Primary Antibodies

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Peripherin chicken polyclonal antibody, Aff - Purified

Applications IF, IHC, WB
Reactivities Rat
Immunogen Full-length recombinant Peripherin protein.
Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks.

Synaptotagmin 1 (SYT1) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC, WB
Reactivities Human, Mouse
Immunogen Four different antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (NP_033332) and Human (NP_005630) gene products.
Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.4 mg/ml. Finally, equal volumes of each of the four affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Antibodies were affinity-purified and mixed in equal concentrations.

beta III Tubulin (TUBB3) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC, WB
Reactivities Human, Rat
Immunogen Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Beta-Tubulin 3 gene product, but are shared between the Human (AAL28094) and Rat (AAM28438) protein sequences.
Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Tyrosine Hydroxylase (TH) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC, WB
Reactivities Human, Mouse
Immunogen Two antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (P24529) and Human (P07101) gene products.
Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of both of these affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Vimentin (VIM) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC, WB
Reactivities Human, Mouse
Immunogen Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Vimentin gene product, but are shared between the Human (NP_003371) and Mouse (NP_035831) sequences.
Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Doublecortin (DCX) chicken polyclonal antibody, Purified

Applications IF, IHC
Reactivities Human, Mouse
Immunogen Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates.
Production: These synthetic peptides corresponded to different regions of the Doublecortin gene product, but are shared between the Human (CAA06617.1, NCBI) and Mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

mGluR1a / GRM1a chicken polyclonal antibody, Purified

Applications IF, IHC
Reactivities Human, Mouse
Immunogen Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 1 gene product, but are shared between the human (NP_000829.2, NCBI) and mouse (NP_058672.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Metabotropic Glutamate Receptor 5 (GRM5) chicken polyclonal antibody, Purified

Applications IF, IHC
Reactivities Human, Mouse
Immunogen Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 5 gene product, but are shared between the Human (NP_001137303.1, NCBI) and Mouse (Q3UVX5.2, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

GAP43 (C-term) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC, WB
Reactivities Mouse
Immunogen Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of Mouse GAP-43 coupled to keyhole limpet hemocyanin. Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1/10 with PBS containing BSA as a carrier. Finally, the antibody preparation was filter-sterilized.

Amyloid Precursor Protein (APP) chicken polyclonal antibody, Purified

Applications ELISA, IHC, WB
Reactivities Human, Mouse, Rat
Immunogen Synthetic peptide derived from Human Amyloid beta A4 protein precursor

Kir2.1 (KCNJ2) chicken polyclonal antibody, Purified

Applications ELISA, IHC, WB
Reactivities Human
Immunogen Synthetic peptide derived from C-term domain of human Kir2.1

GFAP chicken polyclonal antibody

Applications IF, IHC, WB
Reactivities Feline, Human, Mouse, Primate, Rat
Conjugation Unconjugated

Choline Acetyltransferase (CHAT) chicken polyclonal antibody

Applications IF, IHC, WB
Reactivities Human, Mouse
Conjugation Unconjugated

Netrin 1 (NTN1) chicken polyclonal antibody

Applications IF, IHC, WB
Reactivities Human, Mouse
Conjugation Unconjugated

NSE (ENO2) chicken polyclonal antibody

Applications IF, IHC, WB
Reactivities Human, Mouse
Conjugation Unconjugated

Vimentin (VIM) chicken polyclonal antibody

Applications IF, IHC, WB
Reactivities Human, Mouse
Conjugation Unconjugated

GFAP chicken polyclonal antibody

Applications IF, IHC, WB
Reactivities Human, Mouse
Conjugation Unconjugated

CNPase (CNP) chicken polyclonal antibody

Applications IF, IHC
Reactivities Human, Mouse
Conjugation Unconjugated

NeuN (RBFOX3) chicken polyclonal antibody

Applications IF, IHC
Reactivities Mouse, Rat
Conjugation Unconjugated

Grm5 chicken polyclonal antibody

Applications IF, IHC
Reactivities Human, Mouse
Conjugation Unconjugated

GAD67 (GAD1) chicken polyclonal antibody

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen Synthetic peptide (keyhole limpet hemocyanin -KLH conjugate) corresponding to a region near the C-terminus of this gene product; 100% conserved between human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI)

Chicken Polyclonal erbB-2 Antibody

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen erbB-2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human erbB-2.

Chicken Polyclonal Transthyretin Antibody

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen Transthyretin antibody was raised against a 17 amino acid peptide near the center of human Transthyretin .

Chicken Polyclonal AP2M1 Antibody

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen AP2M1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human AP2M1.

Chicken Polyclonal ADAMTSL5 Antibody

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen ADAMTSL5 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ADAMTSL5.

Chicken Polyclonal CCDC69 Antibody

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen CCDC69 antibody was raised against a 16 amino acid peptide near the amino terminus of human CCDC69.

Chicken Polyclonal ENC-1 Antibody

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen ENC-1 antibody was raised against a 13 amino acid peptide near the center of human ENC-1.

Chicken Polyclonal ECRG2 Antibody

Applications IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen ECRG2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human ECRG2.

Myostatin Propeptide (MSTN) chicken polyclonal antibody, Purified

Applications ELISA, IHC
Reactivities Human
Immunogen Recombinant Human Myostatin.

Myostatin Propeptide (MSTN) chicken polyclonal antibody, Purified

Applications ELISA, IHC
Reactivities Human
Immunogen Recombinant Human Myostatin.

