ORF32 (NC_009333) Virus Tagged ORF Clone

CAT#: VC101665

Myc-DDK-tagged ORF clone of viral ORF for ORF32 [Human herpesvirus 8], codon optimized for human cell expression, YP_001129385

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101665 represents NCBI reference of YP_001129385 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCACACGCCATAAATGAGAGATACGTTGGCCCCAGATGTCACCGGCTTGCCCATGTCGTTCTCC
  CCAGAACTTTTCTTCTTCACCACGCCATACCCCTGGAGCCGGAGATTATCTTTTCAACATATACAAGGTT
  TAGCAGGAGCCCAGGTAGTTCACGGAGGCTTGTGGTATGCGGCAAGCGGGTATTGCCCGGGGAAGAGAAC
  CAGCTGGCCTCATCCCCCTCAGGACTGGCCCTGAGCCTTCCCCTTTTCTCTCACGATGGGAATTTTCACC
  CTTTTGATATCTCCGTGCTGCGCATAAGCTGTCCTGGAAGCAACCTTTCTTTGACGGTTCGCTTCTTGTA
  CTTGAGTCTCGTCGTCGCTATGGGTGCTGGCAGAAATAACGCACGAAGTCCAACCGTGGATGGGGTAAGC
  CCTCCTGAGGGTGCCGTCGCTCATCCGCTCGAAGAACTGCAGCGGCTGGCGCGGGCCACACCAGATCCAG
  CCCTTACCCGCGGCCCACTGCAAGTCCTGACAGGTTTGCTCCGAGCAGGCTCAGACGGCGATCGAGCTAC
  TCACCACATGGCTCTCGAAGCACCCGGCACGGTGCGAGGCGAAAGTCTGGATCCCCCAGTTTCACAGAAA
  GGACCAGCTAGAACCCGGCACCGCCCCCCCCCGGTTCGGTTGTCATTCAATCCTGTGAACGCCGACGTGC
  CCGCCACTTGGAGAGATGCTACCAACGTGTATAGCGGAGCCCCCTACTATGTCTGCGTGTATGAGCGGGG
  TGGGCGGCAGGAAGATGATTGGCTCCCGATCCCACTCAGCTTCCCTGAAGAACCAGTGCCACCTCCCCCT
  GGGCTCGTGTTCATGGACGACCTGTTTATCAATACAAAGCAGTGCGACTTTGTCGATACTCTGGAAGCTG
  CCTGCAGAACACAGGGATATACCTTGCGGCAGCGCGTCCCAGTTGCCATCCCACGGGACGCCGAAATCGC
  AGATGCCGTTAAAAGCCACTTTCTCGAAGCTTGCCTCGTGCTGCGCGGGCTGGCATCCGAAGCTTCTGCA
  TGGATACGCGCTGCCACTTCTCCCCCACTGGGACGGCACGCATGTTGGATGGACGTGCTGGGCCTGTGGG
  AAAGCAGACCCCATACTCTCGGGCTTGAACTGCGCGGAGTGAACTGTGGAGGCACAGACGGAGATTGGTT
  GGAAATCCTCAAACAGCCAGACGTCCAAAAGACGGTGAGCGGCTCCCTGGTGGCATGCGTGATAGTCACG
  CCTGCGTTGGAGGCCTGGCTTGTGCTGCCTGGCGGCTTCGCAATCAAGGGTAGATATAGAGCAAGCAAGG
  AAGACCTCGTATTTATCCGGGGGAGATATGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101665 representing YP_001129385
  Red=Cloning sites Green=Tags
  
  MDAHAINERYVGPRCHRLAHVVLPRTFLLHHAIPLEPEIIFSTYTRFSRSPGSSRRLVVCGKRVLPGEEN
  QLASSPSGLALSLPLFSHDGNFHPFDISVLRISCPGSNLSLTVRFLYLSLVVAMGAGRNNARSPTVDGVS
  PPEGAVAHPLEELQRLARATPDPALTRGPLQVLTGLLRAGSDGDRATHHMALEAPGTVRGESLDPPVSQK
  GPARTRHRPPPVRLSFNPVNADVPATWRDATNVYSGAPYYVCVYERGGRQEDDWLPIPLSFPEEPVPPPP
  GLVFMDDLFINTKQCDFVDTLEAACRTQGYTLRQRVPVAIPRDAEIADAVKSHFLEACLVLRGLASEASA
  WIRAATSPPLGRHACWMDVLGLWESRPHTLGLELRGVNCGGTDGDWLEILKQPDVQKTVSGSLVACVIVT
  PALEAWLVLPGGFAIKGRYRASKEDLVFIRGRYG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009333
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_009333.1, YP_001129385
• RefSeq ORF: 1362
• Locus ID: 4961479
• MW: 49.5 kDa