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Peroxidase Conjugated Goat Anti-Rabbit IgG (Min Hu, Ms)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Rabbit IgG |
| Conjugation | HRP |
| Immunogen | Rabbit IgG, whole molecule |
Products
Peroxidase Conjugated Affinity Purified Goat anti-Mouse IgG [H&L]
| Applications | WB: 1: 1,000; ICC: 1:200; IP: 1:200 |
| Reactivities | Mouse IgG |
| Conjugation | HRP |
| Immunogen | Mouse IgG, whole molecule |
Peroxidase Conjugated Goat Anti-Rabbit IgG (gamma-chain specific)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Rabbit IgG |
| Conjugation | HRP |
| Immunogen | Rabbit IgG, whole molecule |
Peroxidase Conjugated Goat Anti-Mouse IgG (gamma-chain specific)
| Applications | Dot blot:0.5-1µg/ml.Western blot(DAB):0.7-3.3µg/ml.Western blot(ECL):0.2-0.5µg/ml.ELISA:0.2-0.3µg/ml. |
| Reactivities | Mouse IgG |
| Conjugation | HRP |
| Immunogen | Mouse IgG, whole molecule |
USD 260.00
5 Days
Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), HRP, Purified
| Applications | ELISA: 1/4000-1/8000 (See also Ref.2-9). Applications Reported in Literature: Flow Cytometry (Ref.15,18,19). Immunohistochemistry on Frozen Sections (Ref.10). Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11). Immunocytochemistry (Ref.12). Immunoblotting (Ref.3,13-16). Microarray (Ref.17). Multiplex (Ref.2,20-22). Purification (Ref.15). |
| Reactivities | Human |
| Conjugation | HRP |
Peroxidase Conjugated Goat Anti-Mouse IgG (gamma-chain specific)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Mouse IgG |
| Conjugation | HRP |
| Immunogen | Mouse IgG, whole molecule |
Peroxidase Conjugated Goat Anti-Rabbit IgG (gamma-chain specific)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Rabbit IgG |
| Conjugation | HRP |
| Immunogen | Rabbit IgG, whole molecule |
Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Goat IgG |
| Conjugation | HRP |
| Immunogen | Goat IgG, whole molecule |
Rabbit IgG (H+L chain) goat polyclonal antibody, HRP, Lyophilized
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/150,000-1/250,000. Immunohistochemistry: 1/500-1/2,500. Western blot: 1/10,000-1/40,000. |
| Reactivities | Rabbit |
| Conjugation | HRP |
| Immunogen | Rabbit IgG, whole molecule |
USD 680.00
2 Weeks
Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), HRP
| Applications | To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting. General Recommended Dilutions: ELISA: 1/20-1/500. Western Blot: 1/40-1/1000. Histochemical Use: 1/5-1/20. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, HRP
| Applications | ELISA (both capture and detection). Western Blot. Radioimmunoassay. |
| Reactivities | Human |
| Conjugation | HRP |
USD 1,340.00
2 Weeks
Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, HRP, Purified
| Applications | Immunoassay. ELISA: good binding on plastic. Immunohistochemistry on Frozen Sections. |
| Reactivities | Mouse |
| Conjugation | HRP |
Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, HRP
| Applications | ELISA: 1/2,000-1/8,000. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgM (Mu chain specific) goat polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions ELISA: 1/80,000. Western blot: 1/2,000-1/20,000. Immunohistochemistry: 1/500-1/2,500. |
| Conjugation | HRP |
| Immunogen | Human IgM µ (mu) heavy chain |
Human IgG (Fc specific) goat polyclonal antibody, HRP
| Applications | ELISA: 1/3000. Immunohistochemistry on Frozen Sections. |
| Reactivities | Human |
| Conjugation | HRP |
| Immunogen | Human IgG |
Human IgG3 sheep polyclonal antibody, HRP
| Applications | ELISA. Immunohistochemistry on Frozen Sections: 1/100-1/200. |
| Reactivities | Human |
| Conjugation | HRP |
| Immunogen | Human IgG3 (kappa & lambda) purified from Human myeloma serum |
Mouse IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA : 1:50,000 - 1:300,000 Western Blot : 1:10,000 - 1:30,000 Immunohistochemistry: 1:500 - 1:2,500 Other Dilution: User Optimized |
| Conjugation | HRP |
| Immunogen | Mouse IgG whole molecule. |
Mouse IgG (H+L chain) sheep polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/200,000 Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Mouse IgG whole molecule. |
USD 280.00
2 Weeks
Human IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/130,000 Western blot: 1/1,000-1/5,000 Immunohistochemistry: 1/500-1/2,500 |
| Conjugation | HRP |
| Immunogen | Human IgG F(c) fragment. |
Human IgE goat polyclonal antibody, HRP
| Applications | ELISA: 1/10000-1/100000. |
| Reactivities | Human, Monkey |
| Conjugation | HRP |
| Immunogen | Native Human IgE |
Goat IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/50,000-1/100,000. Western Blot: 1/5,000-1/20,000. Immunohistochemistry: 1/1,000-1/5,000. Note: HRP Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. |
| Conjugation | HRP |
| Immunogen | Goat IgG whole molecule. |
Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, HRP
