beta III Tubulin (TUBB3) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse, Rat |
| Immunogen | Synthetic peptide corresponding to a region of the Tuj1 gene product shared between the Rat (AAM28438, NCBI) and Human (AAL28094, NCBI) gene products. |
beta III Tubulin (TUBB3) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Mouse |
| Immunogen | TUBB3 / Tubulin Beta 3 antibody was raised against three synthetic peptides corresponding to different regions of the beta-Tubulin 3 (KLH). |
GFAP chicken polyclonal antibody, Serum
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | GFAP antibody was raised against full-length recombinant mouse GFAP |
GFP chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Immunogen | Purified recombinant Green Fluorescent Protein (GFP) emulsified in Freund's adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Purified antibody preparations were then mixed with Glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with Thimerosal, and then filter-sterilized. |
CNPase (CNP) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | Synthetic peptide KLH conjugated corresponded to a region of the CNPase gene product shared between the Human (NP_149124) and Mouse (P16330) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks. |
NSE (ENO2) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse, Rat |
| Immunogen | Chickens were immunized with two synthetic peptide KLH conjugates corresponding to different regions of the NSE-2 gene product, but are shared between the Human (NP_001966, NCBI) and Rat (AAA41119) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of each of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Peripherin chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Rat |
| Immunogen | Full-length recombinant Peripherin protein. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. |
Synaptotagmin 1 (SYT1) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | Four different antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (NP_033332) and Human (NP_005630) gene products. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.4 mg/ml. Finally, equal volumes of each of the four affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. Antibodies were affinity-purified and mixed in equal concentrations. |
beta III Tubulin (TUBB3) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Rat |
| Immunogen | Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Beta-Tubulin 3 gene product, but are shared between the Human (AAL28094) and Rat (AAM28438) protein sequences. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Tyrosine Hydroxylase (TH) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | Two antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (P24529) and Human (P07101) gene products. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of both of these affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Vimentin (VIM) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Vimentin gene product, but are shared between the Human (NP_003371) and Mouse (NP_035831) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Doublecortin (DCX) chicken polyclonal antibody, Purified
| Applications | IF, IHC |
| Reactivities | Human, Mouse |
| Immunogen | Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the Doublecortin gene product, but are shared between the Human (CAA06617.1, NCBI) and Mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
mGluR1a / GRM1a chicken polyclonal antibody, Purified
| Applications | IF, IHC |
| Reactivities | Human, Mouse |
| Immunogen | Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 1 gene product, but are shared between the human (NP_000829.2, NCBI) and mouse (NP_058672.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Metabotropic Glutamate Receptor 5 (GRM5) chicken polyclonal antibody, Purified
| Applications | IF, IHC |
| Reactivities | Human, Mouse |
| Immunogen | Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 5 gene product, but are shared between the Human (NP_001137303.1, NCBI) and Mouse (Q3UVX5.2, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
GAP43 (C-term) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Mouse |
| Immunogen | Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of Mouse GAP-43 coupled to keyhole limpet hemocyanin. Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1/10 with PBS containing BSA as a carrier. Finally, the antibody preparation was filter-sterilized. |
RET chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IF, WB |
| Reactivities | Human |
| Immunogen | Recombinant His-tagged extracellular fragment of human RET protein produced using CHO-based. The extracellular fragment of hRET was expressed and secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant. |
GFR alpha 3 (GFRA3) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IF, WB |
| Reactivities | Human |
| Immunogen | Recombinant His-tagged human GFRa-3 protein produced using CHO-based. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant. |
Grm5 chicken polyclonal antibody
| Applications | IF, IHC |
| Reactivities | Human, Mouse |
| Conjugation | Unconjugated |
GAD67 (GAD1) chicken polyclonal antibody
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Immunogen | Synthetic peptide (keyhole limpet hemocyanin -KLH conjugate) corresponding to a region near the C-terminus of this gene product; 100% conserved between human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) |
PIN1 chicken polyclonal antibody, Serum
| Applications | IF, WB |
| Reactivities | Human |
| Immunogen | Recombinant full length Pin-1 purified from E. coli. |
Peripherin (PRPH) chicken polyclonal antibody, Serum
| Applications | IF, WB |
| Reactivities | Canine, Human, Mouse, Rat |
| Immunogen | Rat peripherin was expressed in bacteria and purified from inclusion bodies. |
Coronin 1a (CORO1A) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC |
| Reactivities | Human, Mouse |
| Immunogen | Synthetic peptide KLH conjugated corresponding to a sequence shared between the Mouse (NP_034028) and Human (NP_009005) gene products. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks. |
Netrin 1 (NTN1) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC |
| Reactivities | Human, Mouse |
| Immunogen | Synthetic peptide KLH conjugated corresponding to the netrin-1 gene product, but was shared between the Human (O95631) and Mouse (O9118) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 0.1 mg/ml, and the preparation filter-sterilized. |
Acpp (Pain System Marker) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC |
| Reactivities | Mouse |
| Immunogen | Recombinant Mouse PAP protein was expressed using a baculoviral-delivery system. Preparation: After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared (“anti-PAP IgY fraction”). Some of this antibody was further purified using an agarose matrix to which the PAP protein was convalently attached (“Affinity-purified anti-PAP”). The final preparation in the accompanying vial contains 10 mg/ml of the “anti-PAP IgY fraction” supplemented with 20 mg/ml of the “affinity-purified anti-PAP” plus 50% (v/v) Glycerol (to prevent freezing at –20°C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 µl aliquots prepared. |
PRNP chicken polyclonal antibody, Purified
| Applications | IF, WB |
| Reactivities | Human, Mouse, Rat |
| Immunogen | Chickens were immunized with a recombinant fragment of the human prion protein emulsified with Freund’s complete and incomplete adjuvants. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Affinity-purified antibodies were then prepared using an agarose column to which the recombinant protein fragments were covalently attached. Finally, affinity purified antibodies (50 ug/ml final concentration) were mixed with the IgY fraction (10 mg/ml final concentration) in a mixture containing glycerol (50% v/v) (to prevent freezing at -20°C) and 0.02% Sodium Azide. |
Amyloid beta A4 protein / APP (501-522) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC |
| Reactivities | Human, Mouse, Rat |
| Immunogen | Chickens were immunized with synthetic peptide APP#4 conjugated to keyhole limpet hemocyanin (KLH). The sequence of APP peptide #4 is EQK DRQ HTL KHF EHV RMV DPK K, which corresponds to residues #501-522 of the Human gene product (NP_000475.1)]. Identical sequences are also found in the Mouse and Rat homologs of APP. |
Amyloid beta A4 protein / APP (556-575) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC |
| Reactivities | Human, Mouse, Rat |
| Immunogen | Chickens were immunized with synthetic peptide APP#5 conjugated to keyhole limpet hemocyanin (KLH). The sequence of APP peptide #5 is EEI QDE VDE LLQ KEQ NYS DD, which corresponds to residues #556-575 of the Human gene product (NP_000475.1)]. Identical sequences are also found in the Mouse and Rat homologs of APP. |
Germany
Japan
United Kingdom
China
