ORF72 (NC_009333) Virus Tagged ORF Clone

CAT#: VC101710

Myc-DDK-tagged ORF clone of viral ORF for ORF72 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129430

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF72
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101710 represents NCBI reference of YP_001129430 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCGCTAATAACCCCCCATCAGGACTTCTCGATCCTACACTTTGTGAAGATCGAATCTTTTATA
  ACATCCTGGAGATTGAGCCGAGGTTCCTGACGAGTGATTCCGTGTTCGGCACCTTTCAGCAGAGTTTGAC
  TTCTCACATGAGGAAGCTTCTGGGCACTTGGATGTTCTCTGTGTGTCAGGAATACAATCTGGAGCCTAAC
  GTGGTTGCCCTGGCCCTTAACCTGCTGGACAGACTTTTGCTCATTAAGCAGGTCTCTAAAGAGCATTTTC
  AAAAAACAGGGTCCGCCTGCCTGCTCGTCGCCTCAAAATTGAGATCACTTACACCCATCTCTACATCCTC
  TCTTTGTTACGCAGCTGCAGATTCATTCTCACGCCAAGAACTCATAGACCAGGAGAAAGAACTGCTTGAG
  AAACTTGCCTGGAGAACCGAAGCCGTGTTGGCTACAGACGTCACTTCCTTTCTGCTTCTTAAATTGCTGG
  GCGGTAGCCAGCATCTCGATTTCTGGCACCACGAGGTTAATACACTCATCACGAAGGCTCTCGTCGATCC
  CAAAACCGGGTCTTTGCCCGCGAGCATCATCTCAGCCGCTGGCTGCGCCCTCTTGGTGCCTGCAAACGTG
  ATTCCACAAGATACCCACTCTGGAGGCGTCGTGCCCCAGTTGGCCTCAATACTGGGTTGTGACGTTAGCG
  TCTTGCAGGCTGCGGTCGAACAAATACTGACCTCCGTGTCTGACTTCGATCTGCGCATCCTTGACAGCTA
  T
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101710 representing YP_001129430
  Red=Cloning sites Green=Tags
  
  MATANNPPSGLLDPTLCEDRIFYNILEIEPRFLTSDSVFGTFQQSLTSHMRKLLGTWMFSVCQEYNLEPN
  VVALALNLLDRLLLIKQVSKEHFQKTGSACLLVASKLRSLTPISTSSLCYAAADSFSRQELIDQEKELLE
  KLAWRTEAVLATDVTSFLLLKLLGGSQHLDFWHHEVNTLITKALVDPKTGSLPASIISAAGCALLVPANV
  IPQDTHSGGVVPQLASILGCDVSVLQAAVEQILTSVSDFDLRILDSY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009333
• ORF Size: 771 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009333.1, YP_001129430
• RefSeq ORF: 771
• Locus ID: 4961471
• MW: 28.1 kDa