ORF33 (NC_009333) Virus Tagged ORF Clone

CAT#: VC101666

Myc-DDK-tagged ORF clone of viral ORF for ORF33 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129386

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF33
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101666 represents NCBI reference of YP_001129386 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTCGACGCAGGAAATTGAGAAATTTCCTGAATAAGGAGTGCATATGGACCGTTAATCCCATGA
  GTGGCGACCACATCAAGGTGTTCAATGCGTGCACATCCATCTCTCCCGTCTACGATCCTGAGCTTGTGAC
  CTCTTACGCGCTGAGCGTCCCTGCGTATAATGTTTCCGTGGCTATCCTTCTCCACAAGGTGATGGGACCC
  TGCGTGGCAGTGGGTATAAATGGCGAAATGATCATGTATGTAGTGTCCCAGTGTGTTTCTGTTAGGCCTG
  TGCCCGGCAGGGACGGCATGGCTCTTATCTATTTTGGGCAGTTTCTGGAGGAGGCTTCAGGCCTTAGATT
  TCCCTACATAGCGCCCCCTCCAAGTAGGGAGCACGTGCCCGACTTGACAAGGCAGGAACTCGTCCACACC
  AGTCAGGTGGTTCGACGGGGCGACCTGACAAATTGCACCATGGGGCTGGAATTTCGGAATGTGAACCCTT
  TTGTCTGGTTGGGCGGAGGATCTGTTTGGTTGTTGTTTCTGGGCGTTGACTACATGGCCTTCTGCCCCGG
  CGTGGATGGCATGCCTTCACTGGCACGAGTTGCGGCTCTTCTGACCAGGTGCGACCACCCCGATTGTGTT
  CATTGTCACGGTCTCAGGGGCCATGTAAACGTCTTTCGAGGCTACTGTAGTGCCCAATCCCCAGGGTTGA
  GCAATATTTGTCCTTGTATTAAAAGCTGCGGAACTGGTAATGGCGTCACACGGGTAACAGGCAATCGAAA
  TTTCCTGGGATTGCTGTTCGATCCCATCGTCCAAAGCAGGGTCACCGCCTTGAAAATTACCTCCCATCCT
  ACCCCTACCCACGTTGAAAATGTGCTGACCGGCGTGCTCGATGATGGCACACTCGTGCCCAGCTCTAAGG
  CCCCTTGGGTGTTGCTGAGAATGAGTGATTACTTCTCCAGACTCCTCATCTACGAATGCAAGAAGCTGAA
  GGCCTTGGGTCTCCGCAGTTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101666 representing YP_001129386
  Red=Cloning sites Green=Tags
  
  MASRRRKLRNFLNKECIWTVNPMSGDHIKVFNACTSISPVYDPELVTSYALSVPAYNVSVAILLHKVMGP
  CVAVGINGEMIMYVVSQCVSVRPVPGRDGMALIYFGQFLEEASGLRFPYIAPPPSREHVPDLTRQELVHT
  SQVVRRGDLTNCTMGLEFRNVNPFVWLGGGSVWLLFLGVDYMAFCPGVDGMPSLARVAALLTRCDHPDCV
  HCHGLRGHVNVFRGYCSAQSPGLSNICPCIKSCGTGNGVTRVTGNRNFLGLLFDPIVQSRVTALKITSHP
  TPTHVENVLTGVLDDGTLVPSSKAPWVLLRMSDYFSRLLIYECKKLKALGLRSY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009333
• ORF Size: 1002 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009333.1, YP_001129386
• RefSeq ORF: 1002
• Locus ID: 4961502
• MW: 36.7 kDa