Secondary Antibodies

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Equine IgM (Fc specific) Goat Polyclonal Antibody
AP21450SU-N

USD 270.00

2 Weeks

Equine IgM (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in horse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgM from serum or plasma.
Directions for use:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal horse serum in agar-block immunodiffusion titration.
Reactivities Equine
Immunogen Highly purified normal IgM isolated from pooled horse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgA (Secretory component) Goat Polyclonal Antibody
AP21476SU-N

USD 270.00

2 Weeks

Human IgA (Secretory component) goat polyclonal antibody, Serum

Applications Can be used in immunoelectrophoresis use 2 µl serum or equivalent against 120 µl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in 3 mm diameter centre well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre 1/64 when tested against pooled normal human milk in agar-block immunodiffusion titration.
Reactivities Human
Immunogen Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM. Purified free human secretory component isolated from pooled milk is used for immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgD (Fc specific) Goat Polyclonal Antibody
AP21479AF-N

USD 410.00

2 Weeks

Human IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications This antiserum is intended to detect and identify IgD in serum or other body fluids using the Immunofixation technique. Like Immunoelectrophoresis Immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.
Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins.
Antibody titre: Precipitin titre not less than 1:16 when tested against an appropriate reference in agar immunodiffusion block titration.
Reactivities Human
Immunogen Highly purified monoclonal IgD isolated from Human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgD (Fc specific) Goat Polyclonal Antibody
AP21479SU-N

USD 240.00

2 Weeks

Human IgD (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgD in human serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of human IgD from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended dilutions:
Immunoelectrophoresis: 2 µl serum or equivalent against 120 µl antiserum.
In double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre 1:32 when tested against pooled an appropriate reference in agar-block immunodiffusion titration.
Reactivities Human
Immunogen Highly purified monoclonal IgD isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgE (Fc specific) Goat Polyclonal Antibody
AP21480PU-N

USD 410.00

2 Weeks

Human IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications This antiserum is intended to detect and identify IgE in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.
Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Antibody titre: Precipitin titre not less than 1/16 when tested against an appropriate reference in agar immunodiffusion block titration.
Reactivities Human
Immunogen Highly purified monoclonal IgE isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgE (Fc specific) Goat Polyclonal Antibody
AP21480SU-N

USD 270.00

2 Weeks

Human IgE (Fc specific) goat polyclonal antibody, Serum

Applications In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgE in human serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of human IgE from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended dilutions:
Immunoelectrophoresis: 2 µl serum or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Antibody titre Precipitin titre 1/32 when tested against pooled an appropriate reference in agar-block immunodiffusion titration.
Reactivities Human
Immunogen Highly purified monoclonal IgE isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgE (Fc specific) Goat Polyclonal Antibody
AP21483SU-N

USD 270.00

2 Weeks

Rat IgE (Fc specific) goat polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgE in Rat serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of Rat IgE from serum or plasma.
This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl serum or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Precipitin Titre: 1/32 when tested against appropriate concentrations of rat IgE in agar-block immunodiffusion titration.
Reactivities Rat
Immunogen Highly purified homogenous IgE isolated from Rat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rabbit IgM (Fc specific) Goat Polyclonal Antibody
AP21488SU-N

USD 270.00

2 Weeks

Rabbit IgM (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in rabbit serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rabbit IgM from serum or plasma.
Recommended dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): A rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration.
Reactivities Rabbit
Immunogen Highly purified normal IgM isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rabbit IgA (Fc specific) Goat Polyclonal Antibody
AP21489SU-N

USD 270.00

2 Weeks

Rabbit IgA (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in rabbit serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rabbit IgA from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended dilutions:
Immunoelectrophoresis: 2 µl serum or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): A rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration.
Reactivities Rabbit
Immunogen Highly purified normal IgA isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rabbit IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP21490SU-N

USD 270.00

2 Weeks

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal rabbit serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended Dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion: a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against normal rabbit serum in agar block titration.
Reactivities Rabbit
Immunogen Purified Normal IgG and fractions containing IgA, and IgM isolated from Rabbit serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Rat IgG2b (subclass specific) Goat Polyclonal Antibody
AP21491SU-N

USD 270.00

2 Weeks

Rat IgG2b (subclass specific) goat polyclonal antibody, Serum

Applications In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2b in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG2b from serum or plasma.
Recommended working dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less then 1/32 when tested against pooled normal rat serum in agar block immunodiffusion titration.
Reactivities Rat
Immunogen Pools of purified homogenous IgG2b isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgG3 (subclass specific) Goat Polyclonal Antibody
AP21494SU-N

USD 270.00

2 Weeks

Mouse IgG3 (subclass specific) goat polyclonal antibody, Serum

Applications Can be used in Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
In double radial immunodiffusion (Ouchterlony): use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not les than 1/32 when tested against pooled normal mouse serum in agar block immunodiffusion titration.
Reactivities Mouse
Immunogen Pools of purified homogenous IgG3 isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (Fc specific) Goat Polyclonal Antibody
AP31436SU-N

