UL30 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100818

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase catalytic subunit [Human herpesvirus 1], codon optimized for human cell expression, NP_044632

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100818 represents NCBI reference of NP_044632 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAGCGGAGGTGGGGGACCTCTGTCTCCTGGAGGAAAAAGCGCGGCACGAGCCGCATCTGGCTTTT
  TCGCACCTGCGGGTCCACGCGGTGCGTCACGCGGCCCACCACCCTGTCTTCGCCAGAACTTCTATAACCC
  CTACCTGGCACCTGTCGGCACTCAACAGAAGCCTACTGGTCCCACCCAAAGGCACACCTACTACTCTGAG
  TGCGACGAGTTCCGATTCATTGCCCCGCGAGTGTTGGATGAGGATGCCCCCCCAGAGAAGCGGGCCGGGG
  TGCATGATGGACATCTGAAGAGAGCACCGAAGGTTTACTGTGGAGGGGATGAAAGGGATGTGCTTCGGGT
  GGGCTCAGGTGGATTTTGGCCCCGAAGAAGTAGACTCTGGGGTGGGGTGGATCATGCTCCTGCCGGTTTC
  AATCCCACTGTCACTGTGTTCCACGTGTATGACATTCTTGAAAACGTCGAACACGCTTACGGTATGCGAG
  CCGCTCAGTTTCACGCGAGATTTATGGACGCCATCACACCAACTGGCACCGTGATCACTCTCCTGGGCCT
  TACTCCAGAAGGCCACCGCGTTGCTGTGCATGTGTACGGCACCAGGCAGTACTTCTATATGAACAAAGAA
  GAGGTGGATAGACACCTTCAGTGTAGAGCACCTCGCGACCTGTGTGAAAGAATGGCAGCAGCTCTCCGAG
  AATCACCAGGCGCTAGCTTTCGGGGTATCAGCGCAGACCATTTTGAGGCCGAAGTGGTAGAGCGCACCGA
  CGTCTACTACTATGAAACTCGACCCGCCCTGTTCTACCGCGTATATGTGCGCTCTGGAAGAGTTCTCTCT
  TACCTCTGCGATAACTTTTGTCCTGCCATTAAGAAGTATGAAGGGGGTGTCGACGCTACCACACGCTTCA
  TCTTGGATAACCCGGGGTTCGTTACATTCGGTTGGTATCGACTGAAGCCAGGTCGGAACAATACCCTCGC
  ACAACCAGCGGCCCCGATGGCCTTTGGGACATCCTCCGACGTGGAGTTCAACTGCACTGCTGATAACCTG
  GCTATAGAAGGCGGCATGAGCGACTTGCCAGCATACAAGTTGATGTGCTTTGATATCGAGTGCAAAGCAG
  GGGGAGAGGACGAACTGGCCTTCCCTGTCGCGGGGCATCCTGAAGATCTGGTGATCCAAATTTCATGCCT
  GCTGTATGATCTCTCCACCACTGCCCTTGAGCATGTGCTTCTCTTTTCTTTGGGCAGTTGCGACCTGCCC
  GAGTCTCACCTGAACGAGCTGGCCGCGCGCGGCCTGCCCACCCCAGTGGTCCTGGAATTCGACAGTGAGT
  TTGAGATGCTCCTGGCTTTTATGACCCTCGTGAAGCAGTATGGGCCTGAGTTTGTAACAGGATATAACAT
  AATTAATTTTGACTGGCCCTTTTTGCTCGCAAAACTGACCGACATCTACAAAGTACCGCTCGACGGCTAT
  GGGCGCATGAATGGCCGCGGCGTCTTCCGCGTGTGGGACATTGGCCAATCCCATTTCCAGAAAAGATCTA
