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Rabbit IgG sheep polyclonal antibody, Purified
| Applications | ELISA. Western Blot: 1/1000. Immunohistochemistry on Frozen Sections. |
| Reactivities | Rabbit |
| Immunogen | Purified Rabbit IgG |
Secondary Antibodies
DownloadSheep/Goat IgG donkey polyclonal antibody, AP
| Applications | Western blot. ELISA: 1/2000-1/5000. Immunohistochemistry on Frozen Sections. |
| Reactivities | Goat, Sheep |
| Conjugation | AP |
| Immunogen | Purified IgG from a Sheep serum pool |
Chicken IgM (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/10 - 1/40, depending on the method used. |
| Reactivities | Chicken |
| Conjugation | FITC |
| Immunogen | Purified IgM isolated from chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Bovine IgG1 (subclass specific) rabbit polyclonal antibody, Serum
| Applications | Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG1 in bovine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of bovine IgG1 from serum or plasma. Antibody titre: Precipitin titre not less then 1/16 when tested against pooled normal bovine serum in agar block immunodiffusion titration. Recommended Dilutions: Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
| Reactivities | Bovine |
| Immunogen | Pools of purified normal IgG1 isolated from pooled Bovine Serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Bovine IgA (secretory) rabbit polyclonal antibody, Serum
| Applications | This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgA in Bovine serum, milk or other body fluids or to determine its concentration. Can also be used to prepare an immunoadsorbent for the purification of Bovine IgA from serum or exocrine secretions. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude such use if proper controls are include. Recommneded Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal Bovine serum in agar block titration. |
| Reactivities | Bovine |
| Immunogen | Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgA (Secretory component) goat polyclonal antibody, Serum
| Applications | Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. Can be used in precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence secretory component in monkey serum or other body fluids and to determine its concentration. Recommended dilutions: Immunoelectrophoresis: use 2 μl serum or equivalent against 120 μl antiserum. Double radial immunodiffusion: use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. |
| Reactivities | Chimpanzee, Monkey |
| Immunogen | Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Purified free secretory component isolated from pooled rhesus monkey milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Feline IgG (H+L chain) goat polyclonal antibody, Serum
| Applications | Can be used: • In precipitating techniques as Immunoelectrophoresis and Double Radial Immunodiffusion to identify the presence of IgG. • As secondary antibody to precipitate the immunoglobulin in normal Cat serum. • To prepare an immunoadsorbent for the production of Ig-free Cat serum, plasma or non-Ig preparations. Directions for use: Immunoelectrophoresis: Use 2 μl or equivalent against 120 μl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
| Reactivities | Feline |
| Immunogen | Purified normal IgG isolated from pooled cat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgA (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/2000. |
| Reactivities | Canine |
| Immunogen | Purified IgA isolated from dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgG (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000. |
| Reactivities | Canine |
| Immunogen | Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgM (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/3000. |
| Reactivities | Canine |
| Immunogen | Purified IgM isolated from Dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgG rabbit polyclonal antibody, Ig Fraction
| Applications | Immunoprecipitation. Immunodiffusion. |
| Reactivities | Human |
Mouse IgG (H+L chain) rabbit polyclonal antibody, Ig Fraction
| Applications | Immunoprecipitation. Immunodiffusion. Ouchterlony Test: Sample size: 15 µl. Distance between wells: 5 mm. Test Time: 5 days. Temperature: 37°C. Samples Tested: Normal mouse Serum (NMS), Purified mouse IgG, Normal human serum (NHS), Purified human IgG. Results: Specificity: a single band against NMS was detected at dilutions of 1/4 and 1/8 which is homogeneous with band to purified mouse IgG. No bands were detected against human IgG at 5 mg/ml or NHS at 1/4 dilution. Protein content: Biuret Assay 14 mg/ml. |
| Reactivities | Mouse |
Goat IgG (H+L chain) mouse polyclonal antibody, Serum
| Applications | ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunochemistry: 1/1,000-1/5,000. |
| Reactivities | Goat |
| Immunogen | Goat IgG whole molecule. |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin
| Applications | ELISA. Western Blot (1/5000). Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA using HRP-labeled streptavidin. A 1/500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with Tetramethylbenzidine as the substrate. |
| Reactivities | Chicken |
| Conjugation | Biotin |
| Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
Human IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | ELISA: a 1/300,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethyl-benzidine as substrate. Western Blot (1/5000). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and ELISA analysis. |
| Reactivities | Human |
| Conjugation | HRP |
