UL52 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100841

Myc-DDK-tagged ORF clone of viral ORF for helicase-primase primase subunit [Human herpesvirus 1], codon optimized for human cell expression, NP_044655

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL52
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100841 represents NCBI reference of NP_044655 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCAGGAGGATGGAAACCGGGGTGAAAGAAGAGCTGCCGGTACACCAGTAGAGGTTACCGCCTTGT
  ACGCAACTGACGGGTGTGTTATAACCTCTAGCATCGCCCTGCTCACCAATTCACTGCTGGGAGCTGAACC
  CGTGTATATCTTCAGCTACGACGCTTATACCCATGACGGCAGGGCAGACGGGCCGACCGAACAGGATCGC
  TTTGAGGAAAGTCGAGCGCTGTATCAAGCATCCGGGGGCCTGAATGGCGACAGCTTCAGGGTGACGTTTT
  GCTTGCTGGGGACAGAGGTGGGCGGCACACATCAAGCCCGCGGTAGAACCCGCCCAATGTTTGTCTGTAG
  GTTTGAGAGGGCGGATGATGTTGCGGCGCTGCAGGACGCCCTTGCCCATGGAACCCCGCTCCAGCCCGAT
  CACATCGCAGCTACCCTCGACGCCGAAGCAACATTTGCGCTGCATGCAAATATGATTCTGGCCCTGACTG
  TCGCAATCAACAATGCATCTCCACGCACAGGGAGGGACGCAGCCGCTGCCCAATATGATCAAGGCGCCTC
  TCTGAGGTCCCTGGTAGGACGGACTAGTCTGGGTCAGCGGGGCTTGACAACCTTGTACGTTCACCACGAA
  GTGAGGGTACTGGCTGCCTACCGCAGAGCATACTATGGATCTGCCCAGAGCCCCTTTTGGTTTCTCAGCA
  AATTCGGCCCCGATGAGAAATCCTTGGTGTTGACAACGAGATATTACCTGCTTCAGGCTCAAAGACTTGG
  AGGGGCGGGGGCTACGTACGACCTGCAAGCCATCAAAGATATCTGCGCTACCTATGCTATTCCCCATGCG
  CCCCGCCCTGACACTGTCTCCGCGGCAAGTCTCACTTCCTTCGCGGCTATCACGCGGTTTTGTTGCACCT
  CTCAGTACGCTCGGGGCGCTGCCGCGGCCGGATTTCCTCTCTATGTCGAGAGGAGAATAGCCGCTGATGT
  TCGGGAGACATCAGCTCTGGAAAAGTTTATCACTCACGACAGGAGCTGTCTTAGAGTCTCCGATAGGGAA
  TTCATTACATATATCTACCTGGCACACTTTGAGTGTTTCTCCCCACCTAGACTGGCAACACATCTCCGGG
  CCGTAACCACCCATGACCCCAATCCGGCCGCCTCCACAGAACAGCCCTCACCTCTGGGCAGGGAGGCCGT
  TGAGCAGTTTTTTTGCCATGTGCGCGCACAGCTGAATATTGGTGAGTACGTGAAACATAACGTTACACCC
  CGAGAAACCGTACTGGACGGAGATACAGCAAAGGCTTACCTGAGGGCTAGAACTTATGCACCCGGGGCGC
  TCACCCCTGCTCCAGCTTACTGCGGGGCAGTCGACTCAGCTACTAAGATGATGGGAAGACTGGCCGATGC
  TGAGAAACTTCTCGTGCCAAGGGGTTGGCCGGCCTTTGCTCCAGCAAGCCCGGGCGAGGATACCGCTGGT
  GGCACCCCTCCCCCCCAGACTTGTGGAATCGTTAAAAGGCTCCTGAGGTTGGCAGCCACTGAACAGCAGG
  GACCCACTCCCCCAGCAATTGCCGCGCTGATCCGAAACGCAGCAGTGCAAACGCCACTCCCGGTTTATCG
  CATCAGCATGGTCCCAACAGGCCAGGCGTTCGCCGCCCTGGCTTGGGATGACTGGGCTAGGATCACAAGA
  GACGCCAGGCTGGCCGAGGCCGTGGTTAGCGCTGAGGCAGCAGCTCACCCAGATCATGGCGCACTGGGCC
  GGCGGCTCACAGATCGAATAAGGGCTCAGGGTCCGGTGATGCCTCCAGGAGGCCTGGATGCCGGAGGTCA
  AATGTATGTGAACAGAAACGAAATATTTAACGGGGCGCTGGCAATTACAAACATTATTCTGGATCTGGAC
  ATCGCTCTGAAGGAGCCCGTGCCCTTTAGAAGGCTGCACGAGGCGCTGGGGCACTTTAGAAGGGGTGCTC
  TCGCCGCAGTGCAGCTGCTCTTCCCAGCAGCTCGCGTCGACCCCGACGCTTACCCCTGTTACTTCTTCAA
  ATCCGCTTGCCGACCCGGACCAGCGTCAGTGGGATCCGGTTCCGGCCTCGGAAATGACGACGATGGTGAC
  TGGTTTCCTTGCTACGACGATGCCGGCGATGAGGAATGGGCTGAAGATCCAGGCGCCATGGACACCAGTC
  ACGATCCTCCTGATGACGAGGTCGCCTACTTTGATTTGTGCCACGAAGTCGGCCCAACTGCCGAACCAAG
  AGAAACCGACAGCCCTGTATGCTCCTGTACCGACAAGATTGGGCTGCGGGTTTGCATGCCTGTCCCTGCC
  CCTTACGTGGTGCATGGTAGTTTGACAATGCGCGGCGTAGCTCGCGTGATACAGCAAGCTGTCCTTCTTG
  ACCGAGATTTCGTCGAAGCTATCGGGAGCTATGTCAAAAACTTTCTGTTGATTGACACAGGCGTGTATGC
  ACATGGTCACAGCCTTCGGTTGCCCTACTTTGCAAAAATTGCCCCAGATGGGCCAGCGTGTGGTAGACTT
  TTGCCCGTCTTTGTGATTCCACCCGCCTGTAAGGACGTTCCCGCTTTTGTAGCGGCTCATGCAGACCCCA
  GGCGGTTCCACTTCCACGCCCCCCCTACCTATTTGGCCTCCCCTAGAGAAATTCGGGTGCTGCATTCACT
  TGGAGGAGACTATGTGTCCTTTTTTGAACGAAAAGCATCCAGAAACGCTCTGGAACACTTCGGCCGGAGA
  GAGACACTTACCGAGGTGCTTGGTAGATATAACGTCCAACCTGATGCTGGCGGCACAGTAGAGGGTTTTG
  CTAGTGAACTTCTCGGACGAATAGTGGCTTGCATCGAGACGCATTTCCCCGAGCACGCTGGGGAGTACCA
  GGCTGTCTCTGTGAGGCGGGCCGTGTCCAAAGACGACTGGGTCCTGCTCCAGCTGGTCCCCGTGAGAGGA
  ACTTTGCAACAAAGCCTGTCTTGTCTGCGCTTCAAGCACGGCCGCGCTTCCCGAGCAACAGCTCGGACTT
  TTGTAGCCTTGAGTGTAGGAGCGAATAATAGACTGTGTGTGTCACTGTGCCAGCAGTGCTTTGCCGCGAA
  ATGCGATAGTAACCGCCTTCATACCCTCTTCACCATAGACGCGGGAACCCCTTGCTCACCGAGCGTGCCT
  TGTAGCACTAGTCAACCGTCATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100841 representing NP_044655
  Red=Cloning sites Green=Tags
  
