UL12 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100799

Myc-DDK-tagged ORF clone of viral ORF for deoxyribonuclease [Human herpesvirus 1], codon optimized for human cell expression, NP_044613

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100799 represents NCBI reference of NP_044613 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCAACCGTCGGTCCTGCCTGTCCTCCTGGCCGAACCGTGACCAAACGCCCATGGGCTCTGGCGG
  AGGATACACCACGAGGCCCAGATTCTCCTCCCAAGAGGCCAAGGCCAAACTCCCTGCCTCTTACCACTAC
  CTTTAGGCCGCTCCCCCCGCCCCCTCAAACCACATCTGCAGTTGACCCAAGTAGTCATAGCCCAGTGAAC
  CCCCCACGGGATCAGCATGCAACAGACACAGCCGACGAAAAACCTAGAGCCGCTTCACCTGCTCTGTCTG
  ATGCCAGCGGACCACCCACCCCCGACATCCCTCTCTCCCCTGGGGGCACCCACGCTAGGGATCCAGACGC
  CGATCCAGACAGCCCCGATTTGGACAGCATGTGGAGTGCCAGTGTGATCCCGAATGCACTGCCTTCACAC
  ATACTCGCCGAAACATTTGAGAGACATCTGAGGGGATTGCTCCGAGGAGTGAGGGCTCCACTGGCTATAG
  GCCCCTTGTGGGCGCGGTTGGACTACCTCTGTAGCCTCGCGGTTGTCCTTGAGGAGGCTGGCATGGTGGA
  TAGAGGGCTCGGGAGACACCTGTGGCGGTTGACAAGGCGCGGACCCCCAGCCGCAGCTGATGCGGTAGCG
  CCAAGGCCTCTCATGGGCTTCTACGAGGCCGCTACACAGAACCAGGCCGATTGCCAGCTGTGGGCTCTTC
  TGCGCAGGGGTCTCACCACAGCATCCACCCTGAGATGGGGTCCACAGGGACCTTGTTTCAGCCCGCAGTG
  GTTGAAGCACAATGCAAGCCTTCGCCCAGATGTTCAGAGTAGCGCAGTTATGTTCGGACGGGTTAACGAG
  CCTACAGCCAGGTCCTTGCTCTTTCGGTATTGCGTGGGGCGGGCCGATGACGGGGGTGAGGCCGGGGCCG
  ATACGCGCAGGTTCATATTCCACGAGCCCTCAGACCTGGCTGAAGAGAATGTTCACACATGTGGAGTTCT
  GATGGACGGTCATACCGGGATGGTTGGTGCCAGCCTCGACATCCTTGTGTGTCCCCGCGACATCCATGGG
  TACCTGGCACCCGTCCCCAAGACCCCACTGGCATTCTACGAAGTGAAATGCCGCGCTAAGTACGCCTTTG
  ACCCTATGGACCCAAGTGATCCTACCGCTAGTGCCTATGAGGACCTGATGGCTCACAGGTCTCCTGAAGC
  CTTTCGGGCCTTTATACGCTCAATTCCAAAACCTAGCGTAAGATACTTCGCCCCTGGAAGGGTGCCCGGC
  CCAGAAGAGGCCCTGGTTACACAGGACCAAGCGTGGTCCGAAGCACATGCTAGCGGCGAGAAGCGAAGAT
  GCTCTGCTGCCGATAGAGCGCTGGTGGAGTTGAACTCTGGCGTTGTGTCCGAGGTGCTGCTGTTCGGCGC
  TCCGGACCTGGGAAGGCACACTATCAGCCCCGTGAGCTGGTCTAGTGGAGATCTGGTTAGACGAGAGCCT
  GTTTTTGCCAACCCCAGACATCCTAACTTTAAACAGATTCTGGTGCAAGGTTACGTGCTGGACAGCCACT
  TCCCGGATTGTCCTCCACATCCTCATCTGGTGACCTTTATCGGACGGCACCGCACCAGCGCCGAGGAAGG
  CGTCACCTTCAGACTTGAGGATGGCGCCGGGGCTCTCGGCGCCGCAGGCCCGAGCAAGGCATCCATTCTG
  CCGAATCAGGCGGTCCCTATCGCTCTGATCATAACCCCCGTGCGGATCGACCCTGAGATCTACAAGGCCA
  TACAGAGGTCAAGTCGGCTTGCCTTCGACGACACCCTGGCCGAATTGTGGGCTTCTCGGAGTCCCGGACC
  CGGACCCGCCGCCGCCGAAACAACTTCTTCATCTCCCACCACAGGACGGAGCTCCCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100799 representing NP_044613
  Red=Cloning sites Green=Tags
  
  MESTVGPACPPGRTVTKRPWALAEDTPRGPDSPPKRPRPNSLPLTTTFRPLPPPPQTTSAVDPSSHSPVN
  PPRDQHATDTADEKPRAASPALSDASGPPTPDIPLSPGGTHARDPDADPDSPDLDSMWSASVIPNALPSH
  ILAETFERHLRGLLRGVRAPLAIGPLWARLDYLCSLAVVLEEAGMVDRGLGRHLWRLTRRGPPAAADAVA
  PRPLMGFYEAATQNQADCQLWALLRRGLTTASTLRWGPQGPCFSPQWLKHNASLRPDVQSSAVMFGRVNE
  PTARSLLFRYCVGRADDGGEAGADTRRFIFHEPSDLAEENVHTCGVLMDGHTGMVGASLDILVCPRDIHG
  YLAPVPKTPLAFYEVKCRAKYAFDPMDPSDPTASAYEDLMAHRSPEAFRAFIRSIPKPSVRYFAPGRVPG
  PEEALVTQDQAWSEAHASGEKRRCSAADRALVELNSGVVSEVLLFGAPDLGRHTISPVSWSSGDLVRREP
  VFANPRHPNFKQILVQGYVLDSHFPDCPPHPHLVTFIGRHRTSAEEGVTFRLEDGAGALGAAGPSKASIL
  PNQAVPIALIITPVRIDPEIYKAIQRSSRLAFDDTLAELWASRSPGPGPAAAETTSSSPTTGRSSR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001806
• ORF Size: 1878 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001806.1, NP_044613
• RefSeq ORF: 1878
• Locus ID: 2703382
• MW: 67.5 kDa