| Immunogen | Purified, non-denatured Human IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase. |
Mouse IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | ELISA: a 1/70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot: 1/200 -1/5000. Immunocytochemistry. Immunohistochemistry: 1/250 - 1/5000. Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Purified, non-denatured Mouse IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Horseradish peroxidase. |
Rabbit IgG (H+L chain) chicken polyclonal antibody, FITC
| Applications | Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues. |
| Reactivities | Rabbit |
| Conjugation | FITC |
| Immunogen | Purified, non-denatured Rabbit IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains) and then conjugated with Fluorescein isothiocyanate. |
Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | ELISA: a 1/20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot (1/5000). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis. |
| Reactivities | Rabbit |
| Conjugation | HRP |
| Immunogen | Purified, non-denatured Rabbit IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains) and then conjugated to Horseradish Peroxidase. |
Mouse IgG3 goat polyclonal antibody, FITC
| Applications | Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl. Immunocytochemistry: Neat-1/10. Immunohistochemistry on Frozen Sections. |
| Reactivities | Mouse |
| Conjugation | FITC |
| Immunogen | Mouse IgG3 paraproteins |
Mouse IgG3 goat polyclonal antibody, HRP
| Applications | ELISA: 1/4000-1/8000. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Mouse IgG3 paraproteins. |
Mouse IgA (alpha chain specific) goat polyclonal antibody, AP
| Applications | ELISA: 1/2,000-1/4,000. |
| Reactivities | Mouse |
| Conjugation | AP |
| Immunogen | Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins. |
Mouse IgA (alpha chain specific) goat polyclonal antibody, Texas Red
| Applications | FLISA: 1/100-1/400. Flow Cytometry. Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | Texas Red |
| Immunogen | Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins. |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, FITC
| Applications | FLISA. |
| Reactivities | Mouse |
| Conjugation | FITC |
| Immunogen | Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins. |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP
| Applications | ELISA: 1/4,000-1/8,000. Immunoblotting. Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins. |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Texas Red
| Applications | FLISA: 1/100–1/400. |
| Reactivities | Mouse |
| Conjugation | Texas Red |
| Immunogen | Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins. |
Duck IgA+IgG+IgM (H+L chain) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal Duck serum. Directions for Use: In immunoelectrophoresis use 2 µl or equivalent against 120 µl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/64 when tested against normal Duck serum in agar block titration. |
| Reactivities | Duck |
| Immunogen | Purified normal Ig fractions isolated from pooled Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Duck IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | ELISA. Immunocytochemistry. (In)direct Immunofluorescence. Immunohistochemistry on Frozen Sections. Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of duck origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Duck |
| Conjugation | FITC |
| Immunogen | Purified normal Ig fractions isolated from pooled Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Duck IgG (H+L chain) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in Immunoelectrophoresis and Double Radial Immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal duck serum; to prepare an immunoadsorbent for the production of Ig-free duck serum, plasma or non-Ig preparations. Directions for Use: In immunoelectrophoresis use 2 µl or equivalent against 120 µl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/64 when tested against normal Duck serum in agar block titration. |
| Reactivities | Duck |
| Immunogen | Purified normal Ig fractions isolated from pooled Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea Pig IgG (Fc specific) sheep polyclonal antibody, FITC
| Applications | ELISA. (In)direct immunofluorescence. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Can be used in direct staining of cytoplasmic IgG in fixed guinea pig cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of guinea pig in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Guinea Pig |
| Conjugation | FITC |
| Immunogen | Purified normal IgG isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (Fc specific) rabbit polyclonal antibody, FITC
| Applications | ELISA. Immunocytochemistry. (In)direct Immunofluorescence. Immunohistochemistry on Frozen Sections. Can be used In direct staining of cytoplasmic IgG in fixed sheep cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Sheep |
| Conjugation | FITC |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000. Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (Fc specific) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (Fc specific) rabbit polyclonal antibody, FITC
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Frozen Sections. Can be used in immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Sheep |
| Conjugation | FITC |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (H+L chain) rabbit polyclonal antibody, FITC
