ORF22 (NC_009333) Virus Tagged ORF Clone

CAT#: VC101655

Myc-DDK-tagged ORF clone of viral ORF for ORF22 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129375

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101655 represents NCBI reference of YP_001129375 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGGCCTGGCGTTTCTCGCGGCACTCGCTTGTTGGCGATGTATCAGCCTGACCTGTGGCGCAACCG
  GAGCTTTGCCCACCACCGCTACCACAATCACAAGGTCTGCCACCCAATTGATCAACGGGCGAACCAATCT
  CTCTATCGAGCTCGAGTTCAACGGCACAAGTTTCTTTCTGAATTGGCAGAATCTGTTGAACGTTATCACT
  GAGCCTGCACTCACAGAATTGTGGACTAGTGCCGAGGTGGCTGAGGACTTGCGAGTGACTCTGAAGAAGC
  GACAGTCACTGTTCTTTCCAAACAAAACCGTGGTAATCTCCGGAGACGGTCACCGGTACACATGTGAGGT
  CCCAACGAGTAGTCAGACTTACAACATTACCAAGGGTTTCAACTATTCAGCTCTGCCAGGCCATCTCGGG
  GGTTTCGGGATCAACGCACGCCTGGTGCTTGGCGATATCTTTGCCTCCAAATGGAGTCTCTTCGCCAGGG
  ACACACCAGAGTACCGCGTGTTTTACCCTATGAACGTTATGGCAGTGAAGTTCTCAATCTCTATTGGCAA
  CAATGAGAGCGGCGTCGCCCTCTACGGAGTGGTGAGTGAAGATTTTGTAGTTGTGACCCTCCACAACAGG
  TCCAAAGAGGCTAATGAGACCGCTTCTCACCTGCTCTTCGGACTTCCAGACTCACTCCCCTCACTGAAGG
  GACATGCCACTTACGATGAATTGACGTTTGCCCGGAACGCCAAGTATGCACTCGTGGCTATCCTGCCCAA
  GGACAGCTATCAGACCCTGCTGACTGAAAACTACACGCGGATTTTCCTGAACATGACCGAGTCTACACCG
  CTGGAATTCACTAGAACCATCCAGACTCGCATCGTCTCAATAGAAGCTCGGCGCGCATGTGCAGCCCAGG
  AGGCTGCACCTGACATATTCCTTGTGTTGTTCCAGATGCTCGTAGCACATTTCCTGGTCGCGAGAGGAAT
  TGCCGAGCACAGGTTCGTTGAGGTGGACTGTGTCTGTCGCCAATACGCCGAACTGTATTTCTTGAGACGG
  ATATCCAGGCTCTGCATGCCGACATTCACCACAGTCGGCTACAACCATACTACCTTGGGCGCCGTTGCAG
  CCACACAGATCGCTAGGGTCTCTGCCACCAAACTCGCAAGCCTCCCAAGGTCCTCCCAGGAGACCGTGTT
  GGCTATGGTGCAACTCGGAGCACGGGACGGCGCGGTTCCTTCCTCTATACTGGAGGGTATAGCAATGGTA
  GTTGAGCACATGTATACTGCCTACACATACGTCTACACTTTGGGCGACACCGAACGGAAACTGATGCTTG
  ATATCCACACTGTGCTTACCGATTCCTGTCCCCCCAAGGACTCTGGGGTGAGTGAAAAGCTGCTCCGGAC
  CTACCTTATGTTTACGAGTATGTGCACAAACATCGAACTGGGTGAAATGATTGCAAGATTCAGTAAACCA
  GATTCCCTTAACATATACCGCGCATTCAGTCCTTGTTTCCTGGGACTGCGATATGATCTGCACCCGGCCA
  AGCTTAGGGCCGAAGCCCCTCAGTCCAGTGCTCTGACGCGCACTGCTGTTGCACGGGGAACTTCCGGATT
  TGCTGAACTCCTGCATGCTCTTCACCTTGACTCCCTGAACCTCATTCCCGCCATTAACTGTAGCAAGATC
  ACTGCCGACAAGATCATAGCGACCGTACCACTGCCCCACGTTACATACATTATCTCCTCCGAGGCCCTGA
  GCAATGCGGTGGTGTATGAGGTGTCTGAGATTTTTTTGAAATCCGCCATGTTTATATCAGCTATTAAACC
  AGACTGCTCAGGGTTTAACTTTTCACAGATCGACCGGCATATTCCCATAGTTTACAACATCTCAACCCCT
  CGCCGGGGATGCCCTCTGTGTGATTCAGTGATCATGAGTTACGACGAAAGCGATGGTCTGCAGTCACTGA
  TGTATGTGACAAATGAACGAGTGCAGACTAATCTGTTCCTGGATAAGTCCCCATTCTTTGACAACAACAA
  CCTGCACATCCATTACCTGTGGCTGAGGGATAATGGTACCGTGGTTGAGATTCGGGGAATGTACCGAAGA
  AGAGCCGCATCTGCCCTTTTCCTGATCCTCAGTTTCATTGGGTTCAGTGGGGTGATATATTTTCTCTACA
  GACTGTTCTCTATTCTGTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101655 representing YP_001129375
  Red=Cloning sites Green=Tags
  
  MQGLAFLAALACWRCISLTCGATGALPTTATTITRSATQLINGRTNLSIELEFNGTSFFLNWQNLLNVIT
  EPALTELWTSAEVAEDLRVTLKKRQSLFFPNKTVVISGDGHRYTCEVPTSSQTYNITKGFNYSALPGHLG
  GFGINARLVLGDIFASKWSLFARDTPEYRVFYPMNVMAVKFSISIGNNESGVALYGVVSEDFVVVTLHNR
  SKEANETASHLLFGLPDSLPSLKGHATYDELTFARNAKYALVAILPKDSYQTLLTENYTRIFLNMTESTP
  LEFTRTIQTRIVSIEARRACAAQEAAPDIFLVLFQMLVAHFLVARGIAEHRFVEVDCVCRQYAELYFLRR
  ISRLCMPTFTTVGYNHTTLGAVAATQIARVSATKLASLPRSSQETVLAMVQLGARDGAVPSSILEGIAMV
  VEHMYTAYTYVYTLGDTERKLMLDIHTVLTDSCPPKDSGVSEKLLRTYLMFTSMCTNIELGEMIARFSKP
  DSLNIYRAFSPCFLGLRYDLHPAKLRAEAPQSSALTRTAVARGTSGFAELLHALHLDSLNLIPAINCSKI
  TADKIIATVPLPHVTYIISSEALSNAVVYEVSEIFLKSAMFISAIKPDCSGFNFSQIDRHIPIVYNISTP
  RRGCPLCDSVIMSYDESDGLQSLMYVTNERVQTNLFLDKSPFFDNNNLHIHYLWLRDNGTVVEIRGMYRR
  RAASALFLILSFIGFSGVIYFLYRLFSILY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009333
• ORF Size: 2190 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009333.1, YP_001129375
• RefSeq ORF: 2190
• Locus ID: 4961506
• MW: 81.3 kDa