ORF70 (NC_009333) Virus Tagged ORF Clone

CAT#: VC101641

Myc-DDK-tagged ORF clone of viral ORF for ORF70 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129361

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF70
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101641 represents NCBI reference of YP_001129361 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCTTTCGTGCCACTCAGTCTGTACGTTGCGAAGAAGCTGTTTAGGGCTCGGGGGTTTCGCTTCT
  GTCAGAAGCCCGGGGTCCTTGCCTTGGCCCCCGAAGTGGATCCCTGTTCAATCCAGCATGAGGTTACAGG
  TGCTGAAACACCACACGAAGAACTTCAGTACCTGCGCCAGCTCCGGGAAATCCTGTGCAGGGGATCCGAT
  CGGCTGGACAGGACCGGGATCGGTACTCTCTCCCTGTTTGGAATGCAAGCTCGCTACAGTCTGAGGGACC
  ACTTCCCCCTGCTGACCACAAAGCGCGTATTTTGGCGAGGGGTGGTCCAAGAACTGTTGTGGTTCCTGAA
  GGGTAGTACAGACAGCCGGGAGCTGAGTAGAACTGGAGTCAAGATTTGGGACAAGAATGGCAGCAGAGAG
  TTTCTTGCTGGACGGGGACTTGCACATCGAAGGGAAGGTGACCTCGGGCCTGTGTATGGCTTTCAGTGGC
  GGCATTTTGGAGCAGCCTATGTTGATGCTGACGCTGATTATACTGGCCAGGGATTTGACCAACTCAGCTA
  CATTGTGGATCTGATTAAAAATAATCCCCACGATCGACGGATAATCATGTGCGCCTGGAATCCCGCAGAT
  CTGAGTCTTATGGCACTGCCACCCTGCCACCTGCTGTGTCAGTTCTATGTGGCTGACGGAGAGCTTTCTT
  GCCAGCTGTATCAGCGAAGTGGAGACATGGGCCTTGGAGTGCCATTTAACATTGCATCCTATTCCCTTCT
  GACCTACATGCTCGCGCATGTTACTGGACTGCGGCCAGGAGAGTTTATCCACACACTGGGGGACGCACAT
  ATCTACAAAACTCACATCGAACCACTTCGCTTGCAGTTGACCAGGACACCAAGGCCATTTCCACGGCTGG
  AAATTTTGAGATCAGTATCCTCCATGGAAGAATTCACACCTGACGACTTTAGGCTGGTTGATTATTGCCC
  CCATCCTACCATTCGCATGGAGATGGCGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101641 representing YP_001129361
  Red=Cloning sites Green=Tags
  
  MFPFVPLSLYVAKKLFRARGFRFCQKPGVLALAPEVDPCSIQHEVTGAETPHEELQYLRQLREILCRGSD
  RLDRTGIGTLSLFGMQARYSLRDHFPLLTTKRVFWRGVVQELLWFLKGSTDSRELSRTGVKIWDKNGSRE
  FLAGRGLAHRREGDLGPVYGFQWRHFGAAYVDADADYTGQGFDQLSYIVDLIKNNPHDRRIIMCAWNPAD
  LSLMALPPCHLLCQFYVADGELSCQLYQRSGDMGLGVPFNIASYSLLTYMLAHVTGLRPGEFIHTLGDAH
  IYKTHIEPLRLQLTRTPRPFPRLEILRSVSSMEEFTPDDFRLVDYCPHPTIRMEMAV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009333
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009333.1, YP_001129361
• RefSeq ORF: 1011
• Locus ID: 4961481
• MW: 38.6 kDa