US1 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100849

Myc-DDK-tagged ORF clone of viral ORF for regulatory protein ICP22 [Human herpesvirus 1], codon optimized for human cell expression, NP_044663

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100849 represents NCBI reference of NP_044663 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACATCTCACCGGGCGCATTTGCCCCATGCGTCAAGGCAAGAAGACCCGCCTTGAGGAGTCCAC
  CCCTTGGAACTCGCAAGAGGAAACGGCCCAGTCGGCCCCTCAGTTCCGAGAGCGAAGTCGAGAGTGATAC
  TGCTCTGGAGTCCGAAGTGGAGTCAGAAACTGCCTCCGACTCTACCGAGAGTGGAGACCAGGACGAAGCA
  CCCAGGATTGGAGGGAGACGCGCCCCTCGAAGACTGGGGGGACGGTTCTTCCTCGATATGTCAGCTGAGT
  CTACAACCGGTACCGAGACCGACGCCTCTGTCAGCGATGACCCTGATGATACTTCTGACTGGTCCTACGA
  TGACATCCCGCCACGCCCCAAGAGGGCACGGGTCAATCTGCGGCTTACCTCCTCCCCAGATCGCCGCGAC
  GGCGTGATCTTCCCTAAAATGGGGAGAGTCAGGTCCACACGGGAGACACAGCCCCGCGCCCCCACTCCAT
  CTGCTCCCTCTCCTAACGCAATGTTGAGAAGATCCGTTCGCCAGGCTCAAAGAAGGTCATCTGCAAGATG
  GACCCCGGACCTGGGATACATGAGGCAGTGCATTAACCAGCTTTTCCGCGTTCTTCGGGTCGCTAGAGAT
  CCTCACGGGAGTGCTAATAGGCTGAGGCATTTGATACGCGACTGTTACCTGATGGGGTATTGTCGCGCCA
  GACTGGCGCCAAGGACATGGTGTAGGCTCCTCCAAGTTAGCGGGGGAACTTGGGGGATGCATCTCCGCAA
  CACCATTCGGGAGGTGGAGGCACGGTTCGATGCTACAGCGGAACCGGTTTGTAAACTGCCCTGCTTGGAA
  ACTCGACGGTATGGCCCTGAGTGTGACCTCTCTAATCTGGAAATTCACCTCTCCGCCACAAGCGACGACG
  AAATCTCAGACGCTACTGACTTGGAGGCAGCTGGCAGTGATCATACACTGGCTAGTCAAAGTGATACAGA
  AGATGCACCCTCTCCAGTAACCTTGGAGACACCCGAACCGCGGGGATCTCTTGCAGTGCGGCTGGAAGAT
  GAGTTTGGTGAGTTTGATTGGACCCCACAGGAGGGGTCTCAGCCCTGGCTTAGCGCCGTTGTGGCAGATA
  CATCTTCTGTGGAGAGGCCCGGACCCTCTGACAGTGGAGCCGGACGAGCCGCGGAAGATCGCAAGTGCCT
  GGATGGCTGCCGGAAGATGCGGTTTAGTACTGCCTGCCCATATCCGTGTTCAGACACATTCCTGCGACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100849 representing NP_044663
  Red=Cloning sites Green=Tags
  
  MADISPGAFAPCVKARRPALRSPPLGTRKRKRPSRPLSSESEVESDTALESEVESETASDSTESGDQDEA
  PRIGGRRAPRRLGGRFFLDMSAESTTGTETDASVSDDPDDTSDWSYDDIPPRPKRARVNLRLTSSPDRRD
  GVIFPKMGRVRSTRETQPRAPTPSAPSPNAMLRRSVRQAQRRSSARWTPDLGYMRQCINQLFRVLRVARD
  PHGSANRLRHLIRDCYLMGYCRARLAPRTWCRLLQVSGGTWGMHLRNTIREVEARFDATAEPVCKLPCLE
  TRRYGPECDLSNLEIHLSATSDDEISDATDLEAAGSDHTLASQSDTEDAPSPVTLETPEPRGSLAVRLED
  EFGEFDWTPQEGSQPWLSAVVADTSSVERPGPSDSGAGRAAEDRKCLDGCRKMRFSTACPYPCSDTFLRP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001806
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001806.1, NP_044663
• RefSeq ORF: 1260
• Locus ID: 2703435
• MW: 46.5 kDa