USD 270.00

2 Weeks

Monkey IgA + IgG + IgM (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal monkey serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Directions for use:
In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum.
In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Purified normal IgG, IgA and IgM fractions isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA (Fc specific) Goat Polyclonal Antibody
AP31437SU-N

USD 270.00

2 Weeks

Monkey IgA (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in monkey serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgA from serum or plasma. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Directions for use:
In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgA isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (Fc specific) Goat Polyclonal Antibody
AP31438SU-N

USD 270.00

2 Weeks

Monkey IgG (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in monkey serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgG from serum or plasma.
Directions for use:
In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgM (Fc specific) Goat Polyclonal Antibody
AP31440SU-N

USD 270.00

2 Weeks

Monkey IgM (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in monkey serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgM from serum or plasma.
Directions for use:
In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl in a 3 mm diameter center well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgM isolated from pooled Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgA (Fc specific) Goat Polyclonal Antibody
AP31539BT-N

USD 410.00

2 Weeks

Canine IgA (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000.
Reactivities Canine
Conjugation Biotin
Immunogen Purified normal IgA isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgA (Fc specific) Goat Polyclonal Antibody
AP31539SU-N

USD 270.00

2 Weeks

Canine IgA (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in dog serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgA from serum or plasma.
Directions for use:
In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Reactivities Canine
Immunogen Highly purified normal IgA isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgG (Fc specific) Goat Polyclonal Antibody
AP31540BT-N

USD 410.00

2 Weeks

Canine IgG (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical use for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000.
Reactivities Canine
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgG (Fc specific) Goat Polyclonal Antibody
AP31540SU-N

USD 270.00

2 Weeks

Canine IgG (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in dog serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgG from serum or plasma.
Directions for use:
In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Reactivities Canine
Immunogen Highly purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgG (H+L chain) Goat Polyclonal Antibody
AP31541SU-N

USD 270.00

2 Weeks

Canine IgG (H+L chain) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal dog serum; to prepare an immunoadsorbent for the production of Ig-free dog serum, plasma or non-Ig preparations.
Directions for use:
In immunoelectrophoresis use 2 ml or equivalent against 120 ml antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ml antiserum in a 3 mm diameter centre well and 2 ml serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Canine
Immunogen Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgM (Fc specific) Goat Polyclonal Antibody
AP31542BT-N

USD 550.00

2 Weeks

Canine IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/4000.
Reactivities Canine
Conjugation Biotin
Immunogen Purified normal IgM isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgM (Fc specific) Goat Polyclonal Antibody
AP31542SU-N

USD 270.00

2 Weeks

Canine IgM (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in dog serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgM from serum or plasma.
Directions for use:
In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl in a 3 mm diameter center well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Reactivities Canine
Immunogen Highly purified normal IgM isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543SU-N

USD 270.00

2 Weeks

Mouse IgM (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgM from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Directions for use:
In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum.
In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Reactivities Mouse
Immunogen Highly purified homogenous IgM isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody
AP31795AP-N

USD 230.00

2 Weeks

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP

Applications ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate.
Western Blot (1/1000).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA.
Reactivities Chicken
Conjugation AP
Immunogen Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.
Mouse IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP32278GL-N

USD 200.00

3 Days

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, BGAL

Applications ELISA: 1/500.
Immunohistochemistry.
Reactivities Mouse
Conjugation BGAL
Immunogen Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins.
Porcine IgM (Fc specific) Goat Polyclonal Antibody
AP33049SU-N

USD 270.00

2 Weeks

Porcine IgM (Fc specific) goat polyclonal antibody, Serum

Applications In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in swine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgM from serum or plasma.
Recommended Working Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Precipitin titre: 1/32 when tested against pooled normal Swine serum in agar-block immunodiffusion titration.
Reactivities Porcine
Immunogen Highly purified normal IgM isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Hamster IgG (H+L chain), adsorbed Goat Polyclonal Antibody
R1326P

USD 225.00

3 Days

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:800,000. 
Western Blot: 1:5,000 - 1:50,000.
Immunohistochemistry: 1:1,000 - 1:5,000.
Immunogen Hamster IgG whole molecule.
Monkey IgM (Mu chain specific) Goat Polyclonal Antibody
R1345F

USD 295.00

3 Days

Monkey IgM (Mu chain specific) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Monkey IgM µ mu chain.
Monkey IgM (Mu chain specific) Goat Polyclonal Antibody
R1345T

USD 280.00

3 Days

Monkey IgM (Mu chain specific) goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Monkey IgM µ mu chain.
Monkey IgM (Mu chain specific) Goat Polyclonal Antibody
R1345TR

USD 295.00

3 Days

Monkey IgM (Mu chain specific) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
FLISA: 1:10,000-1:50,000
IF: 1:1,000-1:5,000
Conjugation Texas Red
Immunogen Monkey IgM µ (mu) heavy chain.nm
Monkey IgA (alpha chain specific) Goat Polyclonal Antibody
R1346F

USD 265.00

3 Days

Monkey IgA (alpha chain specific) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
FLISA: 1:10,000-1:50,000
IF: 1:1,000-1:5,000
Conjugation FITC
Immunogen Monkey IgA α chain.
Mouse IgA (+IgG +IgM) Goat Polyclonal Antibody
R1349HRP