  AGATTAAGGTTAACGGAATGGTGAATATCGATATGTACGGCATAATCACAGACAAGATCAAGTTGAGCAG
  TTATAAACTTAACGCAGTGGCTGAAGCCGTTCTGAAGGATAAAAAGAAGGATCTGAGTTACAGGGATATC
  CCGGCTTACTATGCCGCTGGCCCTGCTCAGCGCGGCGTCATTGGGGAGTACTGTATCCAGGACAGTCTTC
  TGGTTGGACAGCTGTTTTTCAAATTTTTGCCCCATCTGGAACTGTCCGCCGTGGCACGACTGGCCGGAAT
  CAACATAACCCGAACCATTTATGATGGGCAGCAGATCCGGGTATTCACATGTCTGTTGAGACTGGCTGAC
  CAGAAAGGTTTCATCCTTCCCGATACTCAGGGAAGATTCAGAGGCGCCGGGGGCGAGGCCCCTAAGCGAC
  CTGCGGCAGCAAGAGAGGATGAAGAACGGCCAGAAGAAGAGGGAGAGGATGAAGACGAAAGGGAAGAGGG
  CGGCGGTGAAAGAGAACCTGAAGGTGCCAGAGAGACGGCCGGGCGACATGTTGGGTATCAGGGCGCTAGG
  GTCCTCGATCCCACATCCGGATTCCATGTTAATCCTGTGGTGGTCTTCGACTTTGCATCTCTCTACCCAT
  CAATAATCCAGGCGCACAACCTTTGCTTCTCTACACTCTCCCTGAGGGCAGACGCCGTGGCTCACCTGGA
  AGCCGGGAAAGACTACCTCGAAATTGAGGTGGGGGGTCGCCGACTGTTCTTTGTGAAAGCTCACGTGCGC
  GAAAGCCTCCTTAGTATCTTGCTGAGAGATTGGCTCGCTATGAGAAAACAAATCCGGAGCAGGATTCCCC
  AGAGTAGCCCAGAAGAAGCCGTTCTTCTTGACAAACAGCAGGCTGCCATTAAGGTTGTGTGTAACTCTGT
  GTATGGCTTTACTGGGGTCCAACACGGGCTGCTCCCCTGCTTGCACGTGGCTGCTACTGTGACAACGATT
  GGACGCGAGATGCTGCTTGCCACACGCGAATACGTACATGCTCGGTGGGCAGCGTTCGAGCAGCTCCTGG
  CCGACTTTCCAGAGGCAGCAGATATGAGAGCACCCGGGCCGTACTCTATGAGAATTATCTACGGTGACAC
  AGACAGCATTTTCGTGCTTTGCCGGGGACTCACGGCCGCAGGCTTGACCGCTGTTGGGGATAAGATGGCC
  TCACACATAAGTCGCGCTCTGTTCCTGCCCCCAATCAAGCTGGAGTGCGAAAAAACCTTTACCAAACTGT
  TGCTTATAGCCAAGAAGAAGTACATTGGGGTTATCTATGGCGGGAAGATGCTTATCAAAGGAGTCGACCT
  CGTGCGAAAGAATAACTGCGCCTTCATCAACAGAACCTCCAGGGCACTTGTTGATCTCTTGTTCTATGAC
  GACACCGTTAGCGGAGCAGCTGCAGCCCTCGCTGAGCGGCCTGCGGAGGAATGGTTGGCCAGACCACTGC
  CCGAAGGACTCCAGGCATTCGGTGCCGTGCTGGTCGATGCTCACAGGCGAATTACTGATCCAGAGCGCGA
  CATTCAGGACTTCGTACTGACCGCCGAACTCTCACGGCATCCCAGAGCGTACACCAACAAACGCCTCGCT
  CATTTGACAGTGTATTACAAGCTTATGGCTCGGAGAGCTCAAGTCCCATCCATCAAAGATAGAATTCCTT
  ATGTCATTGTCGCGCAGACTCGAGAGGTTGAGGAAACCGTGGCAAGACTGGCCGCCCTGAGAGAACTGGA
  TGCCGCTGCACCTGGTGATGAGCCTGCCCCCCCTGCAGCCCTGCCATCACCAGCTAAAAGGCCCAGAGAG
