US6 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100854

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein D [Human herpesvirus 1], codon optimized for human cell expression, NP_044668

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100854 represents NCBI reference of NP_044668 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGGCGCAGCCGCCCGGCTGGGGGCCGTGATTTTGTTTGTAGTCATAGTAGGGCTGCACGGCGTAA
  GATCTAAGTACGCCCTTGTAGATGCTAGCCTCAAGATGGCAGATCCTAACAGGTTCAGAGGCAAGGACCT
  TCCGGTGCTCGACCAGTTGACCGACCCTCCAGGTGTAAGAAGAGTCTACCACATCCAGGCGGGGTTGCCC
  GACCCTTTCCAGCCCCCCTCCCTGCCAATCACAGTGTACTATGCCGTTCTTGAGCGGGCCTGCCGCAGCG
  TGTTGTTGAATGCCCCCAGTGAGGCTCCTCAGATTGTGCGCGGAGCGAGCGAAGACGTTAGAAAACAGCC
  TTATAACCTGACTATCGCCTGGTTCAGGATGGGAGGCAATTGCGCCATCCCCATTACAGTGATGGAATAC
  ACCGAATGTAGCTATAATAAGTCCCTCGGTGCCTGCCCAATTCGAACTCAGCCACGGTGGAATTATTATG
  ACTCATTTTCCGCCGTGAGTGAAGATAATCTTGGTTTCCTGATGCACGCGCCTGCGTTCGAGACGGCCGG
  AACCTATCTCCGACTCGTGAAAATCAATGACTGGACCGAAATTACCCAGTTCATCCTGGAGCACCGGGCC
  AAGGGTTCCTGCAAGTACGCCCTTCCCCTGCGGATACCCCCTTCTGCATGTCTTAGCCCTCAGGCCTACC
  AGCAGGGAGTGACAGTGGACTCAATTGGGATGCTCCCACGGTTCATACCTGAGAACCAGAGAACCGTGGC
  CGTGTACAGCCTGAAAATCGCAGGCTGGCATGGCCCAAAGGCCCCCTACACATCCACTCTCCTCCCCCCT
  GAGCTGTCCGAAACCCCCAATGCTACACAGCCTGAACTGGCGCCAGAGGACCCAGAAGACAGCGCACTGC
  TGGAGGACCCGGTAGGCACAGTGGCCCCCCAAATTCCCCCCAATTGGCATATCCCCTCTATCCAGGATGC
  GGCCACTCCGTACCACCCACCAGCCACTCCCAATAACATGGGCCTCATTGCTGGGGCCGTCGGAGGATCA
  CTCCTGGCGGCCCTGGTGATTTGTGGCATCGTATATTGGATGCGGAGGCACACCCAAAAAGCCCCCAAGA
  GAATAAGACTGCCCCACATAAGAGAGGACGACCAACCTTCTTCACACCAGCCACTGTTCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100854 representing NP_044668
  Red=Cloning sites Green=Tags
  
  MGGAAARLGAVILFVVIVGLHGVRSKYALVDASLKMADPNRFRGKDLPVLDQLTDPPGVRRVYHIQAGLP
  DPFQPPSLPITVYYAVLERACRSVLLNAPSEAPQIVRGASEDVRKQPYNLTIAWFRMGGNCAIPITVMEY
  TECSYNKSLGACPIRTQPRWNYYDSFSAVSEDNLGFLMHAPAFETAGTYLRLVKINDWTEITQFILEHRA
  KGSCKYALPLRIPPSACLSPQAYQQGVTVDSIGMLPRFIPENQRTVAVYSLKIAGWHGPKAPYTSTLLPP
  ELSETPNATQPELAPEDPEDSALLEDPVGTVAPQIPPNWHIPSIQDAATPYHPPATPNNMGLIAGAVGGS
  LLAALVICGIVYWMRRHTQKAPKRIRLPHIREDDQPSSHQPLFY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001806
• ORF Size: 1182 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001806.1, NP_044668
• RefSeq ORF: 1182
• Locus ID: 2703444
• MW: 43.3 kDa