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Frozen Sections. Can be used to identify IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Sheep |
| Conjugation | FITC |
| Immunogen | Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | ELISA. Immunocytochemistry. Immunofluorescence. Immunohistochemistry Frozen Sections. Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of swine origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Porcine |
| Conjugation | FITC |
| Immunogen | Purified normal IgG isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (H+L chain) rabbit polyclonal antibody, TRITC
| Applications | ELISA. Immunocytochemistry. Immunofluorescence. Immunohistochemistry Frozen Sections. Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two Nordic immunoconjugates with different labels but originating from the same host animal species are used for direct two-colour staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80. |
| Reactivities | Porcine |
| Conjugation | TRITC |
| Immunogen | Purified normal IgG isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgA (secretory) rabbit polyclonal antibody, Serum
| Applications | Precipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in swine milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgA from serum or exocrine secretions. Antisera to IgA or sIgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included. Recommended Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody Titre: Precipitin titre not less than 1/32 when tested against pooled normal swine serum in agar-block immunodiffusion titration. |
| Reactivities | Porcine |
| Immunogen | Highly purified secretory IgA isolated from pooled Swine milk. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgG goat polyclonal antibody, AP, Aff - Purified
| Applications | ELISA: 1/10000. Western Blot: 1/5000-1/10000, Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | AP |
| Immunogen | Purified Mouse IgG fraction |
Mouse IgG goat polyclonal antibody, HRP, Aff - Purified
| Applications | ELISA: 1/10000. Western Blot: 1/5000-1/10000, Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Purified Mouse IgG fraction |
USD 355.00
3 Days
Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, AP, Aff - Purified
| Applications | Anti-Mouse IgG1 Alkaline Phosphatase Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods. Recommended Dilutions: ELISA: 1/10,000-1/40,000. Western Blot: 1/200-1/2,000. Immunohistochemistry: 1/200-1/1,000. |
| Reactivities | Mouse |
| Conjugation | AP |
| Immunogen | Native Mouse IgG1 heavy chain Protein. |
USD 305.00
3 Days
Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, Biotin, Aff - Purified
| Applications | Anti-Mouse IgG1 Biotin Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Native Mouse IgG1 heavy chain Protein. |
USD 305.00
3 Days
Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, HRP, Aff - Purified
| Applications | Mouse IgG1 Peroxidase Secondary antibody reagents are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/20,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Mouse |
| Conjugation | HRP |
| Immunogen | Native Mouse IgG1 heavy chain Protein. |
USD 265.00
3 Days
Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, Aff - Purified
| Applications | Anti-Mouse IgG1 secondary antibody is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays. Recommended Dilutions: ELISA: 1/40,000-1/200,000. Western Blot: 1/4,000-1/20,000. Immunohistochemistry: 1/2,000-1/10,000. |
| Reactivities | Mouse |
| Immunogen | Native Mouse IgG1 heavy chain Protein. |
Human IgA (secretory) sheep polyclonal antibody, Serum
| Applications | Precipitation. Can be used in precipitating as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgA in Human serum, milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of Human IgA from serum or exocrine secretions. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude such use if proper controls are include. Recommended Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody Titre: Precipitin titre not less than 1/64 when tested against pooled normal human serum in agar-block immunodiffusion titration. |
| Reactivities | Human |
| Immunogen | Highly purified secretory IgA isolated from pooled Human milk. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgG (H+L chain) rabbit polyclonal antibody, AP
| Applications | Suitable for use in Immunoblotting (Western or dot blot), ELISA, Immunohistochemistry and other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/2,000-1/10,000. Western blot: 1/500-1/2,500. Immunohistochemistry: 1/200-1/1,000. |
| Conjugation | AP |
| Immunogen | Mouse IgG whole molecule |
Mouse IgG (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/300,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Conjugation | Biotin |
| Immunogen | Mouse IgG whole molecule. |
Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: Western blot: 1/1,000-1/10,000. ELISA: 1/10,000-1/50,000. Immunohistochemistry: 1/500-1/2500. |
| Conjugation | HRP |
| Immunogen | Chicken IgY/IgG whole molecule |
Bovine IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified
| Applications | ELISA: 1:20,000 - 1:100,000. Immunohistochemistry: 1:1,000 - 1,5,000. Western blotting: 1:2,000 - 1;10,000. Suitable for most immunological methods requiring lot-to-lot consistency, high titer and specificity. |
| Reactivities | Bovine |
| Immunogen | Bovine IgG F(c) fragment. |
Canine IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:40,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Dog IgG F(c) fragment |
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