| Applications | ELISA: 1/100,000. Western blot: 1/500-1/3,000. Immunohistochemistry: 1/500-1/1,500. |
| Conjugation | HRP |
| Immunogen | Rat IgG, IgA, IgM whole molecules |
Mouse IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. depending on the method used. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Purified homogenous IgE isolated from pooled Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP
| Applications | ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids). Immunocytochemistry. Immunohistochemistry. Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties. Dot blot. Western blot. General Recommended Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen. |
| Reactivities | Rabbit |
| Conjugation | HRP |
| Immunogen | Purified immunoglobulin fractions isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgA + IgG + IgM goat polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western blot: 1/2,000-1/10,000. Immunoprecipitation: 1/1,000-1/5,000. |
| Reactivities | Human |
| Conjugation | HRP |
| Immunogen | Human IgG, IgA and IgM whole molecules in goat. |
Mouse IgG (Fc specific) goat polyclonal antibody, HRP
| Applications | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000 Western blot: 1/1,000-1/5,000 Flow Cytometry: 1/500-1/2,500 |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Mouse IgG F(c) fragment |
Peroxidase Conjugated Rabbit Anti-Rat IgG (gamma-chain specific)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Rat IgG |
| Conjugation | HRP |
| Immunogen | Rat IgG, whole molecule |
Human IgA (Secretory component) goat polyclonal antibody, HRP
| Applications | In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in human milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/6000. |
| Reactivities | Human |
| Conjugation | HRP |
| Immunogen | Secretory component can be present in Human secretions bound to secretory IgA (sIgA) and in free form. Free Human secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgG (Fab specific) rabbit polyclonal antibody, HRP
| Applications | Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommneded Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/10000. |
| Reactivities | Monkey |
| Conjugation | HRP |
| Immunogen | Purified normal Fab of polyclonal Rhesus Monkey IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. Note: This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/40,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Rabbit IgG whole molecule. |
USD 680.00
2 Weeks
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), HRP
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: from 1/20 upwards. ELISA: form 1/50 upwards. Western blot: from 1/100 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
Duck IgM (Fc specific) goat polyclonal antibody, HRP
| Applications | Suitable for use in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of Duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in duck serum or other body fluids. Recommended Dilutions: ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/3,000. Immunohistochemistry and Cytochemistry: 1/50-1/250. Note: This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Duck |
| Conjugation | HRP |
| Immunogen | Purified normal IgM isolated from pooled Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To demonstrate circulating IgE antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using an reference antibody of Human origin known to be of the IgE isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgE in Human serum or other body fluids. Recommneded Dilutions: Histochemistry and Cytochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/25,000. |
| Reactivities | Human |
| Conjugation | HRP |
| Immunogen | Purified monoclonal IgE isolated from Human serum. Feund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgG (Fc specific) goat polyclonal antibody, HRP
| Applications | Dot blot. Immunoblotting. ELISA. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. This antibody can be used: In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG in monkey serum or other body fluids. This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histo- and Cytochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/20000. |
| Reactivities | Monkey |
| Conjugation | HRP |
| Immunogen | Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgA + IgG + IgM goat polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. |
| Conjugation | HRP |
| Immunogen | Monkey IgG IgA and IgM whole molecules in goat. |
Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, HRP
| Applications | ELISA: 1/4,000-1/10,000. Western blotting. Dot- and Slot-immunoblotting. Immunohistochemistry on Frozen Sections: 1/10-1/20. |
| Reactivities | Human |
| Conjugation | HRP |
Porcine IgA goat polyclonal antibody, HRP
| Applications | ELISA: 1/10,000-1/100,000. |
| Reactivities | Porcine |
| Conjugation | HRP |
| Immunogen | Purified Porcine IgA |
USD 680.00
2 Weeks
Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), HRP
