UL25 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100812

Myc-DDK-tagged ORF clone of viral ORF for DNA packaging tegument protein UL25 [Human herpesvirus 1], codon optimized for human cell expression, NP_044626

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL25
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100812 represents NCBI reference of NP_044626 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCGTATTGTCCTTTTGATGCATTGGATGTATGGGAGCACAGGCGGTTCATAGTGGCTGACAGCC
  GCAATTTCATCACACCAGAGTTTCCCAGGGACTTTTGGATGAGCCCCGTATTTAATCTGCCTCGAGAAAC
  CGCTGCCGAGCAGGTGGTTGTCCTGCAGGCCCAAAGGACCGCCGCCGCAGCCGCCCTGGAGAACGCTGCC
  ATGCAGGCCGCAGAACTCCCTGTGGACATTGAGAGAAGACTCAGGCCCATAGAGCGGAATGTTCATGAAA
  TTGCCGGGGCTCTGGAGGCTCTCGAAACAGCTGCTGCTGCTGCAGAAGAAGCTGATGCAGCGCGCGGAGA
  TGAGCCAGCTGGCGGAGGGGATGGGGGTGCTCCCCCTGGTCTGGCAGTCGCAGAGATGGAGGTCCAAATC
  GTGAGAAACGACCCTCCCCTCAGGTATGACACAAACCTGCCCGTCGACCTCCTGCACATGGTATACGCAG
  GACGAGGCGCGACTGGGTCATCCGGCGTTGTGTTCGGAACATGGTATCGGACTATTCAGGACCGGACTAT
  CACCGACTTTCCTCTGACGACAAGGTCCGCTGACTTCCGCGATGGGAGGATGTCTAAAACATTCATGACC
  GCACTGGTCTTGAGCCTGCAGGCCTGCGGGAGACTGTACGTGGGACAGCGCCACTACAGTGCCTTTGAAT
  GCGCTGTTCTTTGCCTCTATCTGCTGTACCGAAACACCCATGGGGCCGCCGACGACAGCGATAGAGCCCC
  TGTCACCTTCGGCGATCTCCTTGGGAGACTGCCAAGGTACTTGGCCTGCCTCGCAGCGGTTATTGGGACA
  GAGGGGGGCCGACCGCAGTACAGGTACCGGGATGACAAACTGCCCAAGACCCAGTTTGCCGCCGGCGGAG
  GTAGATATGAACACGGCGCACTGGCATCTCATATCGTTATAGCAACCCTTATGCACCACGGCGTTCTGCC
  AGCAGCACCAGGCGACGTCCCAAGGGACGCCTCTACACACGTGAATCCCGATGGAGTGGCCCATCACGAT
  GATATTAATCGGGCCGCAGCAGCCTTTCTCTCCAGGGGGCACAATCTTTTCTTGTGGGAGGATCAGACAT
  TGCTGCGGGCCACGGCTAATACTATCACTGCCCTGGGAGTAATCCAGCGACTCCTCGCTAATGGTAACGT
  ATACGCTGATAGACTGAACAATAGGCTGCAGTTGGGTATGTTGATCCCAGGAGCTGTGCCATCAGAGGCT
  ATCGCGCGGGGGGCGTCAGGGTCAGATTCCGGCGCCATCAAGTCAGGAGACAATAATCTGGAAGCTCTGT
  GTGCGAACTACGTGCTGCCCCTCTACCGAGCTGATCCTGCCGTGGAGCTGACCCAGCTTTTTCCTGGTCT
  CGCTGCATTGTGCCTCGACGCGCAGGCTGGCAGACCCGTGGGGTCTACTCGGCGAGTTGTGGACATGTCA
  TCTGGGGCAAGGCAGGCCGCCCTGGTGAGACTCACCGCATTGGAGCTGATCAACCGCACTCGCACAAACC
  CAACCCCAGTGGGAGAGGTGATCCACGCCCACGACGCCCTTGCTATCCAGTACGAGCAGGGTCTCGGGCT
  GCTGGCCCAGCAGGCCCGCATTGGACTGGGCAGCAATACCAAGCGCTTTTCTGCCTTCAACGTAAGCAGT
  GATTACGACATGCTCTACTTTTTGTGTCTGGGATTCATCCCTCAATATCTTTCTGCTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100812 representing NP_044626
  Red=Cloning sites Green=Tags
  
  MDPYCPFDALDVWEHRRFIVADSRNFITPEFPRDFWMSPVFNLPRETAAEQVVVLQAQRTAAAAALENAA
  MQAAELPVDIERRLRPIERNVHEIAGALEALETAAAAAEEADAARGDEPAGGGDGGAPPGLAVAEMEVQI
  VRNDPPLRYDTNLPVDLLHMVYAGRGATGSSGVVFGTWYRTIQDRTITDFPLTTRSADFRDGRMSKTFMT
  ALVLSLQACGRLYVGQRHYSAFECAVLCLYLLYRNTHGAADDSDRAPVTFGDLLGRLPRYLACLAAVIGT
  EGGRPQYRYRDDKLPKTQFAAGGGRYEHGALASHIVIATLMHHGVLPAAPGDVPRDASTHVNPDGVAHHD
  DINRAAAAFLSRGHNLFLWEDQTLLRATANTITALGVIQRLLANGNVYADRLNNRLQLGMLIPGAVPSEA
  IARGASGSDSGAIKSGDNNLEALCANYVLPLYRADPAVELTQLFPGLAALCLDAQAGRPVGSTRRVVDMS
  SGARQAALVRLTALELINRTRTNPTPVGEVIHAHDALAIQYEQGLGLLAQQARIGLGSNTKRFSAFNVSS
  DYDMLYFLCLGFIPQYLSAV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001806
• ORF Size: 1740 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001806.1, NP_044626
• RefSeq ORF: 1740
• Locus ID: 2703377
• MW: 62.7 kDa