K4.1 (NC_009333) Virus Tagged ORF Clone

CAT#: VC101643

Myc-DDK-tagged ORF clone of viral ORF for K41 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129363

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: K4.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101643 represents NCBI reference of YP_001129363 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGTCAATGTGCTGGGTGTTGAGAGCTCATCTGGGTTTGTTGTTTTGGGTTGCGGTGATTGAGCTCT
  GTGCCGCCTCAGGACCCGCCACGATCATGGCGTCAGACTGCTGCGAAAATAGTCTGTCTAGCGCACGGTT
  GCCACCCGACAAGTTGATCTGCGGCTGGTATTGGACTTCAACTGTCTACTGCAGGCAAAAGGCCGTCATC
  TTTGTGACGCATAGTGGCAGAAAGGTTTGTGGATCCCCAGCAAAGCGGCGGACCAGGCTCCTGATGGAGA
  AACACACCGAGATTCCCCTGGCAAAAAGAGTTGCACTGCGCGCGGGTAAGGGCCTGTGTCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101643 representing YP_001129363
  Red=Cloning sites Green=Tags
  
  MWSMCWVLRAHLGLLFWVAVIELCAASGPATIMASDCCENSLSSARLPPDKLICGWYWTSTVYCRQKAVI
  FVTHSGRKVCGSPAKRRTRLLMEKHTEIPLAKRVALRAGKGLCP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009333
• ORF Size: 342 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009333.1, YP_001129363
• RefSeq ORF: 342
• Locus ID: 4961436
• MW: 12.7 kDa