| Applications | To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting. General Recommended Dilutions: Histochemical Use: 1/10-1/100. ELISA: from 1/100 upwards. Western blot: from 1/250 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
Chicken IgM (Fc specific) goat polyclonal antibody, HRP
| Applications | Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in chicken serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. |
| Reactivities | Chicken |
| Conjugation | HRP |
| Immunogen | Purified normal IgM isolated from pooled chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea Pig IgG2 (subclass specific) goat polyclonal antibody, HRP
| Applications | This antibody can be used in Enzyme Immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: Histochemical and Cytochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,0000. |
| Reactivities | Guinea Pig |
| Conjugation | HRP |
| Immunogen | Purified IgG2 isolated from Guinea Pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea Pig IgM (Fc specific) goat polyclonal antibody, HRP
| Applications | Can be used for Enzyme-Immunocytochemical and immunohistochemical detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Guinea Pig origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Guinea Pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/400-1/4000. |
| Reactivities | Guinea Pig |
| Conjugation | HRP |
| Immunogen | Purified normal IgM isolated from pooled Guinea Pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgD (Fc specific) goat polyclonal antibody, HRP
| Applications | Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgD antibodies in serodiagnostic microbiology and autoimmune diseases; in nonisotopic assay methodology (e.g. ELISA) to measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/50 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/10,000. |
| Reactivities | Human |
| Conjugation | HRP |
| Immunogen | Purified polyclonal IgD isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rat IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | This antibody can be used In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: Histochemistry and Cytochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. |
| Reactivities | Rat |
| Conjugation | HRP |
| Immunogen | Purified homogenous IgE isolated from Rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgE (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Purified homogenous IgE isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rabbit IgM (Fc specific) goat polyclonal antibody, HRP
| Applications | Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in rabbit serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000. |
| Reactivities | Rabbit |
| Conjugation | HRP |
| Immunogen | Purified normal IgM isolated from pooled Rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rabbit IgA (Fc specific) goat polyclonal antibody, HRP
| Applications | Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in rabbit serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/50 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Rabbit |
| Conjugation | HRP |
| Immunogen | Purified normal IgA isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rat IgG2b (subclass specific) goat polyclonal antibody, HRP
| Applications | Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in Rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000. |
| Reactivities | Rat |
| Conjugation | HRP |
| Immunogen | Pools of purified homogenous IgG2b isolated from Rat sera. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rat IgG2ab (subclass specific) goat polyclonal antibody, HRP
| Applications | Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000 - 1/10,000. |
| Reactivities | Rat |
| Conjugation | HRP |
| Immunogen | Pools of purified homogenous IgG2a and IgG2b isolated from rat sera. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgG3 (subclass specific) goat polyclonal antibody, HRP
| Applications | This antibody can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates To demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in Mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: Histochemical/Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/7,000 depending on the method used. Working dilutions schould be stored at +4°C, not refrozen, and preferably used the same day. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Pools of purified homogenous IgG3 isolated from pooled Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Bovine IgM (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids. Recommneded Dilutions: Histochemistry and Cytochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000. |
| Reactivities | Bovine |
| Conjugation | HRP |
| Immunogen | Purified normal IgM isolated from pooled Bovine serum. Feund’s complete adjuvant is used in the first step of the immunization procedure. |
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