACAAGGAGTACGCCAGA
  GTTGAGTTCGAAACAACCTCTTTCACTTTTAGTTACATTGCCCAGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100420 representing AP_000157
  Red=Cloning sites Green=Tags
  
  MSKRLRVEDDFNPVYPYGYARNQNIPFLTPPFVSSDGFKNFPPGVLSLKLADPITIANGDVSLKVGGGLT
  LQEGSMTVDPKAPLQLANNKKLELVYVDPFEVSANKLSLKVGHGLKILDDKSAGGLKDLIGKLVVLTGKG
  IGTENLQNTDGSSRGIGISVRAREGLTFDNDGYLVAWNPKYDTRTLWTTPDTSPNCRIDKEKDSKLTLVL
  TKCGSQILANVSLIVVSGKYQYIDHATNPTLKSFKIKLLFDNKGVLLPSSNLDSTYWNFRSDNLTVSEAY
  KNAVEFMPNLVAYPKPTTGSKKYARDIVYGNIYLGGLAYQPVVIKVTFNEEADSAYSITFEFVWNKEYAR
  VEFETTSFTFSYIAQQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000006
• ORF Size: 1098 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000006.1, AP_000157
• RefSeq ORF: 1098
• MW: 40.5 kDa