UL8 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100795

Myc-DDK-tagged ORF clone of viral ORF for helicase-primase subunit [Human herpesvirus 1], codon optimized for human cell expression, NP_044609

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100795 represents NCBI reference of NP_044609 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACCGCTGATATTGTGTGGGTCGAGGAGTCCGTTAGCGCTATTACCCTTTATGCTGTCTGGCTCC
  CTCCTAGAGCTCGCGAGTACTTTCACGCCCTGGTGTATTTCGTGTGCCGCAACGCCGCTGGGGAGGGACG
  CGCCCGGTTCGCTGAGGTTTCCGTGACCGCCACCGAGCTCAGGGACTTTTACGGGTCTGCAGATGTGAGT
  GTGCAAGCTGTTGTTGCCGCTGCTCGCGCGGCCACTACCCCCGCTGCATCACCCCTTGAGCCACTTGAGA
  ACCCCACTTTGTGGCGAGCGCTTTACGCATGCGTCCTGGCCGCACTCGAGCGGCAGACCGGCCCAGTGGC
  ACTGTTTGCACCTTTGAGAATTGGAAGTGATCCACGGACTGGGCTTGTTGTCAAAGTGGAGCGCGCATCC
  TGGGGGCCGCCAGCAGCCCCTCGGGCAGCCCTCCTTGTAGCCGAAGCTAATATTGACATTGACCCAATGG
  CCCTTGCTGCCAGGGTAGCCGAGCACCCCGATGCGCGCCTGGCCTGGGCTAGATTGGCCGCCATTCGGGA
  CACTCCGCAGTGTGCTTCAGCTGCTTCCCTGACCGTCAATATAACCACTGGGACAGCTTTGTTCGCACGA
  GAATATCAGACTCTCGCTTTTCCCCCCATTAAGAAGGAGGGTGCCTTTGGCGACCTTGTCGAAGTGTGTG
  AAGTGGGCCTGCGACCCCGCGGTCACCCACAGCGCGTGACCGCCCGAGTCCTCCTGCCCCGCGACTATGA
  CTACTTCGTTAGTGCCGGCGAAAAGTTTAGTGCTCCAGCTCTTGTGGCTCTGTTCCGGCAGTGGCACACC
  ACCGTACATGCGGCTCCAGGCGCACTTGCTCCCGTGTTCGCCTTTCTCGGCCCAGAATTCGAAGTGAGGG
  GGGGACCCGTGCCATATTTTGCTGTGCTGGGCTTTCCTGGGTGGCCCACATTCACTGTTCCTGCAACTGC
  CGAAAGCGCGAGAGATCTGGTGAGGGGCGCCGCGGCTGCTTACGCAGCACTTCTGGGTGCATGGCCAGCG
  GTGGGTGCCCGGGTAGTTCTTCCTCCCCGCGCATGGCCAGGTGTGGCATCAGCCGCAGCCGGCTGTCTTC
  TGCCAGCCGTGAGAGAGGCAGTCGCACGATGGCACCCCGCCACAAAGATCATACAGCTCTTGGACCCACC
  TGCCGCCGTGGGGCCTGTGTGGACAGCCAGGTTCTGCTTCCCTGGTCTGCGCGCTCAGCTCCTTGCTGCG
  TTGGCAGACCTCGGTGGAAGCGGGCTGGCAGATCCACACGGGAGGACTGGTTTGGCTAGACTGGACGCAC
  TTGTGGTAGCCGCGCCATCTGAGCCTTGGGCTGGTGCAGTGCTGGAGAGACTGGTCCCCGACACCTGTAA
  TGCCTGCCCCGCTTTGAGGCAGCTGCTGGGTGGAGTTATGGCGGCTGTGTGCCTCCAGATTGAGGAAACC
  GCTAGCTCTGTGAAATTTGCCGTGTGTGGCGGGGACGGGGGCGCGTTTTGGGGCGTTTTTAATGTGGATC
  CGCAGGACGCAGATGCCGCCTCTGGCGTGATCGAAGATGCAAGGAGGGCGATCGAGACTGCGGTGGGAGC
  CGTGTTGAGAGCAAACGCTGTCAGACTTAGACATCCGCTGTGCCTTGCCCTGGAAGGCGTATATACCCAC
  GCAGTGGCCTGGAGCCAGGCAGGGGTTTGGTTTTGGAACTCAAGGGATAACACCGATCATCTTGGCGGGT
  TCCCGCTCCGAGGACCCGCATATACAACTGCTGCCGGAGTGGTTAGAGACACACTGAGGCGAGTTCTCGG
  CCTGACCACAGCCTGCGTGCCCGAAGAGGATGCACTTACTGCCAGGGGACTGATGGAGGATGCTTGTGAT
  CGGCTGATCCTCGATGCTTTCAATAAACGGTTGGACGCTGAGTACTGGTCCGTCCGAGTCTCTCCATTCG
  AGGCCTCTGATCCTCTTCCACCTACTGCTTTCAGAGGCGGCGCGCTTCTGGATGCGGAGCATTATTGGCG
  CAGAGTCGTCCGAGTCTGTCCTGGAGGCGGTGAGTCAGTGGGCGTGCCAGTCGATCTCTATCCTCGCCCA
  CTGGTGTTGCCCCCAGTGGACTGCGCCCATCACTTGAGAGAGATTTTGAGAGAGATCGAACTGGTCTTCA
  CCGGAGTGCTTGCCGGTGTTTGGGGGGAGGGTGGAAAATTCGTTTACCCCTTTGACGACAAAATGAGTTT
  TCTCTTTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100795 representing NP_044609
  Red=Cloning sites Green=Tags
  
  MDTADIVWVEESVSAITLYAVWLPPRAREYFHALVYFVCRNAAGEGRARFAEVSVTATELRDFYGSADVS
  VQAVVAAARAATTPAASPLEPLENPTLWRALYACVLAALERQTGPVALFAPLRIGSDPRTGLVVKVERAS
  WGPPAAPRAALLVAEANIDIDPMALAARVAEHPDARLAWARLAAIRDTPQCASAASLTVNITTGTALFAR
  EYQTLAFPPIKKEGAFGDLVEVCEVGLRPRGHPQRVTARVLLPRDYDYFVSAGEKFSAPALVALFRQWHT
  TVHAAPGALAPVFAFLGPEFEVRGGPVPYFAVLGFPGWPTFTVPATAESARDLVRGAAAAYAALLGAWPA
  VGARVVLPPRAWPGVASAAAGCLLPAVREAVARWHPATKIIQLLDPPAAVGPVWTARFCFPGLRAQLLAA
  LADLGGSGLADPHGRTGLARLDALVVAAPSEPWAGAVLERLVPDTCNACPALRQLLGGVMAAVCLQIEET
  ASSVKFAVCGGDGGAFWGVFNVDPQDADAASGVIEDARRAIETAVGAVLRANAVRLRHPLCLALEGVYTH
  AVAWSQAGVWFWNSRDNTDHLGGFPLRGPAYTTAAGVVRDTLRRVLGLTTACVPEEDALTARGLMEDACD
  RLILDAFNKRLDAEYWSVRVSPFEASDPLPPTAFRGGALLDAEHYWRRVVRVCPGGGESVGVPVDLYPRP
  LVLPPVDCAHHLREILREIELVFTGVLAGVWGEGGKFVYPFDDKMSFLFA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001806
• ORF Size: 2250 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001806.1, NP_044609
• RefSeq ORF: 2250
• Locus ID: 2703432
• MW: 79.9 kDa