  MGQEDGNRGERRAAGTPVEVTALYATDGCVITSSIALLTNSLLGAEPVYIFSYDAYTHDGRADGPTEQDR
  FEESRALYQASGGLNGDSFRVTFCLLGTEVGGTHQARGRTRPMFVCRFERADDVAALQDALAHGTPLQPD
  HIAATLDAEATFALHANMILALTVAINNASPRTGRDAAAAQYDQGASLRSLVGRTSLGQRGLTTLYVHHE
  VRVLAAYRRAYYGSAQSPFWFLSKFGPDEKSLVLTTRYYLLQAQRLGGAGATYDLQAIKDICATYAIPHA
  PRPDTVSAASLTSFAAITRFCCTSQYARGAAAAGFPLYVERRIAADVRETSALEKFITHDRSCLRVSDRE
  FITYIYLAHFECFSPPRLATHLRAVTTHDPNPAASTEQPSPLGREAVEQFFCHVRAQLNIGEYVKHNVTP
  RETVLDGDTAKAYLRARTYAPGALTPAPAYCGAVDSATKMMGRLADAEKLLVPRGWPAFAPASPGEDTAG
  GTPPPQTCGIVKRLLRLAATEQQGPTPPAIAALIRNAAVQTPLPVYRISMVPTGQAFAALAWDDWARITR
  DARLAEAVVSAEAAAHPDHGALGRRLTDRIRAQGPVMPPGGLDAGGQMYVNRNEIFNGALAITNIILDLD
  IALKEPVPFRRLHEALGHFRRGALAAVQLLFPAARVDPDAYPCYFFKSACRPGPASVGSGSGLGNDDDGD
  WFPCYDDAGDEEWAEDPGAMDTSHDPPDDEVAYFDLCHEVGPTAEPRETDSPVCSCTDKIGLRVCMPVPA
  PYVVHGSLTMRGVARVIQQAVLLDRDFVEAIGSYVKNFLLIDTGVYAHGHSLRLPYFAKIAPDGPACGRL
  LPVFVIPPACKDVPAFVAAHADPRRFHFHAPPTYLASPREIRVLHSLGGDYVSFFERKASRNALEHFGRR
  ETLTEVLGRYNVQPDAGGTVEGFASELLGRIVACIETHFPEHAGEYQAVSVRRAVSKDDWVLLQLVPVRG
  TLQQSLSCLRFKHGRASRATARTFVALSVGANNRLCVSLCQQCFAAKCDSNRLHTLFTIDAGTPCSPSVP
  CSTSQPSS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001806
• ORF Size: 3174 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001806.1, NP_044655
• RefSeq ORF: 3174
• Locus ID: 2703423
• MW: 114.4 kDa