ORF43 (NC_009333) Virus Tagged ORF Clone

CAT#: VC101675

Myc-DDK-tagged ORF clone of viral ORF for ORF43 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129395

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF43
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101675 represents NCBI reference of YP_001129395 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCGCATGAACCCAGGACTGGGTAGCAGTATAAGCGTGCACCCAAGCGAGCTGAGCATCTCCCTTT
  TTGAAATCCTCCAGGGGAAATATAGCTATGTGAGGGGACAGACGCTGCACTGTTCTCTTCGCAACCCTGG
  CGTCTTCTTTCGGCAGCTGTTCATTCATCTGTACAAGAACGCCCTCGCTAACTGTAGCTACGACCACGTC
  CTGTCTGACTGGAGAACATATGAATCATCTGCCAAAACCAGGTGGCCCGAGAAGGAGGCCCAGTGGGGAA
  GCTATAGGCGCAGTACCTTTGATAGCTGGGCGCAGACCATGCGCATGACATTGGACCACTTGCTTTTGAA
  TGCGATCAACAGAGTCCTGTATGCAAAAACACAGTTGTCATACGAACGCTATGTGGATTGGGTGGTCACC
  GTAGGGATGGTCCCCGTGGTGAAACACACTCCCGATCACAAGCTTGTGAATTCAATCCAGGAGCAGCTGA
  TGAAAGATTGCCAGCGGTTGGCCTCTGGCGAAAAGACAATTGGCAGAATCTTGACTAGTGTGACCCAGGA
  GATTTCCAATTTGGTTTCCTCCTTGTCTGCTTTGTATATCCCTGGCTATAGTGAAGTCAGTATCGACTAC
  GACTGCGTGAAAAACACCTTCGTCGGACTGTACAAACAGAAGAGAGTGCACGTCGAGGTAATTACCATGC
  CAGCAATACTGGCCGGTAGAGTGATCTTCGACTCTCCAATCCAGCGCATGTATACAAGTATTATGAGTTG
  TCACCGCACAGCGGAACATGCCAAGCTTTGCCAACTCTTGAACACTGCTCCGACAAAGGCTTTGGTGGGG
  AGCGCCTGCAATAATGTTTACAAAGACATAATGACACACCTCGAACAGGCTAGTCAGAGAACCGACCCTA
  AGCGGGAGCTGCTGAACTTGCTCATGAAACTGGCTGAAAATAAAACTGTAAGCGGCGTGACCGATGTGGT
  TGAAGATTTTGTTACTGACGTGAGCCAAAATATCGTGGATAAGAATAAACTCTTCGGCACTGGCCAGGAG
  ACAACCACACAGGGACTGCGGAGGCAGGTGAGTAACTCAGTTTTTAAATGTTTGACCAATCAGATCAATG
  AACAGTTTGACACCATAACACAGCTGGAGAAGGAAAGAGAGCTTTGCATGAAACGCCTCAAATGTATCGA
  GACACAGCTGTCACACCAGCAGCCTGGTGACGCCAAAGGCCCTGGGTCTGTAAATCTGCTCACCGCCAAC
  ACGTTCCAGTCCCTCGGCCGACTGCAGGACCCCAGTTTGCAGCTGACCTCTTCACACATCCCATCCGGTT
  CCGCAGTTCTGAACAGTTTCTTTTCTTCCTATATCCCGCCCGTAAGAGAGTCCATGAAGGACCTGACTAA
  CCTCTGGGAGTCTGAGATGTTCCAAACATATAAGCTGGCCCCTGTGGTCGATAATCAGGGGCAACGGCTT
  AGCGTAACATACTCCCAGGATACCATCTCAATTTTGCTCGGTCCGTTCACTTACGTCATTGCGGACTTGT
  TGCAGATGGAGCTCATTAGCCACTCCTTTGTCAGCTCATCCCTCCAGGACATCGCCGCCTATCTTTACCA
  GACCAGTCGCCTCTTTGTTTACATTACAGACGTTGGCCAGAAATATTGCCTGGTGACCCCCCCCTTCGAA
  AACGTTCCAGGAAAGGGACCAGGGGAAACAGACTGGAGCGCTAATGAGTATTCATGCCCTGAAGACAGCA
  GGGTGAGAAGGGGTCTGTCAAGAATCCCCCCTCCATGCGGTGCCCCGCCGTGTCCAGGCTCCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101675 representing YP_001129395
  Red=Cloning sites Green=Tags
  
  MLRMNPGLGSSISVHPSELSISLFEILQGKYSYVRGQTLHCSLRNPGVFFRQLFIHLYKNALANCSYDHV
  LSDWRTYESSAKTRWPEKEAQWGSYRRSTFDSWAQTMRMTLDHLLLNAINRVLYAKTQLSYERYVDWVVT
  VGMVPVVKHTPDHKLVNSIQEQLMKDCQRLASGEKTIGRILTSVTQEISNLVSSLSALYIPGYSEVSIDY
  DCVKNTFVGLYKQKRVHVEVITMPAILAGRVIFDSPIQRMYTSIMSCHRTAEHAKLCQLLNTAPTKALVG
  SACNNVYKDIMTHLEQASQRTDPKRELLNLLMKLAENKTVSGVTDVVEDFVTDVSQNIVDKNKLFGTGQE
  TTTQGLRRQVSNSVFKCLTNQINEQFDTITQLEKERELCMKRLKCIETQLSHQQPGDAKGPGSVNLLTAN
  TFQSLGRLQDPSLQLTSSHIPSGSAVLNSFFSSYIPPVRESMKDLTNLWESEMFQTYKLAPVVDNQGQRL
  SVTYSQDTISILLGPFTYVIADLLQMELISHSFVSSSLQDIAAYLYQTSRLFVYITDVGQKYCLVTPPFE
  NVPGKGPGETDWSANEYSCPEDSRVRRGLSRIPPPCGAPPCPGSA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009333
• ORF Size: 1815 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009333.1, YP_001129395
• RefSeq ORF: 1815
• Locus ID: 4961497
• MW: 68.0 kDa