US3 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100851

Myc-DDK-tagged ORF clone of viral ORF for serine/threonine protein kinase US3 [Human herpesvirus 1], codon optimized for human cell expression, NP_044665

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100851 represents NCBI reference of NP_044665 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGCCGGAAGTTTTGTCGGGTGTACGGGGGCCAAGGGCGGCGAAAGGAAGAAGCCGTCCCTCCCG
  AAACTAAGCCCTCTAGGGTGTTCCCCCATGGTCCTTTTTATACTCCAGCTGAGGATGCCTGTTTGGATTC
  CCCACCACCCGAAACCCCTAAGCCTAGCCACACCACCCCTCCTAGTGAAGCTGAGCGCTTGTGCCACCTC
  CAGGAGATACTGGCTCAGATGTACGGCAACCAGGACTATCCCATCGAGGACGACCCTTCCGCTGACGCTG
  CCGATGATGTGGATGAGGACGCCCCTGACGATGTTGCGTACCCCGAAGAATATGCGGAGGAACTCTTTCT
  CCCCGGTGATGCTACTGGTCCGCTCATTGGCGCTAATGACCATATTCCCCCTCCCTGTGGGGCTTCACCG
  CCTGGCATTAGGCGGAGATCACGGGACGAGATTGGCGCTACCGGATTTACTGCCGAAGAGCTTGACGCCA
  TGGATCGAGAGGCCGCTCGGGCGATTTCCAGAGGTGGGAAGCCTCCATCTACCATGGCAAAACTGGTCAC
  AGGCATGGGATTTACCATCCATGGTGCTCTTACTCCTGGATCCGAAGGTTGCGTTTTCGACAGCTCACAT
  CCAGACTATCCTCAAAGGGTTATAGTCAAAGCAGGCTGGTATACGTCTACCAGCCACGAGGCCAGACTGC
  TGCGCCGGCTCGACCACCCAGCCATACTCCCTCTCCTTGACCTCCACGTTGTTTCCGGAGTGACTTGCCT
  CGTTCTCCCAAAATATCAGGCAGATTTGTACACGTACCTGTCTAGGCGGCTCAATCCACTGGGACGGCCC
  CAGATCGCAGCCGTGAGCAGACAGTTGCTCTCAGCGGTTGATTACATTCATCGGCAAGGCATTATTCACC
  GAGACATAAAAACCGAGAATATTTTCATCAACACGCCGGAAGATATCTGTCTGGGCGACTTCGGAGCTGC
  CTGTTTCGTGCAGGGTTCCCGCTCCAGCCCTTTCCCATACGGCATCGCTGGAACCATCGATACAAACGCC
  CCCGAGGTGTTGGCAGGAGATCCGTACACCACCACCGTGGATATTTGGAGCGCCGGCCTGGTGATATTTG
  AGACAGCCGTACACAACGCCTCATTGTTCTCCGCCCCTCGCGGCCCCAAAAGAGGACCTTGTGATTCCCA
  GATTACCAGAATCATCCGACAAGCGCAGGTTCACGTGGACGAGTTCAGTCCACATCCTGAATCTCGCCTT
  ACCAGTCGCTACAGGAGCCGGGCTGCTGGCAACAACCGCCCCCCATACACTAGGCCTGCTTGGACCAGAT
  ACTATAAGATGGATATTGATGTGGAGTATCTCGTGTGCAAGGCACTTACATTCGATGGGGCCTTGCGCCC
  ATCTGCAGCCGAGCTGTTGTGCCTGCCACTCTTTCAACAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100851 representing NP_044665
  Red=Cloning sites Green=Tags
  
  MACRKFCRVYGGQGRRKEEAVPPETKPSRVFPHGPFYTPAEDACLDSPPPETPKPSHTTPPSEAERLCHL
  QEILAQMYGNQDYPIEDDPSADAADDVDEDAPDDVAYPEEYAEELFLPGDATGPLIGANDHIPPPCGASP
  PGIRRRSRDEIGATGFTAEELDAMDREAARAISRGGKPPSTMAKLVTGMGFTIHGALTPGSEGCVFDSSH
  PDYPQRVIVKAGWYTSTSHEARLLRRLDHPAILPLLDLHVVSGVTCLVLPKYQADLYTYLSRRLNPLGRP
  QIAAVSRQLLSAVDYIHRQGIIHRDIKTENIFINTPEDICLGDFGAACFVQGSRSSPFPYGIAGTIDTNA
  PEVLAGDPYTTTVDIWSAGLVIFETAVHNASLFSAPRGPKRGPCDSQITRIIRQAQVHVDEFSPHPESRL
  TSRYRSRAAGNNRPPYTRPAWTRYYKMDIDVEYLVCKALTFDGALRPSAAELLCLPLFQQK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001806
• ORF Size: 1443 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001806.1, NP_044665
• RefSeq ORF: 1443
• Locus ID: 2703401
• MW: 52.8 kDa