Secondary Antibodies

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Sheep IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in sheep serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/50-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Reactivities Sheep
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Sheep IgM (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000.
Reactivities Sheep
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Porcine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of swine origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in swine serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Porcine
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Porcine IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in swine serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electrondense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Porcine
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Bovine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot 1:1,000 - 1:10,000), ELISA (1:10,000 - 1:50,000=, immunoperoxidase electron microscopy and immunohistochemistry (1:500 - 1;2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation HRP
Immunogen Bovine IgG F(c) fragment

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1:1,000-10,000), ELISA (1:10,000 - 1:50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1:500-1:2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation HRP
Immunogen Bovine IgG F(ab')2 fragment.

Feline IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Cat IgG whole molecule.

Guinea pig IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/100,000-1/200,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Guinea Pig IgG whole molecule.

Bovine IgG (H+L chain) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/5,000-1/100,000, Western or dot blot), ELISA (1/10,000-1/200,000), Immunoperoxidase Electron Microscopy and Immunohistochemistry (1/500-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation HRP
Immunogen Bovine IgG whole molecule.

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-9, HRP

Applications ELISA (250 ng/ml).
Reactivities Mouse
Conjugation HRP

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications ELISA: 1/500-1/1000.
Immunohistochemistry on Frozen Sections: 1/50.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG

Peroxidase Conjugated Goat Anti-human IgA (alpha-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Human IgA
Conjugation HRP
Immunogen Human IgA, whole molecule

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, HRP

Applications ELISA: 1/2,000-1/8,000.
Reactivities Human
Conjugation HRP

Human IgG mouse monoclonal antibody, clone EFE-565, HRP, Purified

Applications Dot Blot.
ELISA.
Radioimmunoassay.
Immunohistochemistry on Frozen Sections: 1/40.
Reactivities Human
Conjugation HRP

Human IgA mouse monoclonal antibody, clone BFG-267, HRP, Purified

Applications ELISA: 1/1000.
Radioimmunoassay.
Dot Blot: 1/100.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation HRP

Mouse IgG1 goat polyclonal antibody, HRP

Applications ELISA: 1/4000-1/8000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry onParaffin Sections.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG1 paraproteins.

Mouse IgA goat polyclonal antibody, HRP

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA: 1/4000 - 1/8000.
Western Blot.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgA paraproteins

Human IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/300,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethyl-benzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and ELISA analysis.
Reactivities Human
Conjugation HRP
Immunogen Purified, non-denatured Human IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase.

Mouse IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot: 1/200 -1/5000.
Immunocytochemistry.
Immunohistochemistry: 1/250 - 1/5000.
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Mouse
Conjugation HRP
Immunogen Purified, non-denatured Mouse IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Horseradish peroxidase.

Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Rabbit
Conjugation HRP
Immunogen Purified, non-denatured Rabbit IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains) and then conjugated to Horseradish Peroxidase.

Mouse IgG3 goat polyclonal antibody, HRP

Applications

ELISA: 1/4000-1/8000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.

Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG3 paraproteins.

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP
Immunogen Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins.

Mouse IgG goat polyclonal antibody, HRP, Aff - Purified

Applications ELISA: 1/10000.
Western Blot: 1/5000-1/10000,
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP
Immunogen Purified Mouse IgG fraction

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, HRP, Aff - Purified

Applications Mouse IgG1 Peroxidase Secondary antibody reagents are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/20,000. 
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation HRP
Immunogen Native Mouse IgG1 heavy chain Protein.

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
Western blot: 1/1,000-1/10,000.
ELISA: 1/10,000-1/50,000.
Immunohistochemistry: 1/500-1/2500.
Conjugation HRP
Immunogen Chicken IgY/IgG whole molecule

Canine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Dog IgG F(c) fragment

Human IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000
Western Blot: 1/5,000-1/25,000.
Immunohistochemistry: 1/1,000-1/5,000.
Conjugation HRP
Immunogen Human IgG whole molecule

Human IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications
ELISA: 1/200,000. 
Western Blot: 1/1,000-1:5,000.
Immunohistochemistry: 1/500-1/2,500
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:32,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Human IgG whole molecule.

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. 
Recommended Dilutions: 
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/10,000.
Immunohistochemistry: 1/500-2,500.
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Human IgG F(ab')2 fragment

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, HRP

Applications Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western blotting as well as other anti IgG1 antibody based assays.
Recommended Dilutions:
ELISA: 1/300,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG1 gamma 1 heavy chain

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, HRP

Applications This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, Western-blot, Immunoprecipitation and more generally immunoassays.
Anti-Mouse IgG2a heavy chain antibody is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/90,000
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Mouse IgG2a heavy chain

Rabbit IgG (Fc specific) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/110,000.
Western Blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/5,000.
Conjugation HRP
Immunogen Rabbit IgG F(c) fragment.

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/1,500.
Conjugation HRP
Immunogen Rat IgG whole molecules.

Porcine IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/150,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Swine IgG whole molecule

Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western: 1/1,000-1/10,000 or dot blot), ELISA (1/10,000-1/50,000), immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/8,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Human IgG, IgA, and IgM whole molecules

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/10,000-1/150,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/1,000-1/10,000.
Conjugation HRP
Immunogen Chicken IgG whole molecule.

Canine IgE goat polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Western Blot: 1/1000-1/10,000.
Immunohistochemistry on Frozen Sections: 1/200-1/500.
Reactivities Canine
Conjugation HRP
Immunogen Highly purified Canine IgE

Canine IgG1 goat polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Western Blot: 1:1000 - 1:10,000.
Immunohistochemistry on frozen sections: 1:200 - 1:500.
Reactivities Canine
Conjugation HRP

Canine IgG2 rabbit polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Western Blot: 1:1000 - 1:10,000.
Immunohistochemistry on frozen sections: 1:200 - 1:500.
Reactivities Canine
Conjugation HRP

Peroxidase Conjugated Goat Anti-Mouse IgM (u-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Mouse IgM
Conjugation HRP
Immunogen Mouse IgM, whole molecule

Peroxidase Conjugated Rabbit Anti-Rat IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rat IgG
Conjugation HRP
Immunogen Rat IgG, whole molecule

Human IgA2 mouse monoclonal antibody, clone A9604D2, HRP

Applications ELISA: 1/500-1/2000.
Reactivities Human
Conjugation HRP

Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, HRP

Applications ELISA: 1/4,000-1/8,000.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG mouse monoclonal antibody, clone 2A9, HRP

Applications ELISA: 1/4,000-1/8,000.
Reactivities Rabbit
Conjugation HRP

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, HRP

Applications ELISA: 1/2,000-1/8,000.
Reactivities Human
Conjugation HRP

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, HRP

Applications ELISA: 1/4,000-1/8,000 (See also Ref.1).
Reactivities Human
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP

Rat IgG Light Chain mouse monoclonal antibody, clone KT100, HRP

Applications ELISA: 1/3000.
Western blot: 1/1000 (DAB as substrate) and 1/10000 (ECL system).
Reactivities Rat
Conjugation HRP