ORF69 (NC_009333) Virus Tagged ORF Clone

CAT#: VC101707

Myc-DDK-tagged ORF clone of viral ORF for ORF69 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129427

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF69
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101707 represents NCBI reference of YP_001129427 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAAATCAGTGTCCTCACATATCTCTCTGGCTACCTCTACTGGGAGATCCGGACCTCGCGACATCC
  GGCGGTGCCTGAGCAGCCGCCTGCGCTCAGTTCCTCCCGGCGCCCGAAGTGCCAGCGTTTCCTCAAAACA
  CCGCAACGGATTGCGCAAATTTATCAGTGATAAAGTTTTTTTTAGCATTCTGTCCCACCGGCATGAGCTC
  GGAGTGGATTTTCTGAGAGAAATGGAAACACCAATCTGTACGAGCAAGACTGTGATGCTTCCATTGGACC
  TGAGCACAGTGGCCCCGGGAAGATGCGTATCACTCAGCCCCTTCGGCCATAGCTCCAATATGGGCTTTCA
  GTGCGCGCTGTGCCCCAGCACCGAGAACCCTACTGTGGCTCAGGGGAGCAGACCGCAGACAATGGTGGGA
  GACGCCCTTAAGAAAAACAACGAGCTGTGTTCTGTCGCCCTGGCCTTCTATCATCATGCGGACAAAGTGA
  TACAACACAAGACCTTTTATCTGTCACTGTTGAGTCACTCCATGGATGTAGTGCGACAGTCCTTCCTGCA
  GCCTGGACTGCTCTATGCCAACCTTGTTCTCAAAACTTTTGGCCATGATCCCCTCCCTATTTTTACTACT
  AACAATGGAATGTTGACTATGTGTATCTTGTTCAAGACTCGAGCCCTCCACCTCGGAGAAACTGCTCTGA
  GACTGCTCATGGACAACCTCCCAAACTACAAGATCTCAGCCGACTGCTGTAGACAGTCTTACGTTGTGAA
  ATTCGTGCCAACACACCCTGATACTGCCAGCATTGCAGTGCAGGTGCACACGATCTGTGAAGCAGTGGCC
  GCCCTCGATTGTACAGACGAAATGAGAGACGACATCCAGAAGGGTACTGCTCTGGTGAACGCTTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101707 representing YP_001129427
  Red=Cloning sites Green=Tags
  
  MPKSVSSHISLATSTGRSGPRDIRRCLSSRLRSVPPGARSASVSSKHRNGLRKFISDKVFFSILSHRHEL
  GVDFLREMETPICTSKTVMLPLDLSTVAPGRCVSLSPFGHSSNMGFQCALCPSTENPTVAQGSRPQTMVG
  DALKKNNELCSVALAFYHHADKVIQHKTFYLSLLSHSMDVVRQSFLQPGLLYANLVLKTFGHDPLPIFTT
  NNGMLTMCILFKTRALHLGETALRLLMDNLPNYKISADCCRQSYVVKFVPTHPDTASIAVQVHTICEAVA
  ALDCTDEMRDDIQKGTALVNAL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009333
• ORF Size: 906 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009333.1, YP_001129427
• RefSeq ORF: 906
• Locus ID: 4961444
• MW: 33.2 kDa