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Antibodies
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Chicken Polyclonal Anti-Beta-actin Antibody
| Applications | WB |
| Reactivities | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Immunogen | Beta-actin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human beta-actin. |
GFP (Ads. to Hu, Ms, Rt Serum Proteins) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IF, WB |
| Reactivities | A. victoria |
| Immunogen | Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria |
HA Epitope Tag (YPYDVPDYA) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IF, IHC, WB |
| Immunogen | Synthetic peptide containing the Influenza Hemaglutinin epitope (YPYDVPDYA) conjugated to Keyhole Limpet Hemocyanin (KLH). |
c-Myc (MYC) chicken polyclonal antibody, FITC
| Applications | ELISA, IF, IHC, WB |
| Reactivities | Human |
| Conjugation | FITC |
| Immunogen | Synthetic peptide containing the Human c-myc epitope (i.e., EQKLISEEDL) conjugated to KLH. After repeated injections, immune eggs were collected, from which the IgY fractions were prepared. |
Goat IgG (H+L chain) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA. Western Blot (1/1000). Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose. |
| Reactivities | Goat |
| Immunogen | Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant. Production. After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (whole protein). |
Choline Acetyltransferase (CHAT) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC |
| Reactivities | Human, Mouse |
| Immunogen | Synthetic peptide KLH conjugated corresponding to a region of the Choline Acetyltransferase gene product shared between the Human (P28329) and Mouse (Q03059) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks and then affinity-purified using a peptide column. |
Amyloid beta (N-term) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC |
| Reactivities | Human, Mouse |
| Immunogen | Hens were immunized with a synthetic peptide KLH conjugated corresponding to DAE FRH DSG YEV HHQ KL, residues 1-17 of the Amyloid beta-peptide (Amyloid Precursor Protein residues #672-688). After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. |
Myelin Protein Zero (MPZ) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | Two antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (NP_032649) and Human (NP_000521) gene products. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of both of these affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Tau (MAPT) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | Antipeptide antibodies were generated in chickens against two sequences shared between the Mouse (P10637.3) and Human (P10636.5) gene products. Neither of these sequences contain phosphorylated serine or threonine residues, so these tau antibodies recognize both the euphosphorylated and hyperphosphorylated forms of Tau. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of both of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Amyloid beta A4 protein / APP (433-452) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC |
| Reactivities | Human, Mouse, Rat |
| Immunogen | Chickens were immunized with a Keyhole Limpet Hemocyanin (KLH) conjugate of the synthetic peptide QHF QEK VES LEQ EAA NER QQ, which corresponds to residues #433-452 of the Human gene product (NP_000475.1). This sequence is also conserved in the Mouse and Rat APP gene products, as well. |
GDNF Receptor alpha 2 (GFRA2) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IF, WB |
| Reactivities | Human |
| Immunogen | Recombinant His-tagged human GFRa-2 protein produced using CHO-based. For production of hGFRa-2, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant. |
GFRA4 / GDNFR-alpha 4 chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IF, WB |
| Reactivities | Human |
| Immunogen | Recombinant His-tagged human GFRa-4 protein produced using CHO-based. For production of hGFRa-4, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant. |
GAP43 chicken polyclonal antibody
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Conjugation | Unconjugated |
Neuroglobin (NGB) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IHC, WB |
| Reactivities | Canine, Human, Rat |
| Immunogen | Recombinant human neuroglobin. The Human Neuroglobin is created as a recombinant protein produced in E. coli. It is the 17 kDa protein containing 150 amino acid residues of the Human Neuroglobin and one extra AA, N-terminal methionin. The amino acid sequence of the recombinant human neuroglobin is 100% homologous with the amino acid sequence of the human neuroglobin. |
Chicken Polyclonal ApoA1 Antibody
| Applications | IF, WB |
| Reactivities | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Immunogen | ApoA1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ApoA1. The immunogen is located within the first 50 amino acids of ApoA1. |
ATPase (NA/K) chicken polyclonal antibody, Ig Fraction
| Applications | ELISA, IHC, WB |
| Reactivities | Canine |
| Immunogen | ATPase, Sodium, Potassium [Canine Kidney] |
GFP (Ads. to Hu, Ms, Rt Serum Proteins) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IF, WB |
| Reactivities | A. victoria |
| Immunogen | Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria |
GFP chicken polyclonal antibody, Aff - Purified
| Applications | IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | Purified recombinant Green Fluorescent Protein (GFP) |
beta III Tubulin (TUBB3) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse, Rat |
| Immunogen | Synthetic peptide corresponding to a region of the Tuj1 gene product shared between the Rat (AAM28438, NCBI) and Human (AAL28094, NCBI) gene products. |
beta III Tubulin (TUBB3) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Mouse |
| Immunogen | TUBB3 / Tubulin Beta 3 antibody was raised against three synthetic peptides corresponding to different regions of the beta-Tubulin 3 (KLH). |
Complement C3 (C3) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, FC, WB |
| Reactivities | Human |
| Immunogen | Purified Human Complement Component 3a (C3a) peptide. |
