K15 (NC_009333) Virus Tagged ORF Clone

CAT#: VC101715

Myc-DDK-tagged ORF clone of viral ORF for K15 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129435

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: K15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101715 represents NCBI reference of YP_001129435 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACTCTTATCTTCTTTTGGAATCTTTGGCTTTGGGCTCTTTTGGTATGTTTCTGGTGCATCACCC
  TGGTGTGCGTTACTACTAATAGTATTGACACAATGGCCTCCTTGTTGGTAATGTGTATCCTCTTCGTTTC
  AGCCATCAACAAGTATACCCAAGCGATATCAAGCAATAATCCTAAGTGGCCTAGCTCCTGGCACCTCGGT
  ATTATTGCCTGCATAGTACTGAAGCTTTGGAACCTGAGCACAACTAATTCCGTGACCTATGCATGCCTGA
  TTACCACAGCAATCCTCAGCCTTGTGACCGCTTTCCTCACCCTGATAAAGCACTGCACGGCCTGCAAACT
  GCAGCTTGAGCACGGTATTCTTTTCACTTCCACATTTGCAGTACTGATGACCAATATGTTGGTTCACATG
  TCCAATACCTGGCAGTCCTCCTGGATTTTCTTTCCCATCTCATTTACCCTGTCTCTGCCTTTCCTGTACG
  CTTTCGCCACTGTGAAGACAGGCAATATAAAGTTGGTGAGCTCCGTGAGCTTCATCTGTGCCGGTCTCGT
  AATGGGTTACCCTGTATCTTGCTGTAAAACACACACGTGCACCGCGACCGCAGCCGGCCTTTCCCTCTCA
  TCTATATATCTCGGCTTTACCGGAATTATAAGTACCCTCCACAAAAGCTGGGCTCCTCCAAAGCGGGGTA
  TTCTCACATTTCTTCTGTTGCAGGGGGGGGTGTTGACAACACAGACCCTTACAACTGAACTCCTGGCTAT
  CACCTCAACCACTGGGAACATCAAGGGGCACGAAATCCTGCTCCTCGTGTGTCTCATTTTCCTCTGGTGC
  CTTTATGTTTGGCAGTCCTTCAACAAGGCTTCTCTCGTCACCGGCATGCTGCACCTGATTGCAGCCTGGT
  CACACACAGGTGGATGCGTTCAACTCGTTATGCTTCTGCCTTCAGGACTTACTAGGGGTATCTTGACTAT
  GATAATTTGTATATCAACGCTCTTCAGTACGCTCCAGGGACTTTTGGTGTTCTACCTGTATAAAGAAAAG
  AAAGTGGTCGCTGTGAACAGCTACCGGCAGCGCAGACGGCGGATTTATACTAGAGACCAAAACCTGCATC
  ACAACGACAATCATCTGGGAAACAATGTGATCAGTCCTCCTCCCCTTCCTCCCTTTTTCCGGCAGCCCGT
  TCGACTTCCCAGTCACGTGACGGACCGCGGGAGAGGCTCCCAACTGCTTAACGAGGTGGAGCTCCAGGAG
  GTGAACCGGGACCCCCCGAACGTGTTTGGCTACGCCAGCATCCTGGTGTCAGGAGCCGAGGAAAGCCGCG
  AACCGAGTCCCCAACCCGATCAGTCAGGGATGTCAATTCTGAGGGTGGATGGGGGTAGCGCGTTCCGGAT
  CGATACCGCACAGGCCGCTACGCAACCAACCGATGACCTCTACGAGGAAGTGCTGTTCCCAAGAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101715 representing YP_001129435
  Red=Cloning sites Green=Tags
  
  MKTLIFFWNLWLWALLVCFWCITLVCVTTNSIDTMASLLVMCILFVSAINKYTQAISSNNPKWPSSWHLG
  IIACIVLKLWNLSTTNSVTYACLITTAILSLVTAFLTLIKHCTACKLQLEHGILFTSTFAVLMTNMLVHM
  SNTWQSSWIFFPISFTLSLPFLYAFATVKTGNIKLVSSVSFICAGLVMGYPVSCCKTHTCTATAAGLSLS
  SIYLGFTGIISTLHKSWAPPKRGILTFLLLQGGVLTTQTLTTELLAITSTTGNIKGHEILLLVCLIFLWC
  LYVWQSFNKASLVTGMLHLIAAWSHTGGCVQLVMLLPSGLTRGILTMIICISTLFSTLQGLLVFYLYKEK
  KVVAVNSYRQRRRRIYTRDQNLHHNDNHLGNNVISPPPLPPFFRQPVRLPSHVTDRGRGSQLLNEVELQE
  VNRDPPNVFGYASILVSGAEESREPSPQPDQSGMSILRVDGGSAFRIDTAQAATQPTDDLYEEVLFPRN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009333
• ORF Size: 1467 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009333.1, YP_001129435
• RefSeq ORF: 1467
• Locus ID: 4961473
• MW: 54.3 kDa