USD 325.00

3 Days

Mouse IgA (+IgG +IgM) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/500-1/3,000 for Western blot), ELISA (1/100,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/250-1/1,500) as well as other Peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Conjugation HRP
Immunogen Mouse IgG, IgA and IgM whole molecules.
Rat IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
R1377B

USD 315.00

2 Weeks

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/150,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/10,000.
Conjugation Biotin
Immunogen Rat IgG, IgA and IgM whole molecules.
Rat IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
R1377TR

USD 315.00

3 Days

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:2,000-1:10,000.
Western Blot: 1:500-1:2,500.
Immunohistochemistry: 1:200-1:1,000.
Conjugation Texas Red
Immunogen Rat IgG, IgA and IgM whole molecules.
Human IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
R1397R

USD 415.00

3 Days

Human IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1/100-1/250) and Flow Cytometry or FACS analysis (1/100-1/250) as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
Conjugation PE
Immunogen Human IgG F(c) fragment.
Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
R1406AP

USD 325.00

3 Days

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/5,000 of the reconstitution concentration is suggested for this product. 
Recommended Dilutions: 
ELISA: 1/2000-1/10000. 
Western Blot: 1/500-1/2500. 
Immunochemistry: 1/200-1/1000.
Reactivities Mouse
Conjugation AP
Immunogen Mouse IgG F(c) fragment.
Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
R1406B

USD 275.00

3 Days

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin
Immunogen Mouse IgG F(c) fragment.
Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
R1406F

USD 300.00

3 Days

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Applications
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.  
Recommended Dilutions:
FLISA: 1/10,000-1/50,000. 
IF Microscopy: 1/1,000-1/5,000. 
Flow Cytometry: 1/500-1/2,500. 

 
Reactivities Mouse
Conjugation FITC
Immunogen Mouse IgG F(c) fragment.
Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
R1406HRP

USD 415.00

3 Days

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP

Applications ELISA: 1/20000. 
Western Blot: 1/500-1/2000.
Immunohistochemistry: 1/250-1/1000.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG F(c) fragment.
Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
R1406P

USD 240.00

3 Days

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. 
Recommended Dilutions:
ELISA: 1/10,000. 
Western Blot : 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Immunogen Mouse IgG F(c) fragment.
Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
R1406TR

USD 350.00

3 Days

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Texas Red

Applications This product is designed for Immunofluorescence Microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000. 
Immunofluorescence: 1/1,000-1/5,000. 
Flow Cytometry: 1/500-1/2,500.
Reactivities Mouse
Conjugation Texas Red
Immunogen Mouse IgG F(c) fragment.
Mouse IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
R1407R

USD 415.00

3 Days

Mouse IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1/100-1/250) and Flow Cytometry (1/100-1/250) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.
Conjugation PE
Immunogen Mouse IgG F(ab')2 fragment.
Mouse IgM (Mu chain specific), F(ab)2 Fragment Goat Polyclonal Antibody
R1408HRP

USD 265.00

3 Days

Mouse IgM (Mu chain specific), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. 
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Mouse IgM µ heavy chain
Mouse IgA (H+L chain), F(ab)2 Fragment Goat Polyclonal Antibody
R1409HRP

USD 240.00

3 Days

Mouse IgA (H+L chain), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000-1/5,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Mouse IgG, IgA and IgM whole molecules.
Rabbit IgG (H+L chain) F(ab)2 Fragment Goat Polyclonal Antibody
R1410F

USD 245.00

3 Days

Rabbit IgG (H+L chain) F(ab)2 Fragment goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA:  1/1000 - 1/50,000
IF Microscopy:  1/1000 - 1/5000
Conjugation FITC
Immunogen Rabbit IgG whole molecule.
Rabbit IgG (F(ab)2 specific adsorbed F(ab)2 Fragment) Goat Polyclonal Antibody
R1412HRP

USD 290.00

3 Days

Rabbit IgG (F(ab)2 specific adsorbed F(ab)2 Fragment) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer andspecificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Rabbit IgG F(ab')2 fragment.
Bovine IgG (H+L chain) Goat Polyclonal Antibody
R1616TR

USD 220.00

3 Days

Bovine IgG (H+L chain) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Fow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation Texas Red
Immunogen Bovine IgG whole molecule.
Mouse IgM (Mu chain specific), adsorbed Goat Polyclonal Antibody
SP1018HRP

USD 300.00

5 Days

Mouse IgM (Mu chain specific), adsorbed goat polyclonal antibody, HRP

Applications ELISA (1/1000).
Immunohistochemistry on Frozen and Paraffin Tissues (1/50).
Reactivities Mouse
Conjugation HRP
Immunogen Monoclonal Mouse IgM
Rat IgG (H+L chain, adsorbed) Goat Polyclonal Antibody
SP1020HRP

USD 330.00

5 Days

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Applications ELISA: 1/1000.
Immunohistochemistry on Frozen Sections: 1/50.
Immunohistochemistry on Paraffin Sections.
Reactivities Rat
Conjugation HRP
Immunogen Rat IgG