ORF49 (NC_009333) Virus Tagged ORF Clone

CAT#: VC101682

Myc-DDK-tagged ORF clone of viral ORF for ORF49 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129402

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF49
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101682 represents NCBI reference of YP_001129402 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTCACGACGCCCCCTCAAGGATCACCTGTTTAACCACCTGTTTCGGTATCACTACCCCTCCTGGG
  ACCAGATCTTGCAGGAACTCGACACATTGAGTGTCGCCACACTTAATCCTGATTGTCACGTACCGGCTCT
  GAACGTGGAGAAAACCCTGTATCTGGCCAAGACGATCCAAATATTGGTGCAGCACCGACAGTCCGAGCCA
  TATCTGGTCCCCGCCGCTAGGGCCAATCTGGCCTACTCACTGCAGCAACTGTATAAATTGGGGAACGACA
  AGATTCGCGGTGTTATCAACGGGATGCTGCCCCTGGTCGACGCTGGTTGCATTGGCTTCGAACGCGAGCT
  CATCAAAGGACTTCCCCGCGTGCTGACCCTGCAGTATCCGCACACTGCACCCCTTGAATCAGAGCCGCCG
  ACGGCCGATTGCACAGAATGGTGCTTGTCACACTTCGTAGGGGCATCAGGCAGGCTGCGGTCCGAAGTGC
  GGGATATTCTTACAACTCATAATGGAACATGTGCGCCATCTTTTCAGTGGATGGCAAGTGTTGTCAAAAA
  ATTCTTTCTTGTGGAGACGGTGATTTATGAGGATTTCCAGGACACCGACTTCAATGTGCAACTGAACCTG
  TGCTTCTTTTGGACAGCAGTAGTGCAAATGTACCAGCGGTGTATCTACGAACAGAAACTGGTGCACATCA
  TCTCAACATCCCTGACACTCTTGAAGTCCACAGCTAGGAGCTTCTTCGCATGGTACGATCTCTATCGACC
  CAATCTGGGTTCCGCGGCCCTGGTCAAGTACACAGAACACCTTATTAGGGCTCTGACACCCGACTGCTCC
  GACGTAGAGCTGGGAGAGCTGTGCAGTCACCTGCATCACTGCAAGCACGCACTGTTTTCTATCCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101682 representing YP_001129402
  Red=Cloning sites Green=Tags
  
  MTSRRPLKDHLFNHLFRYHYPSWDQILQELDTLSVATLNPDCHVPALNVEKTLYLAKTIQILVQHRQSEP
  YLVPAARANLAYSLQQLYKLGNDKIRGVINGMLPLVDAGCIGFERELIKGLPRVLTLQYPHTAPLESEPP
  TADCTEWCLSHFVGASGRLRSEVRDILTTHNGTCAPSFQWMASVVKKFFLVETVIYEDFQDTDFNVQLNL
  CFFWTAVVQMYQRCIYEQKLVHIISTSLTLLKSTARSFFAWYDLYRPNLGSAALVKYTEHLIRALTPDCS
  DVELGELCSHLHHCKHALFSIQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009333
• ORF Size: 906 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009333.1, YP_001129402
• RefSeq ORF: 906
• Locus ID: 4961448
• MW: 34.6 kDa