Secondary Antibodies

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Peroxidase Conjugated Goat Anti-Rabbit IgG (Min Hu, Ms)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rabbit IgG
Conjugation HRP
Immunogen Rabbit IgG, whole molecule

Peroxidase Conjugated Affinity Purified Goat anti-Mouse IgG [H&L]

Applications WB: 1: 1,000; ICC: 1:200; IP: 1:200
Reactivities Mouse IgG
Conjugation HRP
Immunogen Mouse IgG, whole molecule

Peroxidase Conjugated Goat Anti-Rabbit IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rabbit IgG
Conjugation HRP
Immunogen Rabbit IgG, whole molecule

Goat Anti-Rabbit IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Rabbit IgG
Conjugation Unconjugated
Immunogen Rabbit IgG, whole molecule

Peroxidase Conjugated Goat Anti-Mouse IgG (gamma-chain specific)

Applications Dot blot:0.5-1µg/ml.Western blot(DAB):0.7-3.3µg/ml.Western blot(ECL):0.2-0.5µg/ml.ELISA:0.2-0.3µg/ml.
Reactivities Mouse IgG
Conjugation HRP
Immunogen Mouse IgG, whole molecule

Goat Anti-Mouse IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Mouse IgG
Conjugation Unconjugated
Immunogen Mouse IgG, whole molecule

FITC Conjugated Goat Anti-Rabbit IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Rabbit IgG
Conjugation FITC
Immunogen Rabbit IgG, whole molecule

Biotin Conjugated Goat Anti-Rabbit IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Rabbit IgG
Conjugation Biotin
Immunogen Rabbit IgG, whole molecule

Rabbit Anti-Mouse IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Mouse IgG
Conjugation Unconjugated
Immunogen Mouse IgG, whole molecule

Peroxidase Conjugated Goat Anti-Mouse IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Mouse IgG
Conjugation HRP
Immunogen Mouse IgG, whole molecule

Peroxidase Conjugated Goat Anti-Rabbit IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rabbit IgG
Conjugation HRP
Immunogen Rabbit IgG, whole molecule

FITC Conjugated Goat Anti-Mouse IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Mouse IgG
Conjugation FITC
Immunogen Mouse IgG, whole molecule

Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Goat IgG
Conjugation HRP
Immunogen Goat IgG, whole molecule

Rabbit IgG (H+L chain) goat polyclonal antibody, HRP, Lyophilized

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/150,000-1/250,000.
Immunohistochemistry: 1/500-1/2,500.
Western blot: 1/10,000-1/40,000.
Reactivities Rabbit
Conjugation HRP
Immunogen Rabbit IgG, whole molecule

FITC Conjugated Goat Anti-Rabbit IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Rabbit IgG
Conjugation FITC
Immunogen Rabbit IgG, whole molecule

Biotin Conjugated Goat Anti-Mouse IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Mouse IgG
Conjugation Biotin
Immunogen Mouse IgG, whole molecule

Rabbit Anti-Goat IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Goat IgG
Conjugation Unconjugated
Immunogen Goat IgG, whole molecule

Biotin Conjugated Goat Anti-Rabbit IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Rabbit IgG
Conjugation Biotin
Immunogen Rabbit IgG, whole molecule

Rabbit IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions
IF Microscopy.
ELISA: 1/10,000-1/60,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2500.
Immunoprecipitation.
Reactivities Rabbit
Immunogen Rabbit IgG whole molecule

Monkey IgG (Fc specific) goat polyclonal antibody, FITC

Applications Can be used:
• In direct staining of cytoplasmic IgG in fixed Monkey cells and tissue substrates.
• To identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases.
• To identify a specific antigen or immune complex using a reference antibody of Monkey in the middle layer of the test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilution: 1/20-1/80.
Reactivities Monkey
Conjugation FITC
Immunogen Purified normal IgG isolated from pooled Rhesus Monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgG (H+L chain) sheep polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Immunogen Mouse IgG whole molecule.

Cy3 Conjugated Goat Anti-Rabbit IgG

Applications IF/ICC: 1:50-100; IHC: 1:50-100
Reactivities Rabbit IgG
Conjugation Cy3
Immunogen Rabbit IgG, whole molecule

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, FITC

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. It is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Conjugation FITC
Immunogen Sheep IgG F(ab')2 fragment.

Human IgM (Mu chain specific) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/80,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Human IgM µ (mu) heavy chain

Human IgG (Fc specific) goat polyclonal antibody, HRP

Applications ELISA: 1/3000.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation HRP
Immunogen Human IgG

Human IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1-5 µg/ml.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Immunogen Human IgG

Human IgG3 sheep polyclonal antibody, HRP

Applications ELISA.
Immunohistochemistry on Frozen Sections: 1/100-1/200.
Reactivities Human
Conjugation HRP
Immunogen Human IgG3 (kappa & lambda) purified from Human myeloma serum

Mouse IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for ELISA, Immunofluorescence Microscopy.
Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1000-1/5000.
Conjugation FITC
Immunogen Mouse IgG whole molecule.

