UL48 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100836

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for transactivating tegument protein VP16 [Human herpesvirus 1], codon optimized for human cell expression, NP_044650


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Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol UL48
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100836 represents NCBI reference of NP_044650 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATTTGCTGGTGGACGAACTCTTCGCTGACATGAACGCTGATGGCGCTTCTCCGCCCCCACCACGGC
CCGCCGGCGGTCCTAAAAACACCCCTGCTGCACCTCCCCTCTATGCAACCGGGCGCCTGAGTCAGGCCCA
GCTGATGCCATCCCCACCCATGCCAGTTCCGCCCGCTGCTCTTTTCAATAGACTGCTGGACGACTTGGGC
TTTAGCGCAGGACCTGCCTTGTGTACCATGCTGGATACCTGGAACGAGGACCTGTTTTCCGCTCTCCCAA
CTAACGCCGACCTGTATAGAGAATGCAAATTTCTGAGCACACTTCCTAGTGATGTCGTCGAATGGGGAGA
TGCTTACGTCCCAGAGAGGACCCAGATCGACATACGCGCCCATGGAGACGTCGCATTCCCCACTCTGCCC
GCAACAAGGGACGGATTGGGCTTGTATTACGAAGCCCTTAGTAGATTCTTCCATGCAGAACTCCGAGCAC
GCGAAGAAAGCTACCGAACAGTTCTCGCCAACTTCTGTAGCGCCTTGTACCGGTATCTCAGAGCGTCAGT
CCGGCAATTGCACCGCCAGGCCCACATGCGGGGCAGAGATAGGGACCTGGGCGAAATGCTGCGCGCCACT
ATCGCTGACAGATACTACAGGGAGACCGCACGCCTCGCCAGGGTCCTGTTCTTGCACCTGTATCTGTTCC
TCACGAGAGAGATACTTTGGGCGGCATACGCCGAGCAAATGATGAGACCAGACCTGTTCGACTGCCTGTG
TTGCGATCTGGAGAGCTGGCGCCAGCTCGCCGGACTGTTCCAGCCTTTTATGTTCGTCAATGGGGCACTC
ACTGTGCGGGGAGTGCCTATTGAGGCGAGACGCCTGCGAGAACTGAATCACATTAGGGAGCACCTGAATC
TGCCCTTGGTTAGAAGTGCTGCCACCGAAGAACCGGGTGCCCCACTGACCACCCCACCAACCCTGCACGG
GAACCAGGCCAGAGCATCAGGCTACTTTATGGTGCTGATTCGCGCAAAACTTGACAGTTATAGCTCCTTT
ACAACCTCACCAAGCGAGGCCGTCATGCGGGAGCATGCATACAGTCGAGCACGGACGAAAAACAATTATG
GCAGTACAATTGAGGGATTGCTCGATCTGCCTGACGACGATGCCCCTGAGGAGGCCGGACTGGCTGCACC
AAGACTTTCCTTTCTCCCGGCTGGGCATACTCGGAGGTTGAGTACCGCACCCCCGACCGACGTCTCTCTG
GGCGACGAGTTGCACTTGGACGGTGAGGATGTGGCCATGGCACATGCCGACGCTCTTGACGATTTTGACC
TCGACATGCTGGGTGACGGGGACAGTCCTGGGCCTGGGTTCACTCCACACGATTCAGCCCCCTACGGCGC
TCTGGATATGGCTGACTTTGAGTTTGAACAGATGTTCACGGACGCACTGGGAATCGACGAGTATGGTGGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100836 representing NP_044650
Red=Cloning sites Green=Tags

MDLLVDELFADMNADGASPPPPRPAGGPKNTPAAPPLYATGRLSQAQLMPSPPMPVPPAALFNRLLDDLG
FSAGPALCTMLDTWNEDLFSALPTNADLYRECKFLSTLPSDVVEWGDAYVPERTQIDIRAHGDVAFPTLP
ATRDGLGLYYEALSRFFHAELRAREESYRTVLANFCSALYRYLRASVRQLHRQAHMRGRDRDLGEMLRAT
IADRYYRETARLARVLFLHLYLFLTREILWAAYAEQMMRPDLFDCLCCDLESWRQLAGLFQPFMFVNGAL
TVRGVPIEARRLRELNHIREHLNLPLVRSAATEEPGAPLTTPPTLHGNQARASGYFMVLIRAKLDSYSSF
TTSPSEAVMREHAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSL
GDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001806
ORF Size 1470 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_001806.1, NP_044650
RefSeq ORF 1470
Locus ID 2703416
MW 54.3 kDa

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