GFAP chicken polyclonal antibody, Serum
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | GFAP antibody was raised against full-length recombinant mouse GFAP |
GFP chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Immunogen | Purified recombinant Green Fluorescent Protein (GFP) emulsified in Freund's adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Purified antibody preparations were then mixed with Glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with Thimerosal, and then filter-sterilized. |
Human IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | ELISA: a 1/300,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethyl-benzidine as substrate. Western Blot (1/5000). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and ELISA analysis. |
| Reactivities | Human |
| Conjugation | HRP |
| Immunogen | Purified, non-denatured Human IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase. |
Mouse IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | ELISA: a 1/70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot: 1/200 -1/5000. Immunocytochemistry. Immunohistochemistry: 1/250 - 1/5000. Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Purified, non-denatured Mouse IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Horseradish peroxidase. |
Rabbit IgG (H+L chain) chicken polyclonal antibody, FITC
| Applications | Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues. |
| Reactivities | Rabbit |
| Conjugation | FITC |
| Immunogen | Purified, non-denatured Rabbit IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains) and then conjugated with Fluorescein isothiocyanate. |
Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | ELISA: a 1/20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot (1/5000). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis. |
| Reactivities | Rabbit |
| Conjugation | HRP |
| Immunogen | Purified, non-denatured Rabbit IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains) and then conjugated to Horseradish Peroxidase. |
CNPase (CNP) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | Synthetic peptide KLH conjugated corresponded to a region of the CNPase gene product shared between the Human (NP_149124) and Mouse (P16330) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks. |
NSE (ENO2) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse, Rat |
| Immunogen | Chickens were immunized with two synthetic peptide KLH conjugates corresponding to different regions of the NSE-2 gene product, but are shared between the Human (NP_001966, NCBI) and Rat (AAA41119) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of each of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Peripherin chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Rat |
| Immunogen | Full-length recombinant Peripherin protein. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. |
Synaptotagmin 1 (SYT1) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | Four different antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (NP_033332) and Human (NP_005630) gene products. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.4 mg/ml. Finally, equal volumes of each of the four affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. Antibodies were affinity-purified and mixed in equal concentrations. |
beta III Tubulin (TUBB3) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Rat |
| Immunogen | Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Beta-Tubulin 3 gene product, but are shared between the Human (AAL28094) and Rat (AAM28438) protein sequences. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Tyrosine Hydroxylase (TH) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | Two antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (P24529) and Human (P07101) gene products. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of both of these affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Vimentin (VIM) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Human, Mouse |
| Immunogen | Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Vimentin gene product, but are shared between the Human (NP_003371) and Mouse (NP_035831) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Doublecortin (DCX) chicken polyclonal antibody, Purified
| Applications | IF, IHC |
| Reactivities | Human, Mouse |
| Immunogen | Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the Doublecortin gene product, but are shared between the Human (CAA06617.1, NCBI) and Mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
mGluR1a / GRM1a chicken polyclonal antibody, Purified
| Applications | IF, IHC |
| Reactivities | Human, Mouse |
| Immunogen | Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 1 gene product, but are shared between the human (NP_000829.2, NCBI) and mouse (NP_058672.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
Metabotropic Glutamate Receptor 5 (GRM5) chicken polyclonal antibody, Purified
| Applications | IF, IHC |
| Reactivities | Human, Mouse |
| Immunogen | Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 5 gene product, but are shared between the Human (NP_001137303.1, NCBI) and Mouse (Q3UVX5.2, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. |
GAP43 (C-term) chicken polyclonal antibody, Aff - Purified
| Applications | IF, IHC, WB |
| Reactivities | Mouse |
| Immunogen | Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of Mouse GAP-43 coupled to keyhole limpet hemocyanin. Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1/10 with PBS containing BSA as a carrier. Finally, the antibody preparation was filter-sterilized. |
Pirimiphos chicken polyclonal antibody, Purified
| Applications | AP, ELISA |
| Immunogen | BSA-Pirimiphos-methyl Conjugate. Chicken egg yolk, purified by PEG extraction. |
RET chicken polyclonal antibody, Aff - Purified
| Applications | ELISA, IF, WB |
| Reactivities | Human |
| Immunogen | Recombinant His-tagged extracellular fragment of human RET protein produced using CHO-based. The extracellular fragment of hRET was expressed and secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant. |
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