DNMT1 (177-550) chicken polyclonal antibody, Azide Free

Applications IHC, WB
Reactivities Human
Immunogen Recombinant human DNMT1 (aa 177-550)

DUT (1-52) chicken polyclonal antibody, Azide Free

Applications IHC, WB
Reactivities Human
Immunogen Recombinant Human DUT
Epitope: aa 1-52

IKK beta (IKBKB) (310-376) chicken polyclonal antibody, Azide Free

Applications IHC, WB
Reactivities Human, Mouse, Rat
Immunogen Recombinant Human IKBKB / IKK Beta
aa310-376

Coronin 1a (CORO1A) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC
Reactivities Human, Mouse
Immunogen Synthetic peptide KLH conjugated corresponding to a sequence shared between the Mouse (NP_034028) and Human (NP_009005) gene products.
After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks.

Netrin 1 (NTN1) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC
Reactivities Human, Mouse
Immunogen Synthetic peptide KLH conjugated corresponding to the netrin-1 gene product, but was shared between the Human (O95631) and Mouse (O9118) sequences.
Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 0.1 mg/ml, and the preparation filter-sterilized.

Acpp (Pain System Marker) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC
Reactivities Mouse
Immunogen Recombinant Mouse PAP protein was expressed using a baculoviral-delivery system.
Preparation: After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared (“anti-PAP IgY fraction”). Some of this antibody was further purified using an agarose matrix to which the PAP protein was convalently attached (“Affinity-purified anti-PAP”). The final preparation in the accompanying vial contains 10 mg/ml of the “anti-PAP IgY fraction” supplemented with 20 mg/ml of the “affinity-purified anti-PAP” plus 50% (v/v) Glycerol (to prevent freezing at –20°C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 µl aliquots prepared.

Amyloid beta A4 protein / APP (501-522) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC
Reactivities Human, Mouse, Rat
Immunogen Chickens were immunized with synthetic peptide APP#4 conjugated to keyhole limpet hemocyanin (KLH).  The sequence of APP peptide #4 is EQK DRQ HTL KHF EHV RMV DPK K, which corresponds to residues #501-522 of the Human gene product (NP_000475.1)].  
Identical sequences are also found in the Mouse and Rat homologs of APP.

Amyloid beta A4 protein / APP (556-575) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC
Reactivities Human, Mouse, Rat
Immunogen Chickens were immunized with synthetic peptide APP#5 conjugated to keyhole limpet hemocyanin (KLH).  The sequence of APP peptide #5 is EEI QDE VDE LLQ KEQ NYS DD, which corresponds to residues #556-575 of the Human gene product (NP_000475.1)]. 
Identical sequences are also found in the Mouse and Rat homologs of APP.

ITM2B chicken polyclonal antibody, Aff - Purified

Applications IHC, WB
Reactivities Human
Immunogen Peptide coupled to carrier protein. Amino terminal sequence of BRI protein (ITM2B)

CD88 / C5R1 chicken polyclonal antibody, Aff - Purified

Applications IHC
Reactivities Mouse, Rat
Immunogen Synthetic C-terminal peptide coupled to carrier protein. The antibody was generated against the Cytoplasmic carboxy-terminal portion of the mouse C5aR.

MRP8/14 (S100A8/A9) chicken polyclonal antibody, Aff - Purified

Applications ELISA, IHC
Reactivities Bovine, Human, Porcine
Immunogen Recombinant MRP8 and MRP14

MRP8/14 (S100A8/A9) chicken polyclonal antibody, Biotin

Applications ELISA, IHC
Reactivities Bovine, Human, Porcine
Conjugation Biotin
Immunogen Recombinant Human MRP8 and MRP14. 
Antigen, epitope: MRP8 and MRP14 and their Ca2+-dependent complex. Several epitopes.

Ntsr1 chicken polyclonal antibody

Applications IF, IHC
Reactivities Mouse
Conjugation Unconjugated
Immunogen TVGTHNGLEHSTFNMTIEPGRVQAYC

NFH (NEFH) chicken polyclonal antibody

Applications IHC
Reactivities Human, Mouse
Conjugation Unconjugated

Coronin 1a (CORO1A) chicken polyclonal antibody

Applications IF, IHC
Reactivities Human, Mouse
Conjugation Unconjugated

Metabotropic Glutamate Receptor 2 (GRM2) chicken polyclonal antibody

Applications IF, IHC
Reactivities Human, Mouse
Conjugation Unconjugated

Metabotropic Glutamate Receptor 3 (GRM3) chicken polyclonal antibody

Applications IF, IHC
Reactivities Human, Mouse
Conjugation Unconjugated

MAP2 chicken polyclonal antibody

Applications IF, IHC
Reactivities Human, Mouse
Conjugation Unconjugated
Immunogen Synthetic peptide (keyhole limpet hemocyanin -KLH conjugate) corresponding two different regions of the MAP2 gene, shared between human (NP_002365, NCBI), mouse (P20357, NCBI) sequences

Doublecortin (DCX) chicken polyclonal antibody

Applications IF, IHC
Reactivities Human, Mouse
Conjugation Unconjugated
Immunogen Synthetic peptide (keyhole limpet hemocyanin -KLH conjugate) corresponding two different regions of the doublecortin gene, shared between human (CAA06617.1, NCBI), mouse (AAT58219.1, NCBI) sequences

Chicken Polyclonal TINP1 Antibody

Applications IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen TINP1 antibody was raised against a 13 amino acid peptide near the carboxy terminus of human TINP1.