ORF11 (NC_009333) Virus Tagged ORF Clone

CAT#: VC101637

Myc-DDK-tagged ORF clone of viral ORF for ORF11 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129357

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101637 represents NCBI reference of YP_001129357 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGGAAAGCGAGCAGTGCTCAAGGGCGTTTGCTGTGTGCAGTAGCAAGCGCCAGCTTGGCCGGG
  GAGAGCCCGTTTGGGACTCCGTGATCCACCCCTCTCACATTGTGATATCAAACCGAGTACCCCTGCTCCT
  TCGGGAGCAAAACATTTGCCTGCCTTCTTGTCCTAGCGTTGGGCAAATTCTGCATCGGGCCTATCCCAAT
  TTCACATTCGACAACACTCACCGGAAACAACAGACTGAGACTTATACCGCCTTTTACGCCTTTGGTGACC
  AGAACAACAAGGTCCGAATCCTGCCTACAGTTGTAGAGTCCTCAAGTAGCGTTCTCATTTTTAGGCTCCG
  GGCGTCTGTATCCGCAAACATAGCCGTGGGTGGGTTGAAGATTATTATCCTGGCTCTGACACTTGTTCAC
  GCCCAGGGCGTCTATCTGCGATGCGGCAAAGACCTGAGTACACCTCACTGTGCTCCCGCTATTGTCCAGA
  GAGAAGTGCTGTCATCTGGCTTCGAGCCGCAATTTACTGTAACAGGGATCCCAGTGACTTCTTCCAATCT
  TAATCAGTGTTATTTTCTTGTGCGGAAACCAAAGTCCAGGCTGGCCAAGCCGTTTGCACGGCTCTCCGCT
  GAAACCACCGAAGAGTGCCGGGTGCGATCAATACGGCTGGGAAAAACGCATCTGCGCATCTCCGTGACTG
  CTCCAGCACAGGAAACTCCCGTCTGGGGACTCGTGACCACTAGTTTTTCACTGACTCCTACAGCCCCCCT
  CGCCTTTGACCGCAATCCTTACAATCACGAGACATTTGCCTGTAATGCGCGCCACTACATTCCCGTGATC
  TACTCAGGACCCAAGATCACTCTGGCTCCCAGAGGTAGACAGGTGGTGTGGCACAACAACAGTTACACTA
  GCAGCCTGCCATGCAAAGTCACCGCCATCGTGTCCAACCATTGCTGTAATTGCGACATTTTTCTGGAAGA
  CTCTGAGTGGCGGCCCAACAAACCTGCCCCCCTCAAGCTCGTCAACACATCCGATCACCCCGTGATCCTG
  GAACCAGATACCCATATTGGTAATGCGTTGTTTATTATTGCACCCAAAGCTAGAGGACTCAGAAGGCTGA
  CTCGACTTACCACAAAAACCATTGAGCTGCCGGGTGGTGTTAAGATTGACTCCAGGAAGCTGCAGACTTT
  CCGGAAGATGTATGTGGCTACAGGACGCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101637 representing YP_001129357
  Red=Cloning sites Green=Tags
  
  MAQESEQCSRAFAVCSSKRQLGRGEPVWDSVIHPSHIVISNRVPLLLREQNICLPSCPSVGQILHRAYPN
  FTFDNTHRKQQTETYTAFYAFGDQNNKVRILPTVVESSSSVLIFRLRASVSANIAVGGLKIIILALTLVH
  AQGVYLRCGKDLSTPHCAPAIVQREVLSSGFEPQFTVTGIPVTSSNLNQCYFLVRKPKSRLAKPFARLSA
  ETTEECRVRSIRLGKTHLRISVTAPAQETPVWGLVTTSFSLTPTAPLAFDRNPYNHETFACNARHYIPVI
  YSGPKITLAPRGRQVVWHNNSYTSSLPCKVTAIVSNHCCNCDIFLEDSEWRPNKPAPLKLVNTSDHPVIL
  EPDTHIGNALFIIAPKARGLRRLTRLTTKTIELPGGVKIDSRKLQTFRKMYVATGRS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009333
• ORF Size: 1221 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009333.1, YP_001129357
• RefSeq ORF: 1221
• Locus ID: 4961439
• MW: 45.3 kDa