ORF58 (NC_009333) Virus Tagged ORF Clone

CAT#: VC101695

Myc-DDK-tagged ORF clone of viral ORF for ORF58 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129415

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF58
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101695 represents NCBI reference of YP_001129415 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCAGGCTGGACTCTGAACGCGCCCTGAGCCTGTTCAGCTACCTGTCCGGCACCCTGGCCGCTACCC
  CCTTCTTGTGGTGCTTCATCTTTAAAGCCCTGTATTCCTTCACACTGTTTACGACCGAGATTACTGCAGT
  CTTCTTTTGGTCATTGCCTGTTACCCACCTGGCCCTCATTTGTATGTGCCTGTGTCCTGCTGCACAAAAG
  CAACTGGACCGGAGGCTTGAGTGGATTTGTGCCAGCGCCGTTTTTGCTGCCGTGGTCTGTGCCGCCTTTT
  CAGGGTTTACATTCTCCAGAGTACCATTTATTCCTGGGTTGTGTGTACTGAATTGCCTGCTGCTTTTGCC
  CTACCCCCTGGCCACCGCCACAGCAGTATACCAGGCGCCTCCAATAGTGCATAGGTATTATGAACTGGGA
  TTTTGTGGCGCCTTCATGGTTTATTATCTGTTGCTTTTCAAGAAGGTCTTTGTTTCCGGGGTGTTTTGGC
  TCCCCTTCATAGTGTTTCTCGTTGGAGGTCTGCTGGCTTTCCGACACCTGGAGCAACACGTATACATTCG
  AGCGGGGATGCAGAGGAGGCGGGCTATCTTTATCATGCCTGGCAAGTATATCACGTACTCCGTGTTTCAG
  GCTTGGGCTTATTGTCGGCGAGAGGTGGTAGTCTTCGTGACCTTGCTGCTCGCTACCCTGATCTCTACCG
  CATCCATCGGCCTCCTGACCCCCGTGCTTATTGGTCTGGATAAGTACATGACACTCTTCTACGTAGGCCT
  GCTTAGTTGTGTTGGTGTTTCCGTGGCGAGTAGACGAGCTTTGTTCGTCCTGCTTCCTCTGGCAGCAGTC
  CTGCTGACTCTCGTGCATATTTTGGGAAGCGGCCCAGATATGCTCCTTGTGAGGTCTTGCCTCTGCTGTC
  TGTTCCTGGTGTCCATGCTTGCCGCAATGGGTGTTGAGATACAACTTATCAGGAGAAAGCTGCACAGAGC
  TCTGAACGCCCCCCAGATGGTGCTCGCCCTTTGCACTGTCGGTAATCTGTGCATAAGCTGCCTGCTCAGC
  GTGATAAACAAGGTTGTCGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101695 representing YP_001129415
  Red=Cloning sites Green=Tags
  
  MCRLDSERALSLFSYLSGTLAATPFLWCFIFKALYSFTLFTTEITAVFFWSLPVTHLALICMCLCPAAQK
  QLDRRLEWICASAVFAAVVCAAFSGFTFSRVPFIPGLCVLNCLLLLPYPLATATAVYQAPPIVHRYYELG
  FCGAFMVYYLLLFKKVFVSGVFWLPFIVFLVGGLLAFRHLEQHVYIRAGMQRRRAIFIMPGKYITYSVFQ
  AWAYCRREVVVFVTLLLATLISTASIGLLTPVLIGLDKYMTLFYVGLLSCVGVSVASRRALFVLLPLAAV
  LLTLVHILGSGPDMLLVRSCLCCLFLVSMLAAMGVEIQLIRRKLHRALNAPQMVLALCTVGNLCISCLLS
  VINKVVG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009333
• ORF Size: 1071 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009333.1, YP_001129415
• RefSeq ORF: 1071
• Locus ID: 4961480
• MW: 39.7 kDa