US8 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100856

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein E [Human herpesvirus 1], codon optimized for human cell expression, NP_044670

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100856 represents NCBI reference of NP_044670 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCGAGGTGCCGTTGTGGGATTTTTGCTGGGAGTCTGCGTTGTGTCATGCCTTGCCGGGACCCCAA
  AAACTAGCTGGCGGAGGGTGTCTGTAGGAGAGGATGTCTCACTCCTTCCAGCTCCAGGTCCTACCGGGCG
  CGGACCAACCCAGAAGCTTCTGTGGGCTGTTGAACCCCTTGACGGCTGCGGGCCTCTCCACCCCTCATGG
  GTGTCCCTGATGCCCCCTAAGCAGGTCCCCGAAACTGTCGTGGACGCGGCATGCATGCGGGCTCCTGTTC
  CCCTTGCGATGGCATATGCGCCACCTGCACCCTCAGCAACTGGCGGTTTGCGCACAGATTTTGTGTGGCA
  GGAGCGAGCTGCCGTGGTGAATCGGAGCCTGGTGATCCACGGGGTGAGAGAGACCGATAGCGGGCTGTAT
  ACGCTGAGCGTTGGTGACATCAAGGACCCCGCCAGACAGGTGGCCTCTGTGGTGCTTGTCGTCCAGCCTG
  CTCCAGTGCCTACCCCCCCTCCTACACCAGCTGATTATGACGAAGACGATAACGACGAAGGGGAGGATGA
  GAGCCTGGCAGGGACCCCCGCTAGCGGAACACCTCGACTGCCCCCTCCTCCTGCTCCTCCACGCTCCTGG
  CCATCAGCACCTGAGGTGTCCCATGTGAGGGGGGTTACTGTAAGAATGGAAACACCCGAGGCAATTCTGT
  TTAGCCCAGGCGAGACATTCAGTACTAATGTGTCTATCCACGCCATTGCCCACGACGACCAGACCTACTC
  AATGGATGTGGTTTGGCTCCGCTTTGACGTACCCACCTCTTGCGCCGAGATGAGAATCTACGAAAGTTGT
  CTGTACCACCCCCAGCTCCCTGAGTGTCTCAGTCCTGCCGATGCCCCTTGCGCTGCTAGCACCTGGACCA
  GTCGGCTGGCAGTTCGGAGTTACGCCGGCTGTTCCCGAACTAACCCTCCTCCCAGGTGTAGCGCAGAAGC
  TCACATGGAGCCCGTTCCTGGACTTGCCTGGCAGGCTGCCTCTGTCAACTTGGAATTTAGAGATGCATCT
  CCACAGCATTCTGGTTTGTACTTGTGCGTGGTATACGTGAATGATCACATCCACGCTTGGGGCCATATCA
  CTATCAGCACCGCTGCTCAGTACAGGAATGCAGTTGTCGAGCAACCTCTGCCCCAGCGCGGAGCTGATCT
  TGCCGAGCCCACTCACCCTCATGTGGGCGCACCCCCCCACGCTCCTCCTACACATGGCGCTCTGAGATTG
  GGCGCTGTTATGGGTGCCGCCCTGTTGCTCTCCGCTCTCGGCTTGAGTGTCTGGGCCTGCATGACGTGTT
  GGCGGAGAAGAGCCTGGAGAGCTGTCAAATCCCGGGCCTCCGGCAAAGGCCCTACTTACATCAGGGTGGC
  GGATAGCGAGCTGTACGCCGACTGGTCCTCTGACTCCGAAGGTGAACGAGACCAGGTGCCCTGGCTGGCT
  CCACCCGAGAGACCAGACAGTCCCAGCACCAACGGGTCTGGATTCGAGATCTTGAGCCCCACCGCACCTA
  GTGTATATCCGCGAAGCGATGGTCACCAGTCCAGGCGACAACTGACCACGTTCGGCTCCGGCCGACCCGA
  CAGGCGCTACAGCCAAGCCTCCGATTCAAGCGTGTTCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100856 representing NP_044670
  Red=Cloning sites Green=Tags
  
  MDRGAVVGFLLGVCVVSCLAGTPKTSWRRVSVGEDVSLLPAPGPTGRGPTQKLLWAVEPLDGCGPLHPSW
  VSLMPPKQVPETVVDAACMRAPVPLAMAYAPPAPSATGGLRTDFVWQERAAVVNRSLVIHGVRETDSGLY
  TLSVGDIKDPARQVASVVLVVQPAPVPTPPPTPADYDEDDNDEGEDESLAGTPASGTPRLPPPPAPPRSW
  PSAPEVSHVRGVTVRMETPEAILFSPGETFSTNVSIHAIAHDDQTYSMDVVWLRFDVPTSCAEMRIYESC
  LYHPQLPECLSPADAPCAASTWTSRLAVRSYAGCSRTNPPPRCSAEAHMEPVPGLAWQAASVNLEFRDAS
  PQHSGLYLCVVYVNDHIHAWGHITISTAAQYRNAVVEQPLPQRGADLAEPTHPHVGAPPHAPPTHGALRL
  GAVMGAALLLSALGLSVWACMTCWRRRAWRAVKSRASGKGPTYIRVADSELYADWSSDSEGERDQVPWLA
  PPERPDSPSTNGSGFEILSPTAPSVYPRSDGHQSRRQLTTFGSGRPDRRYSQASDSSVFW
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001806
• ORF Size: 1650 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001806.1, NP_044670
• RefSeq ORF: 1650
• Locus ID: 2703448
• MW: 59.1 kDa