  ACCCCGAGTCCAGCTGATCCACCAGGTGGGGCCAGTAAACCTCGAAAACTCCTGGTAAGCGAGCTGGCAG
  AGGATCCAGCATATGCTATTGCCCACGGTGTTGCACTTAACACCGACTATTACTTTAGCCATCTCCTGGG
  GGCTGCTTGTGTTACATTCAAAGCACTGTTCGGGAACAACGCCAAGATCACCGAGTCCCTCTTGAAGAGA
  TTTATACCAGAGGTCTGGCATCCCCCCGATGATGTCGCCGCTCGACTGCGCACAGCAGGGTTCGGCGCCG
  TCGGAGCAGGAGCAACCGCGGAGGAAACTCGACGAATGCTTCATAGGGCCTTTGATACGTTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100818 representing NP_044632
  Red=Cloning sites Green=Tags
  
  MFSGGGGPLSPGGKSAARAASGFFAPAGPRGASRGPPPCLRQNFYNPYLAPVGTQQKPTGPTQRHTYYSE
  CDEFRFIAPRVLDEDAPPEKRAGVHDGHLKRAPKVYCGGDERDVLRVGSGGFWPRRSRLWGGVDHAPAGF
  NPTVTVFHVYDILENVEHAYGMRAAQFHARFMDAITPTGTVITLLGLTPEGHRVAVHVYGTRQYFYMNKE
  EVDRHLQCRAPRDLCERMAAALRESPGASFRGISADHFEAEVVERTDVYYYETRPALFYRVYVRSGRVLS
  YLCDNFCPAIKKYEGGVDATTRFILDNPGFVTFGWYRLKPGRNNTLAQPAAPMAFGTSSDVEFNCTADNL
  AIEGGMSDLPAYKLMCFDIECKAGGEDELAFPVAGHPEDLVIQISCLLYDLSTTALEHVLLFSLGSCDLP
  ESHLNELAARGLPTPVVLEFDSEFEMLLAFMTLVKQYGPEFVTGYNIINFDWPFLLAKLTDIYKVPLDGY
  GRMNGRGVFRVWDIGQSHFQKRSKIKVNGMVNIDMYGIITDKIKLSSYKLNAVAEAVLKDKKKDLSYRDI
  PAYYAAGPAQRGVIGEYCIQDSLLVGQLFFKFLPHLELSAVARLAGINITRTIYDGQQIRVFTCLLRLAD
  QKGFILPDTQGRFRGAGGEAPKRPAAAREDEERPEEEGEDEDEREEGGGEREPEGARETAGRHVGYQGAR
  VLDPTSGFHVNPVVVFDFASLYPSIIQAHNLCFSTLSLRADAVAHLEAGKDYLEIEVGGRRLFFVKAHVR
  ESLLSILLRDWLAMRKQIRSRIPQSSPEEAVLLDKQQAAIKVVCNSVYGFTGVQHGLLPCLHVAATVTTI
  GREMLLATREYVHARWAAFEQLLADFPEAADMRAPGPYSMRIIYGDTDSIFVLCRGLTAAGLTAVGDKMA
  SHISRALFLPPIKLECEKTFTKLLLIAKKKYIGVIYGGKMLIKGVDLVRKNNCAFINRTSRALVDLLFYD
  DTVSGAAAALAERPAEEWLARPLPEGLQAFGAVLVDAHRRITDPERDIQDFVLTAELSRHPRAYTNKRLA
  HLTVYYKLMARRAQVPSIKDRIPYVIVAQTREVEETVARLAALRELDAAAPGDEPAPPAALPSPAKRPRE
  TPSPADPPGGASKPRKLLVSELAEDPAYAIAHGVALNTDYYFSHLLGAACVTFKALFGNNAKITESLLKR
  FIPEVWHPPDDVAARLRTAGFGAVGAGATAEETRRMLHRAFDTLA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001806
• ORF Size: 3705 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001806.1, NP_044632
• RefSeq ORF: 3705
• Locus ID: 2703462
• MW: 136.4 kDa