US5 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100853

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein J [Human herpesvirus 1], codon optimized for human cell expression, NP_044667

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100853 represents NCBI reference of NP_044667 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCTGAGAGCTGTGTGGCACCTGGGCCTTCTTGGAAGCCTCGTTGGAGCAGTGCTGGCAGCCACCC
  ATAGAGGACCAGCCGCGAACACCACTGATCCCCTCACACATGCACCAGTTAGCCCCCATCCTTCACCGCT
  GGGGGGCTTCGCCGTGCCACTCGTCGTTGGTGGGCTGTGTGCTGTGGTGTTGGGCGCAGCTTGCCTTCTG
  GAACTGCTTCGAAGGACATGTCGGGGGTGGGGAAGGTATCATCCCTACATGGACCCAGTAGTAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100853 representing NP_044667
  Red=Cloning sites Green=Tags
  
  MSLRAVWHLGLLGSLVGAVLAATHRGPAANTTDPLTHAPVSPHPSPLGGFAVPLVVGGLCAVVLGAACLL
  ELLRRTCRGWGRYHPYMDPVVV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001806
• ORF Size: 276 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001806.1, NP_044667
• RefSeq ORF: 276
• Locus ID: 2703406
• MW: 9.6 kDa