Mouse IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000. 
Western blot: 1/2,000-1/10,000. 
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Immunogen Anti-Mouse IgG F(c) fragment was produced by repeated immunization with Mouse IgG F(c) fragment in goat.

Cy3 Conjugated Goat Anti-Mouse IgG

Applications IF/ICC: 1:50-100; IHC: 1:50-100
Reactivities Mouse IgG
Conjugation Cy3
Immunogen Mouse IgG, whole molecule

Rabbit IgG (H+L chain) goat polyclonal antibody, AP, Liquid

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/3,000-1/25,000.
Western blot: 1/1,000-1/4,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Rabbit
Conjugation AP
Immunogen Rabbit IgG, whole molecule.

Biotin Conjugated Goat Anti-Mouse IgM

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Mouse IgM
Conjugation Biotin
Immunogen Mouse IgM, whole molecule

Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
For use in ELISA a dilution of 1:3,000-1:12,000 is recommended.
Immunogen Mouse IgG whole molecule.

Biotin Conjugated Rabbit Anti-Goat IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Goat IgG
Conjugation Biotin
Immunogen Goat IgG, whole molecule

Mouse IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA : 1:50,000 - 1:300,000
Western Blot : 1:10,000 - 1:30,000
Immunohistochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Conjugation HRP
Immunogen Mouse IgG whole molecule.

Mouse IgG (H+L chain) sheep polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/200,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG whole molecule.

Human IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/130,000
Western blot: 1/1,000-1/5,000
Immunohistochemistry: 1/500-1/2,500
Conjugation HRP
Immunogen Human IgG F(c) fragment.

Human IgE goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Human, Monkey
Conjugation HRP
Immunogen Native Human IgE

Mouse IgD (Fc specific) goat polyclonal antibody, Biotin

Applications In Immunocytochemical and Immunohistochemical staining of IgD at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgD antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgD isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgD in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/200-1/1000.
Reactivities Mouse
Conjugation Biotin
Immunogen Purified polyclonal and monoclonal IgD isolated from BALB/C and C57BL Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining to identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/5000 - 1/20000.
Reactivities Rabbit
Conjugation Biotin
Immunogen Purified normal IgG, IgA and IgM isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Goat IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA: 1/50,000-1/100,000. 
Western Blot: 1/5,000-1/20,000. 
Immunohistochemistry: 1/1,000-1/5,000. 
Note: HRP Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Conjugation HRP
Immunogen Goat IgG whole molecule.

Rabbit IgG (H+L chain) goat polyclonal antibody, FITC, Lyophilized

Applications This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Immunofluoresence: 1/1,000-1/5,000.
Fluorescent Western Blot.
Reactivities Rabbit
Conjugation FITC
Immunogen Rabbit IgG, whole molecule.

Mouse IgG (Fc specific) goat polyclonal antibody, FITC

Applications This product is designed for Immunofluorescence microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA: 1/10,000-1/50,000.
Western blot: 1/1,000-1/5,000.
Immunofluorescence: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation FITC
Immunogen Mouse IgG F(c) fragment.

FITC Conjugated Goat Anti-Mouse IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Mouse IgG
Conjugation FITC
Immunogen Mouse IgG, whole molecule

Rabbit Anti-Human IgG Secondary Antibody

Applications WB, IHC, IF/ICC, ELISA
Reactivities Human IgG
Conjugation Unconjugated
Immunogen Human IgG, whole molecule

Monkey IgG (Fab specific) rabbit polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
Reactivities Monkey
Conjugation FITC
Immunogen Purified normal Fab of monkey polyclonal IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1/100,000.
Western blot: 1/500-1/3,000.
Immunohistochemistry: 1/500-1/1,500.
Conjugation HRP
Immunogen Rat IgG, IgA, IgM whole molecules

Cy3 Conjugated Rabbit Anti-Goat IgG

Applications IF/ICC: 1:50-100; IHC: 1:50-100
Reactivities Goat IgG
Conjugation Cy3
Immunogen Goat IgG, whole molecule

Mouse IgE (Fc specific) goat polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
depending on the method used.
Reactivities Mouse
Conjugation HRP
Immunogen Purified homogenous IgE isolated from pooled Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids).
Immunocytochemistry.
Immunohistochemistry.
Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties.
Dot blot.
Western blot.
General Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen.
Reactivities Rabbit
Conjugation HRP
Immunogen Purified immunoglobulin fractions isolated from pooled rabbit serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.